LOCUS       sequence-405895-        2787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of the elongation factor 1-alpha gene from P. digitatum 
            (PDIG_59570)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB407
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      722..779
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(803..820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(974..1562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1054..1073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1745..1839)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1860..2519)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2434..2453
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2681..2699
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2703..2746)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2749..2770
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aggtgaaaaa acggatgggg acatccaata ttgccacagt aattcgtaca ggcccaaaga
       61 aagcagaaaa aaagcacaaa gtacggtgta aacagtgcaa actgcgttgt agaagtttct
      121 cacccaacca ctctgcgttg ctgcccagtt tcaagcgttt agagccacaa agtgggctgg
      181 tacggagcag atgaaaaaaa aaaaaatttg cagagggtgg agtgtcatgg caggacacaa
      241 ccagggaaac ggaggggtgt ttcaagaaca gaagtagaca gtacatcgta aggtatggtt
      301 gccaacatgt tgaagcaagt acatctacct cagccgacca atccactttg caaaatctgc
      361 gtacacgggt aagggctaaa gccaatcagg agccctaccc acagagggct tggccccacc
      421 tgctgactcg cctcgctcgc ttgcccctcc atataaggat ttcccctcct gccctcccac
      481 agagttttct ttcttcctct tgtctgctac agtgagtttt ttctcccccc cctactgcta
      541 ttcagctctc tcttccgact acttttgctg attactattg ctcagtacct ctacgcgttc
      601 tgccgtagtc tctgagcaag tttttccaca gattttttct agtatcttac aaagaactac
      661 aaaaagttac aaccgtcatc aatgtgagac cactcatcgc gactagtagc ggccgctgca
      721 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
      781 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      961 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1741 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1801 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1861 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1921 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1981 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2041 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
     2101 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2161 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
     2221 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2281 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2341 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2401 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2461 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2521 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2581 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     2641 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     2701 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     2761 gcttctagag cgatgagtgg tctcggg
//
