LOCUS       sequence-405889-        7517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Lowet E, Sheehan DJ, Chialva U, De Oliveira Pena R, Mount RA, Xiao 
            S, Zhou SL, Tseng HA, Gritton H, Shroff S, Kondabolu K, Cheung C, 
            Wang Y, Piatkevich KD, Boyden ES, Mertz J, Hasselmo ME, Rotstein HG,
            Han X
  TITLE     Theta and gamma rhythmic coding through two spike output modes in 
            the hippocampus during spatial navigation.
  JOURNAL   Cell Rep. 2023 Aug 29;42(8):112906. doi: 
            10.1016/j.celrep.2023.112906. Epub 2023 Aug 3.
  PUBMED    37540599
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-08-29"; volume: "42(8):112906. doi"; pages: " 
            10.1016/j.celrep.2023.112906. Epub 2023 Aug 3"
COMMENT     Sequence Label: pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1
FEATURES             Location/Qualifiers
     source          1..7517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   58..187
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    243..291
                     /note="synthetic polyadenylation signal"
     misc_feature    305..396
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     345..364
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        432..879
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      888..897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1761..2471
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2727..2783
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2784..3647
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     misc_feature    3921..4509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3974..3994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4392..4403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4542..4559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4548..4755
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4764..4893
                     /label=AAV2 ITR
     rep_origin      5002..5457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5089..5108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5299..5320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5524..5543)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5643..5665
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5703..5721)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5789..5893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5894..5962,5963..6754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6112..6131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6925..7513
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7414..7433
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct
       61 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
      121 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      181 ggttcctgcg gccgcacgcg ttctagaaat attaaggtac gggaggtact tggagcggcc
      241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
      301 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      361 tccccagtgc aagtgcaggt gccagaacat ttctctattc tagactcgag ctgcagaggg
      421 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac
      481 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat
      541 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct
      601 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc
      661 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt
      721 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg
      781 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg
      841 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cggatccgcc accatggttt
      901 ctatcgcact gcaggctggt tatgacctcc tcggagatgg tagacctgaa agtctgtggc
      961 tcggaattgg tacactgctg atgctgatcg gaaccttcta cttcctggtt agagcgtggg
     1021 gagagactga caaggatgca cgggagtact acgcagtgac aatcctggtc agcggtattg
     1081 ccagtgcagc ttatctgtca atgttctttg gtattggcct cacagaggtg ccagtaggtg
     1141 gagagatgct gaacatctac tacgcaagat acgcacagtg gctgttcact acaccactgt
     1201 tgctgctgca cctggcactc ctggccaaag ttgatagagt gaccatcggc actctcgttg
     1261 gcgtcgatgc tctgatgatt gtcacaggcc tgataggaac actgtcacat actgccattg
     1321 ccagatactc ttggtggctg ttctcaacta tctgtatgat cgtcgttctg tatgtcctgg
     1381 ccacttctct gcggtcagcc gctaaggaga gaggcccaga ggttgcctca accttcaaca
     1441 tcctcaccgc actggttctg gtcctgtgga ctgcttaccc tatcatctgg attatcggca
     1501 ccgagggtgc cggagttgtg ggcctgggaa tcgaaacttt gctgttcatg gtgctggacg
     1561 tcaccgccaa ggtgggcttc ggttttatcc tgctcaggtc aagagccatt ctgcaagata
     1621 cagaagcacc tgaaccttca gctggagctg ataccggtgc cgccgaccgc ccggtagtag
     1681 cagtgagcaa ggcggccgcc aagagcagga tcaccagcga gggcgagtac atccccctgg
     1741 accagatcga catcaacgtg atggtgtcta agggcgaaga gctgattaag gagaacatgc
     1801 acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg
     1861 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     1921 ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca
     1981 tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat
     2041 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
     2101 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg
     2161 gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg
     2221 ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc
     2281 tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc
     2341 ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct
     2401 acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc
     2461 acaagcttaa tggtgggagc ggaggtaccc agagccagcc tatcctgaac accaaggaga
     2521 tggcccctca gagcaagcct cctgaggagc tggagatgag cagcatgcct agccctgtgg
     2581 cccctctgcc tgccaggacc gagggcgtga tcgacatgag gagcatgagc agcatcgaca
     2641 gcttcatcag ctgcgccacc gacttccctg aggccaccag gttctacaag ttctgctacg
     2701 agaacgaggt gggtgtgggc tccggagcca cgaacttctc tctgttaaag caagcaggag
     2761 acgtggaaga aaaccccggt cccatgctgg gaaacggcag cgccattgtg cctatcgacc
     2821 agtgcttttg cctggcttgg accgacagcc tgggaagcga tacagagcag ctggtggcca
     2881 acatcctcca gtggttcgcc ttcggcttca gcatcctgat cctgatgttc tacgcctacc
     2941 agacttggag agccacttgc ggttgggagg aggtctacgt ctgttgcgtc gagctgacca
     3001 aggtcatcat cgagttcttc cacgagttcg acgaccccag catgctgtac ctggctaacg
     3061 gacaccgagt ccagtggctg agatacgcag agtggctgct gacttgtccc gtcatcctga
     3121 tccacctgag caacctgacc ggcctgaagg acgactacag caagcggacc atgaggctgc
     3181 tggtgtcaga cgtgggaacc atcgtgtggg gagctacaag cgccatgagc acaggctacg
     3241 tcaaggtcat cttcttcgtg ctgggttgca tctacggcgc caacaccttc ttccacgccg
     3301 ccaaggtgta tatcgagagc taccacgtgg tgccaaaggg cagacctaga accgtcgtgc
     3361 ggatcatggc ttggctgttc ttcctgtctt ggggcatgtt ccccgtgctg ttcgtcgtgg
     3421 gaccagaagg attcgacgcc atcagcgtgt acggctctac cattggccac accatcatcg
     3481 acctcatgag caagaattgt tggggcctgc tgggacacta tctgagagtg ctgatccacc
     3541 agcacatcat catctacggc gacatccgga agaagaccaa gatcaacgtg gccggcgagg
     3601 agatggaagt ggagaccatg gtggaccagg aggacgagga gacagtgctt aagggtggga
     3661 gcggaggtac ccagagccag cctatcctga acaccaagga gatggcccct cagagcaagc
     3721 ctcctgagga gctggagatg agcagcatgc ctagccctgt ggcccctctg cctgccagga
     3781 ccgagggcgt gatcgacatg aggagcatga gcagcatcga cagcttcatc agctgcgcca
     3841 ccgacttccc tgaggccacc aggttctaca agttctgcta cgagaacgag gtgtaagaat
     3901 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3961 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4021 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4081 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4141 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4201 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4261 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4321 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgcccttct
     4381 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4441 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4501 cctccccgca tcgataccgt cgactagagc tcgctgatca gcctcgactg tgccttctag
     4561 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4621 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4681 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4741 caggcatgct ggggagagat ctgaggaacc cctagtgatg gagttggcca ctccctctct
     4801 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4861 ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga gtggcccccc cccccccccc
     4921 ccccctgcag cctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4981 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5041 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5101 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5161 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5221 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5281 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5341 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5401 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     5461 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     5521 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5581 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5641 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5701 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5761 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7501 gagcctatgg aaaaacg
//
