LOCUS       sequence-405985-       11141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Membrane protein fusion with extracellular GFP and intracellular 
            FKBP(f36v) dimerization domain and HALOtag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, 
            Cumberworth A, Stehr F, Jungmann R, Schwille P, Ganzinger KA
  TITLE     Dual-color DNA-PAINT single-particle tracking enables extended 
            studies of membrane protein interactions.
  JOURNAL   Nat Commun. 2023 Jul 19;14(1):4345. doi: 10.1038/s41467-023-40065-8.
  PUBMED    37468504
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-07-19"; volume: "14(1):4345. doi"; pages: " 
            10.1038/s41467-023-40065-8"
COMMENT     Sequence Label: pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)
FEATURES             Location/Qualifiers
     source          1..11141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(192..212)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        197..526
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      377..512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1660..1725
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2300..2434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2337..2356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2391..2410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2602..3235
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3282..3407
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3904..4137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4322..4366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4651..4768
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4921..5328
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             5416..5442
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5443..5445,5446..5448,5449..6159)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5443..5445,5446..5448,5449..6159)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5488..5509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5749..5768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6096..6117
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6328..7212
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    7606..8194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7659..7679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8077..8088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8197..8213)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8198..8214)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8304..8537
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8562..8580)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8563..8580)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8870..8889)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9103..9122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9222..9244
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9282..9300)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9368..9472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9473..9541,9542..10333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9691..9710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10504..11092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10993..11012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
       61 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      121 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      181 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      301 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
      481 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
      541 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
      601 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
      661 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
      721 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
      781 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
      841 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
      901 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
      961 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     1021 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     1081 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     1141 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     1201 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     1261 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     1321 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     1381 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     1441 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     1501 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     1561 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     1621 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     1681 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     1741 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     1801 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     1861 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     1921 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     1981 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     2041 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     2101 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     2161 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     2221 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2281 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2401 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     2461 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     2521 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     2581 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     2641 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     2701 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     2761 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     2821 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     2881 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     2941 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     3001 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     3061 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3121 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3181 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     3241 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     3301 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     3361 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     3421 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     3481 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     3541 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     3601 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     3661 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     3721 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     3781 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     3841 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     3901 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     3961 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     4021 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     4081 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     4141 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     4201 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     4261 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     4321 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     4381 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     4441 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     4501 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     4561 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     4621 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     4681 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     4741 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     4801 ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt
     4861 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
     4921 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     4981 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     5041 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     5101 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     5161 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     5221 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     5281 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
     5341 ctctcgagaa ttctcacgcg tgccaccatg aagaccatca tcgccctgag ctacatcttc
     5401 tgcctggtgt tcgcctaccc atacgatgtt ccagattacg ctatggtgag caagggcgag
     5461 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5521 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5581 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5641 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5701 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5761 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5821 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5881 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5941 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6001 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6061 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6121 gccgccggga tcactctcgg catggacgag ctgtacaagg gtagtggtgg atcccggctt
     6181 ttatcttcac ctttctctat agagcttgag gaccctcagc ctcccccaga ccacattcct
     6241 tggattacag ctgtacttcc aacagttatt atatgtgtga tggttttctg tctaattcta
     6301 tggaaatgga agaaaaagaa gcggcctgaa atcggtactg gctttccatt cgacccccat
     6361 tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc
     6421 cctgtgctgt tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg
     6481 catgttgcac cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac
     6541 aaaccagacc tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa
     6601 gccctgggtc tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc
     6661 cactgggcca agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc
     6721 cctatcccga cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc
     6781 accaccgacg tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg
     6841 ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc
     6901 ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt
     6961 gagccagcga acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct
     7021 gtcccgaagc tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct
     7081 cgcctggcca aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg
     7141 ctgcaagaag acaacccgga cctgatcggc agcgagatcg cgcgctggct gtctactctg
     7201 gagatttccg gtagtggatc cggtgtgcag gtcgagacaa tttctcctgg cgacggccgg
     7261 acattcccta aacgaggaca gacatgcgtg gtgcactaca ccggcatgct ggaagatggc
     7321 aagaaggtgg acagcagccg ggatagaaac aaacccttca agttcatgct gggcaagcaa
     7381 gaagtgatca gaggttggga agagggcgtc gctcagatgt ctgttggaca gagagccaag
     7441 ctgacaatca gccccgatta cgcctatggc gccacaggac accctggcat cattcctcca
     7501 catgctacac tggtgttcga cgtggaactg ctgaagctgg aataaagcgg ccgcgactct
     7561 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     7621 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7681 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7741 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7801 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7861 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7921 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7981 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8041 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8101 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8161 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     8221 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8341 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8521 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     8581 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     8641 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     8701 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     8761 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     8821 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     8881 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     8941 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     9001 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     9061 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     9121 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9181 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     9241 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     9301 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9361 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9421 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9481 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9541 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9601 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9661 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9721 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9781 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9841 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9901 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9961 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10021 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10081 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10141 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10201 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10261 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10321 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10381 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10441 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10501 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10561 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10621 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10681 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10741 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10801 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10861 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10921 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10981 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11041 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11101 caacgcggcc tttttacggt tcctggcctt ttgctggcct t
//
