LOCUS       sequence-405899-        2759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of the glucoamylase gene from A. niger, 
            maltose/starch-inducible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB405
FEATURES             Location/Qualifiers
     source          1..2759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      696..753
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(777..794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(948..1536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1028..1047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1719..1813)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1834..2493)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2408..2427
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2655..2673
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2677..2720)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2723..2744
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagtgccat tggcggaggg gtccggacgg tcaggaactt agccttatga gatgaatgat
       61 ggacgtgtct ggcctcggaa aaggatatat ggggatcata atagtactag ccatattaat
      121 gaagggcata taccacgcgt tggacctgcg ttatagcttc ccgttagtta tagtaccatc
      181 gttataccag ccaatcaagt caccacgcac gaccggggac ggcgaatccc cgggaattga
      241 aagaaattgc atcccaggcc agtgaggcca gcgattggcc acctctccaa ggcacagggc
      301 cattctgcag cgctggtgga ttcatcgcaa tttcccccgg cccggcccga caccgctata
      361 ggctggttct cccacaccat cggagattcg tcgcctaatg tctcgtccgt tcacaagctg
      421 aagagcttga agtggcgaga tgtctctgca ggaattcaag ctagatgcta agcgatattg
      481 catggcaata tgtgttgatg catgtgcttc ttccttcagc ttcccctcgt gcagatgagg
      541 tttggctata aattgaagtg gttggtcggg gttccgtgag gggctgaagt gcttcctccc
      601 ttttagacgc aactgagagc ctgagcttca tccccagcat cattacacct cagcaatgtg
      661 agaccactca tcgcgactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca
      721 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      781 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      841 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      961 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1021 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1081 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1141 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1201 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1261 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1321 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1381 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1441 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1501 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1561 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1621 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1681 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc
     1741 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1801 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcgtcgca
     1861 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1921 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1981 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     2041 cacccaggga ttggctgata cgaaaaacat attctcaata aaccctttag ggaaataggc
     2101 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     2161 gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     2221 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     2281 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     2341 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2401 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2461 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2521 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2581 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga
     2641 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     2701 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagcgatga gtggtctcg
//
