LOCUS       sequence-405965-        2773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter of the endo-1,4-beta-xylanase A gene from A. nidulans, 
            xylose-inducible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB291
FEATURES             Location/Qualifiers
     source          1..2773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     191..210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     444..461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(485..542)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    1267..1288
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1291..1334
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1338..1356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1518..2177
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1584..1603)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2198..2292
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(2475..2773,1..290)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctgtggaaa aacgccagca
      301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa
      481 gtaatctttt cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg
      541 gactgcagcg gccgctacta gtcgcgatga gtggtctcac attcattgtg ctgatcctgt
      601 cagttgggtt gttggaatct gggagccgga cagaaaggaa tgctacctcc tttataggta
      661 gtgtccggtt aatagcctct ggatcagaag tacaaagtct ccccgtgaga gggcaatcag
      721 cctgtttccg gctcttttgg ttataaactc ctgaatagcc attcgttttc ccagatagcg
      781 tagcagcaac tgcaagctgt gcatgtgcta gagcccatgc gggcaaagaa aaaccccaga
      841 ctaaacccag tggaccccga tccgctccac aaaccgaatt gctgaatagc ctgaatgaag
      901 aatctctgca gaattctatt gctccttgtt ccagtgggaa acggcgccgc ttggcacgct
      961 taagtatcat ggttcagctc cgtttcaaat agaggcagct ccggcgactg ttctagaccc
     1021 gtgggccaag acgagtccat ctgtctgtaa tgctatcgga agctattgga tggcccctga
     1081 gcatcaggta gatgaggttt ccggctctca atcagagttt agggcaatcg gccggtacac
     1141 gttcaatgag aactccagct taggcataga tgtcctacca tagccaccgc tagaccactt
     1201 actgggtact acattttcgg ggaataatga tcaacgcact tccaggactc ccgagaccac
     1261 tcatcgctct agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt
     1321 tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg tcatgataat
     1381 aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga ggttccaact
     1441 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1501 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1561 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1621 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1681 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     1741 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1801 tccatgagca aactgaaacg ttttcatcac tctggagtga ataccacgac gatttccggc
     1861 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1921 ctaaagggtt tattgagaat atgtttttcg tatcagccaa tccctgggtg agtttcacca
     1981 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     2041 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2101 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgacgagt
     2161 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
     2221 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2281 attggtgaga atccaagcct cgagctgtca gaccaagttt actcatatat actttagatt
     2341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2761 tagttaccgg ata
//
