LOCUS       sequence-405888-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of luciferase under a synthetic promoter 
            containing two copies of the target sequence for gRNA1 (2xLuc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB396
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1553..3205
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1619..1637)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    3474..3498
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4799..5428
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5857..6930
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6996..7190
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7426..7448)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7534..7674
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7589..7608
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7689..7706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7860..8448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7940..7959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8536..9351)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8536..9351)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9259..9278
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9784..9808
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtgtttc atgtccaagc gaaaagccgc tctacggaat ggatctacgt tactgcctgc
       61 ataaggagat cggtgtagcc aaggacgaaa gcgaccctag gttctaaccg tcgactttgg
      121 cggaaaggtt tcactcagga agcagacact gattgacacg gtttagcaga acgtttgagg
      181 actaggtcaa attgagtggt ttaatatcgg catgtctggc tttaaaattc agtatagtgc
      241 gctgatcgga gatgaattaa aaacacgagt tcccaaaacc aggcgggctc gccacgtcgg
      301 ctaatcctgg tacattatgt gaacaatgtt ctgaagaaaa tttgtgaaag aaggacgggt
      361 catcgcctac tattagcaac aacggtcggc cacaccttcc attgtcgtgg ccacgctcgg
      421 attacacggc agaggtgctt gtgttccgac aggctagcat attatcctaa ggcgttaccc
      481 caatcgttta ccgtcggatt tgctatagcc cctgaacgct acatgtacga aaccatgtta
      541 tgtatgcact aggtcaacaa taggacatag ccttgtagtt aacacgtagc ccggtcgtat
      601 aagtacagta gacccttcgc cggcatccta tttattaagt tatttctaca gcaaaacgat
      661 catatgcaga tccgcagtgg gcggtagaca cacgtccacc cggctgctct gtaacaggga
      721 ctaaagaggt gatgattatc gtgtgtgccc cgttatggtc gtgttcgatc agagcgccct
      781 tgcgagcagt cgtatgcttt ctcgaattcc gagcggttaa gcgtgacagt cccagcgaac
      841 ccacaaaacg tgatcgcagt ccatgcgatc atacgcaaga aggaaggtcc ccatacaccg
      901 acgcaccagt ttacacgccg tatgcataaa cgagctgcac aaacgagagt gtttgaactg
      961 gacctctagt tcctctacaa agaacacctt gacctgttgc gaagttgcct tgcctagatg
     1021 caatgtcgga cgtatcactt ttgcctcaac gactgctgct ttcgctgtaa cccaagacag
     1081 acaacagtaa ccgccttttg aaggcaagtc ctccgcctgt gactaactgt gccaaatcgt
     1141 cttccaaacc ccttatccaa tttaactcac caaattattg cgatacagac cctaatttca
     1201 catcatatga cactaattgc ctctgccaaa attctgtcct caatgactcc ctcttctctc
     1261 accaaccagt cagtcgtgaa agtcatagta ccctgggtac caacttaccc gtcttctctc
     1321 accaaccagt ccgctggttc ctgggtgagc tcgagactcg tggtgacagc tcttcataca
     1381 tagagcggcc gcgtcgaact ccctgtcacc tttcacacag agctcatgat ctggtttata
     1441 aaggcggctt catgaccctc aattccatat agtatcactc ccatcacagc atttcgatat
     1501 cttcaaccac tttaaccttc tccagaggat catcatctca agcccttcat aaatggaaga
     1561 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     1621 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     1681 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     1741 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     1801 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     1861 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     1921 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     1981 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     2041 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     2101 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     2161 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     2221 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     2281 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     2341 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     2401 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     2461 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     2521 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     2581 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     2641 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     2701 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     2761 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     2821 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     2881 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     2941 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     3001 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     3061 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     3121 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     3181 gaagggcgga aagatcgccg tgtaatgagc ttacttaacg ttactgaaat catcaaacag
     3241 cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat
     3301 ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag
     3361 tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat
     3421 acagctcatc tgcaatgcat cgaccgtctg agacatgcgc ttgagacgaa gcttgacagg
     3481 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     3541 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     3601 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     3661 cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     3721 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     3781 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     3841 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     3901 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     3961 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     4021 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     4081 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     4141 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     4201 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     4261 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     4321 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     4381 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     4441 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     4501 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     4561 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg
     4621 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     4681 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     4741 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     4801 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     4861 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     4921 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     4981 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     5041 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     5101 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     5161 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     5221 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     5281 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     5341 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     5401 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     5461 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     5521 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     5581 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     5641 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     5701 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     5761 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
     5821 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     5881 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     5941 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     6001 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     6061 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     6121 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     6181 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     6241 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     6301 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     6361 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     6421 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     6481 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     6541 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     6601 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     6661 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     6721 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     6781 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     6841 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     6901 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     6961 ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac
     7021 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     7081 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     7141 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     7201 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     7261 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     7321 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     7381 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     7441 gctcccggtg acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     7501 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     7561 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7621 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     7681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8281 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8521 atgcattcta ggtgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     8581 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     8641 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     8701 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     8761 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     8821 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     8881 attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt
     8941 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     9001 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga tcgcagtggt
     9061 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     9121 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     9181 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt agattgtcgc
     9241 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     9301 ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca taacagaact
     9361 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     9421 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     9481 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     9541 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     9601 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     9661 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     9721 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     9781 tggtggcagg atatattgtg gtgtaaacat aacaagcttc gtctcagtca
//
