LOCUS       sequence-405923-        3071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pomoter of antifungal protein afpB gene from P. digitatum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB404
FEATURES             Location/Qualifiers
     source          1..3071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1008..1065
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1089..1106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1260..1848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1340..1359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2031..2125)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2146..2805)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2720..2739
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2967..2985
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2989..3032)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3035..3056
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagatgaat tccaccgaat gcactattat tccagagcag agtgctacaa ggaaaagaaa
       61 cggggtttct aattcatggt ttctttttct tacactgccg tccccagtca taattctgtc
      121 tggatcctgc caatcttttt ttaaagatca ccattgttga gacatcacgt tgtcgtttat
      181 aggattcact atttgttttg agccggccca cttgcttgtg aattacgtta catacagagc
      241 attgcggact gaaattgatg cacccgccca cgaggtgctt attcccaagg tttttgcgag
      301 cctctggcgg atatgcagct aattctcgcc acttgcttgt tttgtacttg tatatccaga
      361 tcttacttac tttcactttg ccctagttcg tcggactttc accccaagct tgcagcaggc
      421 cacaaggctt tgcatgcagg gactgcggtt cgaccgatgg gggcagatca gcagtatctt
      481 tagcattgag ccaatgaaag cgttgtcgat cctatacccc gcatgtatag gatgctcctc
      541 tatctgggga tctgaaggtc actcctcttc caacatcacc gaggatgtaa ggtcacccag
      601 aacttcaata ttttactatc ggattggttg cttcaatcct tctgcatggg gtatcacacc
      661 ccaacgtgat acaattacca ataactcaac tgtttcacct aaaagatcat accaactaac
      721 gagttgagtt catttggatt tatgaggcat accttttatt ggacagcttg gatatctttc
      781 gaccacagtt ccaatgatta ctgcactatt ttcgaggaca gtacttgtca tatattgtag
      841 gaagcatccg gcaatttcta tataagatac cttcgcggcg ctccaattta caaagaaacg
      901 atcgcattaa cattacttct ctctcaacta ccataccaca atcttcgata agaccacact
      961 ttcacgaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag
     1021 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     1081 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1261 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1981 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     2041 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     2101 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     2161 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     2221 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2281 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2341 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt
     2401 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2461 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg
     2521 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2581 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2641 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2701 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2761 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2821 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2881 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     2941 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     3001 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat
     3061 gagtggtctc g
//
