LOCUS       sequence-405996-       12084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express UbC-driven LARGE1 and EF-1a driven BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12084)
  TITLE     Using Saturation Mutagenesis-Reinforced Functional Assays (SMuRF) to
            improve the variant interpretation for alpha-dystroglycan 
            glycosylation enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lenti-UbC-LARGE1-EF1a-BSD
FEATURES             Location/Qualifiers
     source          1..12084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             119..299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(325..342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    331..555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      601..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(688..707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     898..919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1038..1058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1203..1337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1399..1446
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1465..1839
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1969..2090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2006..2025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2060..2079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2139..2155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2139..2155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2152..2174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2187..2193,2194..2211,2212..2217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2232..2253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2370..2387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2541..3129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2621..2640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3300..4091,4092..4160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3923..3942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4161..4265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4340..4359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4531..4910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4912..5110
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5065..5085
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5128..5308
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5355..5480
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5973..6206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6391..6435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6733..6850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6900..7299
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     promoter        9609..10787
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9840..10778
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10735..10755
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10794..10803
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10800..11198
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11223..11811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11276..11296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11694..11705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
       61 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      361 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      421 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      481 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      541 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1021 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1201 agtcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     1381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1441 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     1501 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     1561 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     1621 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     1681 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     1741 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     1801 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     1861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     1921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     1981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     2101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     4321 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     4381 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     4441 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     4501 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     4561 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4621 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4681 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4741 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4801 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4861 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4921 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4981 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     5041 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     5101 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5281 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5341 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5401 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5461 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5521 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5581 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5641 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5701 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5761 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     5821 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5881 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5941 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     6001 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     6061 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     6121 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     6181 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6241 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6301 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6361 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6421 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6481 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6541 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6601 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6661 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     6721 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6781 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6841 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagcg
     6901 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     6961 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     7021 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     7081 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     7141 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     7201 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     7261 gcggttcttg tttgtggatc gctgtgatcg tcacttggta ccggttctag aatgctggga
     7321 atctgcaggg ggagacggaa attcttggct gcctcgttga gtcttctctg catcccagcc
     7381 atcacctgga tttacctgtt ttctgggagc ttcgaagatg gaaagcccgt gtctctgtca
     7441 ccgctggagt cccaggcaca cagccccagg tacacggcct ccagccagcg ggagcgcgag
     7501 agcctggagg tgcgcatgcg cgaggtggag gaggagaacc gcgccctccg caggcagctc
     7561 agcctggccc agggccgagc cccatcccat cgccgaggca accactccaa gacctactcc
     7621 atggaggagg gcactggaga cagcgagaac cttcgggctg gcatcgtggc aggcaacagc
     7681 tccgagtgtg ggcagcagcc ggtcgtggag aaatgcgaga caatccacgt tgctattgtc
     7741 tgcgccggat acaatgccag ccgggatgtc gtcaccctgg tcaaatccgt cctgttccat
     7801 agacggaacc ctctgcactt ccaccttatt gctgactcca ttgcggagca gatcctggcc
     7861 acgctcttcc agacctggat ggtgcccgct gtgcgtgtgg acttctacaa tgcagacgag
     7921 ctcaagtctg aagtttcctg gatccccaat aaacattact ctgggattta tggtctgatg
     7981 aagcttgtcc tgaccaagac tcttcctgcc aacctggaga gagtcatcgt ccttgacacg
     8041 gatatcacct ttgccactga cattgcagag ctgtgggctg tgttccacaa gttcaaaggt
     8101 cagcaagtcc tgggcttggt ggagaaccag agtgactggt accttggaaa cctgtggaaa
     8161 aatcaccgcc catggccagc ccttggaaga ggctacaaca caggggtgat cctgttactt
     8221 ctggataagc tgcggaagat gaaatgggag cagatgtgga ggctgaccgc agagagggag
     8281 ctcatgggca tgctctctac atccttagct gaccaggata ttttcaatgc cgtcatcaaa
     8341 caaaacccct tccttgtgta ccagctcccc tgcttctgga atgtgcagct gtcagaccac
     8401 acccgctccg agcagtgcta cagagacgtg tctgatctaa aggtcattca ctggaactcc
     8461 cccaagaagc tccgggtgaa gaacaagcat gtggagtttt ttcgcaacct ctacctgacc
     8521 ttcctggagt atgacggcaa tcttctgagg cgggaactgt ttggctgccc cagtgaggct
     8581 gatgtcaaca gtgaaaacct ccagaagcag ctgtctgagc tggacgagga cgacctgtgc
     8641 tatgagttcc ggcgagagcg cttcactgtc caccgcaccc acctgtactt cctgcactac
     8701 gagtatgagc ctgcagcaga cagcacggac gtcaccctgg tcgctcagct gtccatggac
     8761 aggctccaga tgctggaggc catctgcaag cactgggagg ggcccatcag cctggccctc
     8821 tacctgtcag acgccgaggc ccagcagttc ctccgctacg cacagggctc tgaggtgctt
     8881 atgagccgcc acaacgtggg ctaccacatc gtgtacaagg agggccagtt ctaccccgtg
     8941 aacctgctgc gcaacgtggc catgaagcac atcagcactc cctacatgtt cctgtctgac
     9001 attgacttcc tgcccatgta tgggctctat gagtacctca ggaagtctgt catccagctc
     9061 gatcttgcca acaccaagaa agcaatgatt gtccccgcgt tcgagacact gcgctaccgg
     9121 ctgtccttcc ccaagtcaaa agcggagttg ctgtcaatgc tggacatggg gaccctcttc
     9181 acattcaggt accacgtctg gacgaaaggc cacgcaccca caaacttcgc caagtggcgg
     9241 accgccacca cgccttaccg ggttgagtgg gaggccgatt ttgagccgta tgttgttgtg
     9301 agacgtgact gcccggagta cgaccggagg tttgtaggct ttggctggaa caaagtggct
     9361 catatcatgg agctggatgt gcaggagtat gagttcattg tgctgcccaa cgcctacatg
     9421 atccacatgc ctcatgcccc cagcttcgac attaccaagt tccgttccaa caagcaatac
     9481 cgcatctgtc tcaaaaccct caaggaagag tttcagcagg acatgtcccg ccgctacggc
     9541 tttgctgccc tgaaatatct cacagccgag aacaacagct agggatcccc cgaaaagtgc
     9601 cacctgacgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     9661 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
     9721 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     9781 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     9841 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     9901 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     9961 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
    10021 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
    10081 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
    10141 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
    10201 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
    10261 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
    10321 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
    10381 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
    10441 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
    10501 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
    10561 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
    10621 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
    10681 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
    10741 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgacgt acggccacca
    10801 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
    10861 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
    10921 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
    10981 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
    11041 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
    11101 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
    11161 gtgaattgct gccctctggt tatgtgtggg agggctaaga attcgatatc aagcttatcg
    11221 gtaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    11281 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    11341 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    11401 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    11461 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    11521 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    11581 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    11641 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    11701 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    11761 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cggtaccttt
    11821 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
    11881 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
    11941 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
    12001 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
    12061 caatgaagga gagaacaccc gctt
//
