LOCUS       sequence-405982-       10620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Membrane protein fusion with extracellular SNAPtag and intracellular
            FKBP(f36v) dimerization domain and GFP reporter under 
            Tetracycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, 
            Cumberworth A, Stehr F, Jungmann R, Schwille P, Ganzinger KA
  TITLE     Dual-color DNA-PAINT single-particle tracking enables extended 
            studies of membrane protein interactions.
  JOURNAL   Nat Commun. 2023 Jul 19;14(1):4345. doi: 10.1038/s41467-023-40065-8.
  PUBMED    37468504
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-07-19"; volume: "14(1):4345. doi"; pages: " 
            10.1038/s41467-023-40065-8"
COMMENT     Sequence Label: pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)
FEATURES             Location/Qualifiers
     source          1..10620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(159..176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     386..405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             585..1184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(585..604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1081..1101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1312..1545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1617..1738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1654..1673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1708..1727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1778..1913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1934..1952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1962..1979)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1962..1978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1971..1993)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2120..2575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2207..2226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2417..2438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2601..2705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2706..2774,2775..3566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2924..2943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3737..4325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4226..4245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4479..4496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4613..4634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4649..4654,4655..4672,4673..4679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4687..4703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4692..4714
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4711..4727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4711..4727
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4746..4766
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4748..4766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4794..5020
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5021..5201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5248..5373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5866..6099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6284..6328
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6617..6711
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     6628..6647
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    6665..6683
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6693..6711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6778..6804
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7519..7521,7522..7524,7525..8235)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7519..7521,7522..7524,7525..8235)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7564..7585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7825..7844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8172..8193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8616..8733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8782..9292
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9211..9229
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          9301..9873
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9881..9900
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        9928..9946
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9957..10577
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(10030..10049)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    join(10612..10620,1..565)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
       61 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
      121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
      181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
      241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
      301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
      361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
      421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
      481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      541 tttcctttga aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc
      601 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
      661 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
      721 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
      781 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
      841 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
      901 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
      961 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     1021 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     1081 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     1141 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     1201 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga
     1261 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     1321 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     1381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     1561 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     1621 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1681 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1741 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     1801 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     1861 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1921 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     1981 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2041 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2101 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2161 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2221 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2281 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2341 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2401 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2461 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2521 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     2581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3721 agatcaaagg atcttcttga gatccttttt ttctgtgcgt aatctgctgc ttgcaaacaa
     3781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4381 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4441 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4501 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4561 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4621 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4681 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4741 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     4801 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4861 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4921 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     4981 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     5041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5161 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     5221 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     5281 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     5341 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     5401 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     5461 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     5521 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     5581 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     5641 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     5701 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     5761 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     5821 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5881 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5941 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     6001 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     6061 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     6121 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     6181 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     6241 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     6301 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     6361 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     6421 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     6481 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     6541 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta
     6601 gcctcgagcg gccgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt
     6661 gaaatcccta tcagtgatag agacttataa gttccctatc agtgatagag acaccggtcg
     6721 cgtgccacca tgaagaccat catcgccctg agctacatct tctgcctggt gttcgcctac
     6781 ccatacgatg ttccagatta cgctatggac aaagattgcg aaatgaagcg caccaccctg
     6841 gatagccctc tgggcaaact ggaactgtct gggtgcgaac agggcctgca ccgtatcatc
     6901 ttcctgggca aaggaacatc tgcagctgac gccgttgaag tgcctgctcc agccgcagtg
     6961 ctgggcggac cagagccact gatgctggcc accgcctggc tcaacgccta ctttcaccag
     7021 cctgaggcca tcgaggagtt ccctgtgcca gccctgcacc acccagtgtt ccagcaggag
     7081 agctttaccc gccaggtgct gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc
     7141 agctacagcc acctggctgc cctggcaggc aatcccgcag ccacagctgc agtgaaaaca
     7201 gctctgagcg gaaatcccgt gcccattctg atcccctgcc accgggttgt acagggagac
     7261 ctggatgttg gaggttacga gggcggactc gccgtgaaag agtggctgct ggcccacgag
     7321 ggccacagac tgggcaagcc tgggctgggt ggtagtggtg gatcccggct tttatcttca
     7381 cctttctcta tagagcttga ggaccctcag cctcccccag accacattcc ttggattaca
     7441 gctgtacttc caacagttat tatatgtgtg atggttttct gtctaattct atggaaatgg
     7501 aagaaaaaga agcggcctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     7561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     7621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     7681 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     7981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     8041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     8101 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     8161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     8221 gacgagctgt acaagactag tggatccggt gtgcaggtcg agacaatttc tcctggcgac
     8281 ggccggacat tccctaaacg aggacagaca tgcgtggtgc actacaccgg catgctggaa
     8341 gatggcaaga aggtggacag cagccgggat agaaacaaac ccttcaagtt catgctgggc
     8401 aagcaagaag tgatcagagg ttgggaagag ggcgtcgctc agatgtctgt tggacagaga
     8461 gccaagctga caatcagccc cgattacgcc tatggcgcca caggacaccc tggcatcatt
     8521 cctccacatg ctacactggt gttcgacgtg gaactgctga agctggaata aagcgaattc
     8581 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     8641 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8701 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     8761 ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt
     8821 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     8881 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     8941 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     9001 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     9061 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     9121 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     9181 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     9241 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatct
     9301 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     9361 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     9421 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     9481 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     9541 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     9601 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     9661 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     9721 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa
     9781 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     9841 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     9901 ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga attgatatgt
     9961 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
    10021 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
    10081 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
    10141 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
    10201 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
    10261 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
    10321 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
    10381 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
    10441 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
    10501 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
    10561 aatgtgaaag tgggtccgcg tacagcggct cccgggagtt ctagggatct gcccctctcc
//
