LOCUS       sequence-405989-        8942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of terbinafine resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB431
FEATURES             Location/Qualifiers
     source          1..8942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2586..2610
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3911..4540
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4969..6042
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6108..6302
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6538..6560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6646..6786
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6701..6720
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6801..6818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6972..7560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7052..7071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7648..8463)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7648..8463)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8371..8390
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    8896..8920
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtggcgc atgcggacag acggacggac gcagagagaa gggctgagta ataagcgcca
       61 ctgcgccaga cagctctggc ggctctgagg tgcagtggat gattattaat ccgggaccgg
      121 ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa gaatctattg
      181 catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg agaatgtgaa
      241 gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac agaagtccaa
      301 ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta ggtagagcga
      361 gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg tccaaatatc
      421 gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga gctggccagc
      481 ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca ctcgacctgc
      541 tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg tgtgtcggcg
      601 gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct gctctcccca
      661 ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat cttcccatcc
      721 aagaaccttt aaaatgacct tgctcaatgg ccacgcctcc ctctccccgg ccgctcaacg
      781 ccgcatcgaa caccatgaag ctgatattgt aattgtgggt gccggagtcc tcggctgtgc
      841 acttgcagtt gcactgggaa atcaagggcg cagtgtaatc ctgctggaga agtcgttgga
      901 agagcccaac cgtattgttg gtgaactact ccaaccggga ggtgttcagg cgctcgagca
      961 actagggcta cgggattgtc tggaagatat cgatggcatc aatgtgaagg gatactgggt
     1021 tacttacttt ggggagccgg ttctcctcga ataccccaag tccagcccaa cctcgcctac
     1081 accgttggga cgagccttcc accatggccg attcgtgatg aaactacggg cagccgctct
     1141 gtcgtgcccc aatgtcacgg tggtcgaaac caaggttacc ggcctcatca catcctcata
     1201 tactcaggaa gtccttggag tcgagtgtat aaccaaggat gtcaaggact gctacttcgg
     1261 tcaactcacc gtggccgccg acggttataa ctccgttttc cgcaaggaac accaccagta
     1321 tacacccaaa cggcgctcaa agttccatgg cttggagctc atcgatgcta aactgcccgc
     1381 tcccaacact ggtcatgtgc ttctcagcga taatccaccc gtacttatgt accagatcgg
     1441 cacccatgaa acccgaatcc ttatcgatat ccctgataac ctgcctgcgg cctcggtcaa
     1501 gaacggtggt gtcaagggtt acatgcgaaa caacatcctg cccaagctcc cagagggtgt
     1561 ccagcagtcg ttctccgatg ccctagagaa gggccagcta cggtcaatgc cgaactcgtt
     1621 cctgcctgcc tcggccaaca agacacccgg cttgatgatt ctgggcgatg cgcttaacat
     1681 gcggcaccct ctcacaggtg gaggcatgac tgtggcgctc aacgatgttt gtgtcatccg
     1741 cgaattgctc agcccagagc gcgtgcccaa cctctccaat acaggccttg ttctcgagca
     1801 gctcgcagag ttccactgga aacgaaagaa ctcatcttcg gtcatcaata tcctcgcaca
     1861 ggctctctat gcgctcttcg ctgcagacag tacgcacgct cactcttcag gttaatttgc
     1921 aataactaac tgttcattca acagactatt acctcaaagc tcttcagcgt ggctgtttcc
     1981 gctacttcca gattggacca gttggcggcc ctgtcgggtt gttagccggt ctaatcaaga
     2041 aacccctcgt ccttgtcagc cacttcttct ccgttgcctt cctcgccatc tgggtccaca
     2101 tatgcgatac ccctctgtcc aagctattcc tagccccata ctacgccgtc atgatattgt
     2161 acacagcctc tgtcgtgatt ttgccataca tctggaccga gatctggtac tgagcttcta
     2221 ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa ataagcgctg
     2281 aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac cgaagagata
     2341 tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc agtctagaca
     2401 cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg atctaaggat
     2461 ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc atgctgccac
     2521 aggtgactct ggatggcccc ataccactca acccatggta cgagcggtac gcttgagacg
     2581 aagcttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     2641 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     2701 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     2761 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa
     2821 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     2881 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     2941 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3001 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3061 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3121 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3181 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3241 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3301 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3361 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3421 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3481 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     3541 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     3601 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     3661 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg
     3721 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     3781 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     3841 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     3901 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     3961 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4021 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4081 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4141 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4201 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4261 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4321 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4381 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4441 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4501 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     4561 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     4621 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     4681 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     4741 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     4801 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     4861 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     4921 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     4981 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     5041 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     5101 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     5161 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     5221 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     5281 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     5341 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     5401 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     5461 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     5521 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     5581 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     5641 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     5701 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     5761 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     5821 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     5881 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     5941 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     6001 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     6061 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg
     6121 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     6181 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     6241 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     6301 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     6361 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     6421 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     6481 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6541 ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag
     6601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     6661 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     6721 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     6781 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7381 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7621 gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg
     7681 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     7741 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     7801 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     7861 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     7921 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     7981 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt
     8041 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     8101 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
     8161 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     8221 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     8281 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     8341 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     8401 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
     8461 cataacagaa cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     8521 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     8581 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
     8641 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     8701 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     8761 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     8821 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     8881 ctgttggctg gctggtggca ggatatattg tggtgtaaac ataacaagct tcgtctcagt
     8941 ca
//
