LOCUS       sequence-405924-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of the highly-expressed pyruvate kinase gene from A. 
            niger..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB389
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1084..1141
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1165..1182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1336..1924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1416..1435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2107..2201)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2222..2881)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2796..2815
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3043..3061
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3065..3108)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3111..3132
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagtaaatg gaagagaaaa cctccgagta cttacttagg gccctgtcta ctggccagag
       61 tctcgtcctc tattcactat gataaattac ccactggaca aaaaaataaa ataaaataaa
      121 aataaaaagg gagacagctt ctccatcaac tggcaactgg gtccgtccga gcagagcaaa
      181 attcagcctt atgggttccg atggagtcag ggaaatagtt cttgcgaagg gcattgggct
      241 tttttgcgag gagaaaattc agcaccgaca aagcatccga aatccgacct cgctaggaga
      301 gaatggatcc gcgacgatgt ggggtcaact ggacagagtg agagggtatc atgtggtcct
      361 gccagatact tcgcagaatg ttgtgtgggt gtctgattgt ggcttgggcg tgaattgctt
      421 ttggtcttcc caaccaatta ttattgcatg cggcgtatga atgcctgaga tgcgcggagg
      481 gaaggtgcct gaggatgtag tggacaaatg ctgctgatcg ctgggcggaa acccttggct
      541 gaccagtgaa aagagcggac ggaggcagca ggtgtatcta cgatcaaaga atagtagcaa
      601 agcagtgaaa ggtggatcac ccagcaaata attgagtttt gatacccagc gatagtgccg
      661 ggggggagaa aaagtcatta ataatgggaa ttatgtaggc gatgggaagt gtgattgtaa
      721 ctactccgta gctggaggca caactaacaa gccagctctc aacccgcggg gaaccgaccg
      781 acagataaaa aaaagcgtcc caaagcagga atcccaccaa aaagggccga tccagccaat
      841 caccgccgcc aacatttttc cttcccgggc acccctcctc tagtccacca tctctctctt
      901 ctctcgctca ccggccccgt cttttccttc cctattatct ctccctcttc tcctcccttc
      961 tctccctcca ttctttctcc catcttcatc actcccttct cttctgtctt cccccccggt
     1021 tcagtagaga tcaatcagct agaatgtgag accactcatc gcgactagta gcggccgctg
     1081 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1141 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1321 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     2101 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2161 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2221 attacgcccc gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga
     2281 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2341 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2401 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat
     2461 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2521 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg
     2581 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2641 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2701 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2761 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2821 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2881 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2941 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     3001 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3061 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     3121 ccgcttctag agcgatgagt ggtctcg
//
