LOCUS       sequence-405983-        3255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence of pyr4 gene from T. reesei..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB272
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(477..571)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(592..1251)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1166..1185
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1413..1431
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1435..1478)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1481..1502
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2709..2766
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2790..2807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2961..3255,1..294))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3041..3060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
       61 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
      481 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      541 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      601 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      661 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      721 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      781 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
      841 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      901 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg
      961 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1021 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1081 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     1141 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     1201 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     1261 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     1321 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     1381 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
     1441 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
     1501 agcgatgagt ggtctcgaat gatggcacca cacccgacgc tcaaggccac cttcgcggcc
     1561 aggagcgaga ctgcgacgca cccgctgacg gcttacctgt tcaagctcat ggacctcaag
     1621 gcatccaacc tgtgcctgag cgccgacgtg ccgacagcgc gcgagctgct gtacctggcc
     1681 gacaagattg gtccgtcgat tgtcgtgctc aagacgcact acgacatggt ttcgggctgg
     1741 gacttccacc cggagactgg cacgggagcc cagctggcgt cgctggctcg caagcacggc
     1801 ttcctcatct tcgaggaccg caagtttggt gacattggcc acaccgtcga gctgcagtac
     1861 acgggtgggt cggctcgcat catcgactgg gctcacattg tcaacgtcaa catggtgccc
     1921 ggtaaggcgt cggtggcctc gctggcccag ggtgccaagc gctggctcga gcgctacccc
     1981 tgcgaggtca agacgtccgt caccgtcggc acgcccacca tggactcgtt tgacgacgac
     2041 gccgactcca gggacgccga gccagccggt gccgtcaacg gtatgggttc cattggtgtc
     2101 ctggacaagc ccatctactc gaaccggtcc ggcgacggcc gcaagggaag catcgtttcc
     2161 atcaccaccg tcacccagca gtacgagtcc gtttcctcgc cacggttaac aaaggccatc
     2221 gccgagggtg acgagtcgct cttcccggga atcgaggagg ctccgctgag ccgcggcctc
     2281 ctgatcctcg cccaaatgtc cagccagggc aacttcatga acaaggagta cacgcaggcc
     2341 tgcgtcgagg ccgcccggga gcacaaggac tttgtcatgg gcttcatctc gcaggagact
     2401 ctcaacaccg agcccgacga tgcctttatc cacatgacgc ccggctgcca gctgcccccc
     2461 gaagacgagg accagcagac caacggatcg gtcggtggag atggccaggg ccagcagtac
     2521 aacacgccgc acaagctgat tggcatcgcc ggcagcgaca ttgccattgt gggccggggc
     2581 atcctcaagg cctcagaccc cgtagaggag gcagagcggt accgatcagc agcgtggaaa
     2641 gcctacaccg agaggctgct gcgataggct ttgagaccac tcatcgcgac tagtagcggc
     2701 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     2761 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2941 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     3001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3241 cgctgcgcct tatcc
//
