LOCUS       sequence-405997-        3586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for terbinafine resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB414
FEATURES             Location/Qualifiers
     source          1..3586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..99)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(120..779)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     694..713
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     941..959
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(963..1006)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1009..1030
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2568..2625
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2649..2666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2820..3408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2900..2919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
       61 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      121 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
      181 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      241 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      301 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
      361 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      421 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      481 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      541 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      601 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      661 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      721 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      781 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      841 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      961 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     1021 gcttctagag cgatgagtgg tctcgaatga ccttgctcaa tggccacgcc tccctctccc
     1081 cggccgctca acgccgcatc gaacaccatg aagctgatat tgtaattgtg ggtgccggag
     1141 tcctcggctg tgcacttgca gttgcactgg gaaatcaagg gcgcagtgta atcctgctgg
     1201 agaagtcgtt ggaagagccc aaccgtattg ttggtgaact actccaaccg ggaggtgttc
     1261 aggcgctcga gcaactaggg ctacgggatt gtctggaaga tatcgatggc atcaatgtga
     1321 agggatactg ggttacttac tttggggagc cggttctcct cgaatacccc aagtccagcc
     1381 caacctcgcc tacaccgttg ggacgagcct tccaccatgg ccgattcgtg atgaaactac
     1441 gggcagccgc tctgtcgtgc cccaatgtca cggtggtcga aaccaaggtt accggcctca
     1501 tcacatcctc atatactcag gaagtccttg gagtcgagtg tataaccaag gatgtcaagg
     1561 actgctactt cggtcaactc accgtggccg ccgacggtta taactccgtt ttccgcaagg
     1621 aacaccacca gtatacaccc aaacggcgct caaagttcca tggcttggag ctcatcgatg
     1681 ctaaactgcc cgctcccaac actggtcatg tgcttctcag cgataatcca cccgtactta
     1741 tgtaccagat cggcacccat gaaacccgaa tccttatcga tatccctgat aacctgcctg
     1801 cggcctcggt caagaacggt ggtgtcaagg gttacatgcg aaacaacatc ctgcccaagc
     1861 tcccagaggg tgtccagcag tcgttctccg atgccctaga gaagggccag ctacggtcaa
     1921 tgccgaactc gttcctgcct gcctcggcca acaagacacc cggcttgatg attctgggcg
     1981 atgcgcttaa catgcggcac cctctcacag gtggaggcat gactgtggcg ctcaacgatg
     2041 tttgtgtcat ccgcgaattg ctcagcccag agcgcgtgcc caacctctcc aatacaggcc
     2101 ttgttctcga gcagctcgca gagttccact ggaaacgaaa gaactcatct tcggtcatca
     2161 atatcctcgc acaggctctc tatgcgctct tcgctgcaga cagtacgcac gctcactctt
     2221 caggttaatt tgcaataact aactgttcat tcaacagact attacctcaa agctcttcag
     2281 cgtggctgtt tccgctactt ccagattgga ccagttggcg gccctgtcgg gttgttagcc
     2341 ggtctaatca agaaacccct cgtccttgtc agccacttct tctccgttgc cttcctcgcc
     2401 atctgggtcc acatatgcga tacccctctg tccaagctat tcctagcccc atactacgcc
     2461 gtcatgatat tgtacacagc ctctgtcgtg attttgccat acatctggac cgagatctgg
     2521 tactgagctt tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag
     2581 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     2641 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2821 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3541 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggc
//
