LOCUS       sequence-405987-       10805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Membrane protein fusion with extracellular SNAPtag and intracellular
            FKBP(f36v) dimerization domain and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, 
            Cumberworth A, Stehr F, Jungmann R, Schwille P, Ganzinger KA
  TITLE     Dual-color DNA-PAINT single-particle tracking enables extended 
            studies of membrane protein interactions.
  JOURNAL   Nat Commun. 2023 Jul 19;14(1):4345. doi: 10.1038/s41467-023-40065-8.
  PUBMED    37468504
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-07-19"; volume: "14(1):4345. doi"; pages: " 
            10.1038/s41467-023-40065-8"
COMMENT     Sequence Label: pHR-SNAP-CD86tm-eGFP-FKBP(f36v)
FEATURES             Location/Qualifiers
     source          1..10805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    234..359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    856..1089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1274..1318
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1603..1720
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1873..2280
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2368..2394
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3109..3111,3112..3114,3115..3825)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3109..3111,3112..3114,3115..3825)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3154..3175)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3415..3434)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3762..3783
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4222..4810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4275..4295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4693..4704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4813..4829)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4814..4830)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4920..5153
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5178..5196)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5179..5196)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5486..5505)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5719..5738)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5838..5860
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5898..5916)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5984..6088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6089..6157,6158..6949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6307..6326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7120..7708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7609..7628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7862..7879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7949..7969)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7954..8283
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8134..8269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9417..9482
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9612..9632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10057..10191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10094..10113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10148..10167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10359..10805,1..187)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
       61 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      181 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      241 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      301 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      361 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      421 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
      481 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
      541 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
      601 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
      661 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
      721 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
      781 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
      841 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
      901 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
      961 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1021 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1081 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1141 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1201 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1261 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1321 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1381 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1441 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     1501 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     1561 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
     1621 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1681 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1741 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
     1801 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     1861 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
     1921 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
     1981 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
     2041 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
     2101 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
     2161 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
     2221 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
     2281 ggggatctgg agctctcgag aattctcacg cgtgccacca tgaagaccat catcgccctg
     2341 agctacatct tctgcctggt gttcgcctac ccatacgatg ttccagatta cgctatggac
     2401 aaagattgcg aaatgaagcg caccaccctg gatagccctc tgggcaaact ggaactgtct
     2461 gggtgcgaac agggcctgca ccgtatcatc ttcctgggca aaggaacatc tgcagctgac
     2521 gccgttgaag tgcctgctcc agccgcagtg ctgggcggac cagagccact gatgctggcc
     2581 accgcctggc tcaacgccta ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca
     2641 gccctgcacc acccagtgtt ccagcaggag agctttaccc gccaggtgct gtggaaactg
     2701 ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc acctggctgc cctggcaggc
     2761 aatcccgcag ccacagctgc agtgaaaaca gctctgagcg gaaatcccgt gcccattctg
     2821 atcccctgcc accgggttgt acagggagac ctggatgttg gaggttacga gggcggactc
     2881 gccgtgaaag agtggctgct ggcccacgag ggccacagac tgggcaagcc tgggctgggt
     2941 ggtagtggtg gatcccggct tttatcttca cctttctcta tagagcttga ggaccctcag
     3001 cctcccccag accacattcc ttggattaca gctgtacttc caacagttat tatatgtgtg
     3061 atggttttct gtctaattct atggaaatgg aagaaaaaga agcggcctat ggtgagcaag
     3121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3301 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3781 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagactag tggatccggt
     3841 gtgcaggtcg agacaatttc tcctggcgac ggccggacat tccctaaacg aggacagaca
     3901 tgcgtggtgc actacaccgg catgctggaa gatggcaaga aggtggacag cagccgggat
     3961 agaaacaaac ccttcaagtt catgctgggc aagcaagaag tgatcagagg ttgggaagag
     4021 ggcgtcgctc agatgtctgt tggacagaga gccaagctga caatcagccc cgattacgcc
     4081 tatggcgcca caggacaccc tggcatcatt cctccacatg ctacactggt gttcgacgtg
     4141 gaactgctga agctggaata aagcggccgc gactctagag tcgacctgca ggcatgcaag
     4201 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     4261 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4321 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4381 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4441 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4501 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4561 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4621 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4681 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4741 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4801 gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag
     4861 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4921 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5161 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     5221 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     5281 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     5341 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     5401 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     5461 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     5521 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     5581 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     5641 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     5701 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5761 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5821 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5881 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5941 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6001 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6061 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6121 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6181 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6241 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6301 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6361 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6421 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6481 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6541 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6601 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6661 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6721 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6781 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6841 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6901 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6961 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7021 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7261 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7801 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7861 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7921 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggta tggaaagtcc
     7981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8041 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8101 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8161 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8281 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     8341 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     8401 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     8461 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     8521 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     8581 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
     8641 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
     8701 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
     8761 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
     8821 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
     8881 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
     8941 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
     9001 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
     9061 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
     9121 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
     9181 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
     9241 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
     9301 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
     9361 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     9421 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     9481 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     9541 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     9601 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
     9661 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     9721 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
     9781 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
     9841 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
     9901 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
     9961 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    10021 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    10081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10201 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10261 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10321 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
    10381 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
    10441 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
    10501 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
    10561 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
    10621 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
    10681 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
    10741 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
    10801 ttgcc
//
