LOCUS       sequence-405892-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of the ubiquitin ligase gene from P. digitatum 
            (PDIG_07760)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB408
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      765..822
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(846..863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1017..1605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1097..1116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1788..1882)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1903..2562)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2477..2496
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2724..2742
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2746..2789)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2792..2813
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggaggataat ggtgattcgg cgcgtactac agcgcgtggc aggataatag gactcttgac
       61 ttgctcgaaa ttcaggtgtt acccaggctg acaaaagtct ctacgggctc aaggcgctaa
      121 tgtaatacac aagaagacgg cacaacagcg tcactgtcta gcccacggat ttgcttgatc
      181 gaatagaaga atcgatctag tctctgcgga gtgtttcttg gagtgacgcg cggtggtgaa
      241 gacacgcgct caagcaagtg atatggtgga ctgtgcaatc ccttggtgcg cgtcaaatcg
      301 acacttggcg ctaagcgggg tcgcgtgaag tgacttgagc gcaaatcacg caccttccag
      361 tcaaaattga cactagatga tttggactga aattggaaca gtatttgctc caagatcaag
      421 tgacgaaagc aacaacgcca aaagaagaat tggtaaaaga aaaaaagatg cgatgcgttg
      481 tgaggagaag acagatgagt aagtggcttg gctcccgtct ttgtcattca ccggggcatc
      541 atcacgtgtc atttgtcacc agccacaggt tcgcggcgaa atgcccttcg gtgaatctta
      601 agattgagct tctccaggtt ccccactctt catcacagac tcttttattc ccaccagttt
      661 gattctgaat tcatttcact ttaaaaatct cccatcctga aaactactca cactcaagcg
      721 aataatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc aaaaaagggc
      781 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
      841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1021 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1441 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1741 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
     1801 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     1861 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     1921 ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1981 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     2041 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     2101 ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa accctttagg
     2161 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     2221 ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt gctcatggaa
     2281 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     2341 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     2401 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2461 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2521 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     2581 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     2641 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     2701 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     2761 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagcgatgag
     2821 tggtctcg
//
