LOCUS       sequence-405891-       10172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of geneticin resistance and 
            Nanoluciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB367
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(286..1080)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(397..416)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1007..1026
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    3820..3844
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5145..5774
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6203..7276
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7342..7536
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7772..7794)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7880..8020
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7935..7954
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8035..8052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8206..8794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8286..8305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8882..9697)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8882..9697)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9605..9624
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10130..10154
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagagcgtca tcatgcaaca tgcatgtact gtctgatgta ttaagagtat aggggtcaac
       61 aactagagtg tctgacaggt atatctagca gtttgatttg accattcttt tattaaaaca
      121 ctgtaacccg caacgaagaa ctcctccgct tcaagtcaag aatgcgcata tgtgcgtaaa
      181 atcgacaagc ccggcggcat tccaccgagc agggtattgc ctttggcact ttacgtgcgc
      241 agaggagcct gaatgttgag tggaatgatg gtggaatgat gaagctcaga agaactcgtc
      301 aagaaggcga tagaaggcta tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
      361 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
      421 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
      481 attttccacc atgatattcg gcaagcaggc gtcgccgtgg gtcacgacga gatcctcgcc
      541 gtcgggcatc cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
      601 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
      661 gcggtgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
      721 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
      781 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
      841 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
      901 gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
      961 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     1021 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     1081 tttggatgct tgggtagaat aggtaagtca gattgaatct gaaataaagg gaggaagggc
     1141 gaacttaaga aggtatgacc gggtcgtcca cttaccttgc ttgacaaacg caccaagtta
     1201 tcgtgcacca agcagcagat gataataatg tcctcgttcc tgtctgctaa taagagtcac
     1261 acttcgagcg ccgccgctac tgctacaagt ggggctgatc tgaccagttg cctaaatgaa
     1321 ccatcttgtc aaacgacaca aattttgtgc tcaccgcctg gacgactaaa ccaaaatagg
     1381 cattcattgt tgacctccac tagctccagc caagcccaaa aaatgctcct tcaatatcag
     1441 ttctcctgac ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta
     1501 ataagcgcca ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat
     1561 ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa
     1621 gaatctattg catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg
     1681 agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac
     1741 agaagtccaa ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta
     1801 ggtagagcga gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg
     1861 tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga
     1921 gctggccagc ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca
     1981 ctcgacctgc tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg
     2041 tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct
     2101 gctctcccca ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat
     2161 cttcccatcc aagaaccttt aaaatggttt tcacccttga agatttcgtc ggcgactggc
     2221 gccaaaccgc cggatataac cttgaccagg tgctggagca gggaggagtc tcttccttgt
     2281 tccagaatct cggcgtttcc gttactccta tccagcgtat tgtgttgagc ggagagaacg
     2341 gtcttaagat tgacatccac gtcattatcc catacgaggg cctctctggc gatcaaatgg
     2401 gacagatcga gaaaattttc aaggtcgttt atccagtcga cgatcatcac tttaaagtta
     2461 tcctgcacta cggtactctt gtgatcgatg gcgtcacccc caacatgatt gactacttcg
     2521 gccgacccta cgagggtatc gctgtgttcg acggtaagaa gattactgtg accggtactt
     2581 tgtggaatgg aaacaagatt atcgatgagc gcctcatcaa ccctgatggc tccttgctgt
     2641 ttcgtgttac cattaacgga gttactggtt ggcgcctttg cgaacgtatt ctcgcataag
     2701 cttcatcatt ccaccatcat tccactcaac attcaggctc ctctgcgcac gtaaagtgcc
     2761 aaaggcaata ccctgctcgg tggaatgccg ccgggcttgt cgattttacg cacatatgcg
     2821 cattcttgac ttgaagcgga ggagttcttc gttgcgggtt acagtgtttt aataaaagaa
     2881 tggtcaaatc aaactgctag atatacctgt cagacactct agttgttgac ccctatactc
     2941 ttaatacatc agacagtaca tgcatgttgc atgatgacgc tctccgtcag gagtggctat
     3001 tgttgttatc atcaaaatct atattgatac tattatactc gatatgttat agactttatt
     3061 aaaaaaaact ttgtttgttt gcagaaagtg gatttttaat caaatttaat tgtgatgatt
     3121 acaatgtcaa ttggttatgc aagatattaa ttaatatgtt taagattttt cacttttaat
     3181 aagtaaaata aaatacgtca aaaaaatttc ctaaagataa tgccaaaaag gttaaattat
     3241 agaaattaaa ctaaaatata ttaaaagttg acttaaaaat gcaatgttga ccaccgacct
     3301 aggggaaaac aaatttaccg tatctgggat agaatctctt ctgtatcatc tcagataaag
     3361 attactacat aaacacactt tacagtttta cgacgcccaa aattcaattc ccggagatta
     3421 aaaaccctaa aacaaacact ggcgattcga ttttccctcc caattcaaat catctcttaa
     3481 tcttcttctt ctcactatcc tctcaaattc gccgccgatc ttccatcaat tttgatttct
     3541 cgcaccgaaa tttcgacttt tcatctccta tcgacgaaac cctaattttt cacactccag
     3601 aaatcgaact ttgacgacga tgttaatggc taaaccccgc tgtcaggagt ctcaatggta
     3661 actttactct ttatttaacc atacattttt ttttattttt ttcactttgt tcttcatcca
     3721 ctattgttct ttgttcatct tgaacaaaag ctccctcctt ctttgttctt catccaccat
     3781 tgttcttcat caatcatttc gctgtcatga gacgaattct gacaggatat attggcgggt
     3841 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     3901 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3961 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     4021 gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4081 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     4141 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     4201 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     4261 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     4321 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     4381 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     4441 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     4501 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     4561 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     4621 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     4681 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     4741 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     4801 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     4861 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     4921 tacgtgttcg agccgcccgc gcacctctca accgtgcggc tgcatgaaat cctggccggt
     4981 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     5041 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     5101 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     5161 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     5221 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     5281 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     5341 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     5401 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     5461 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     5521 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     5581 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     5641 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     5701 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     5761 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     5821 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     5881 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     5941 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     6001 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6061 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     6121 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     6181 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     6241 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     6301 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     6361 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     6421 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     6481 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     6541 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     6601 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     6661 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     6721 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     6781 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     6841 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     6901 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6961 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     7021 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7081 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     7141 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     7201 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     7261 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     7321 aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     7381 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     7441 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     7501 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     7561 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     7621 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     7681 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     7741 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggtgacgg
     7801 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7861 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     7921 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7981 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     8041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggtg
     8881 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     8941 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     9001 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     9061 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     9121 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     9181 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     9241 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     9301 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     9361 attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat
     9421 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     9481 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     9541 acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct gattgcccga
     9601 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     9661 gccttgagca agacgtttcc cgttgaatat ggctcataac agaacttatt atttccttcc
     9721 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     9781 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     9841 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     9901 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     9961 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    10021 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    10081 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    10141 attgtggtgt aaacataacg aattcgtctc ag
//
