LOCUS       sequence-405998-       11115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpress DAG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11115)
  TITLE     Using Saturation Mutagenesis-Reinforced Functional Assays (SMuRF) to
            improve the variant interpretation for alpha-dystroglycan 
            glycosylation enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lenti-DAG1
FEATURES             Location/Qualifiers
     source          1..11115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1990..2201
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4920..4976
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4977..5573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5470..5490
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5589..6177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5642..5662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6060..6071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6249..6482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6508..6525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6514..6738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6784..7212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6871..6890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7081..7102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7221..7241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7226..7555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7406..7541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7468..7487
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7603..7650
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7669..8043
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8173..8294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8210..8229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8264..8283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8343..8359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8356..8378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8391..8397,8398..8415,8416..8421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8436..8457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8574..8591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8745..9333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8825..8844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9504..10295,10296..10364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10127..10146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10365..10469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10544..10563)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10735..11114
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2041 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2101 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2161 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgctg
     2221 ccaccatgag gatgtctgtg ggcctctcgc tgctgctgcc cctctggggg aggacctttc
     2281 tcctcctgct ctctgtggtt atggctcagt cccactggcc cagtgaaccc tcagaggctg
     2341 tcagggactg ggaaaaccag cttgaggcat ccatgcactc agtgctctca gacctccacg
     2401 aggctgttcc cacagtggtt ggcattcctg atggcacggc tgtcgtcggg cgctcatttc
     2461 gagtgaccat tccaacagat ttgattgcct ccagtggaga tatcatcaag gtatcagcgg
     2521 cagggaagga ggctttgcca tcttggctgc actgggactc acagagccac accctggagg
     2581 gcctccccct tgacactgat aagggtgtgc attacatttc agtgagcgct acacggctgg
     2641 gggccaacgg gagccacatc ccccagacct ccagtgtgtt ctccatcgag gtctaccctg
     2701 aagaccacag tgagctgcag tcggtgagga cagcctcccc agaccctggt gaggtggtat
     2761 catctgcctg tgctgcggat gaacctgtga ctgttttgac ggtgattttg gatgccgacc
     2821 tcaccaagat gaccccaaag caaaggattg acctcctgca caggatgcgg agcttctcag
     2881 aagtagagct tcacaacatg aaattagtgc cggtggtgaa taacagacta tttgacatgt
     2941 cggccttcat ggctggcccg ggaaatgcaa aaaaggtggt ggagaatggg gcccttctct
     3001 cctggaagct gggctgctcc ctgaaccaga acagtgtgcc tgacattcat ggtgtagagg
     3061 cccctgccag ggagggcgca atgtctgctc agcttggcta ccctgtggtg ggttggcaca
     3121 tcgccaataa gaagccccct cttcccaaac gcgtccggag gcagatccat gctacaccca
     3181 cacctgtcac tgccattggg cccccaacca cggctatcca ggagccccca tccaggatcg
     3241 tgccaacccc cacatctcca gccattgctc ctccaacaga gaccatggct cctccagtca
     3301 gggatcctgt tcctgggaaa cccacggtca ccatccggac tcgaggcgcc attattcaaa
     3361 ccccaaccct aggccccatc cagcctactc gggtgtcaga agctggcacc acagttcctg
     3421 gccagattcg cccaacgatg accattcctg gctatgtgga gcctactgca gttgctaccc
     3481 ctcccacaac caccaccaag aagccacgag tatccacacc aaaaccagca acgccttcaa
     3541 ctgactccac caccaccacg actcgcaggc caaccaagaa accacggaca ccccggccag
     3601 tgccccgggt caccaccaaa gtttccatca ccagattgga aactgcctca ccgcctactc
     3661 gtattcgcac caccaccagt ggagtgcccc gtggcggaga acccaaccag cgcccagagc
     3721 tcaagaacca tattgacagg gtagatgcct gggttggcac ctactttgag gtgaagatcc
     3781 cgtcagacac tttctatgac catgaggaca ccaccactga caagctgaag ctgaccctga
     3841 aactgcggga gcagcagctg gtgggcgaga agtcctgggt acagttcaac agcaacagcc
     3901 agctcatgta tggccttccc gacagcagcc acgtgggcaa acacgagtat ttcatgcatg
     3961 ccacagacaa ggggggcctg tcggctgtgg atgccttcga gatccacgtc cacaggcgcc
     4021 cccaagggga tagggctcct gcaaggttca aggccaagtt tgtgggtgac ccggcactgg
     4081 tgttgaatga catccacaag aagattgcct tggtaaagaa actggccttc gcctttggag
     4141 accgaaactg tagcaccatc accctgcaga atatcacccg gggctccatc gtggtggaat
     4201 ggaccaacaa cacactgccc ttggagccct gccccaagga gcagatcgct gggctgagcc
     4261 gccggatcgc tgaggatgat ggaaaacctc ggcctgcctt ctccaacgcc ctagagcctg
     4321 actttaaggc cacaagcatc actgtgacgg gctctggcag ttgtcggcac ctacagttta
     4381 tccctgtggt accacccagg agagtgccct cagaggcgcc gcccacagaa gtgcctgaca
     4441 gggaccctga gaagagcagt gaggatgatg tctacctgca tacagtcatt ccggccgtgg
     4501 tggtcgcagc catcctgctc attgctggca tcattgccat gatctgctac cgcaagaagc
     4561 ggaagggcaa gcttaccctt gaggaccagg ccaccttcat caagaagggg gtgcctatca
     4621 tctttgcaga cgaactggac gactccaagc ccccaccctc ctccagcatg ccactcattc
     4681 tgcaggagga gaaggctccc ctaccccctc ctgagtaccc caaccagagt gtgcccgaga
     4741 ccactcctct gaaccaggac accatgggag agtacacgcc cctgcgggat gaggatccca
     4801 atgcgcctcc ctaccagccc ccaccgccct tcacagcacc catggagggc aagggctccc
     4861 gtcccaagaa catgacccca taccggtcac ctcctcccta tgtcccacct ggatccggcg
     4921 caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct ggaccgaccg
     4981 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5041 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5101 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5161 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5221 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5281 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5341 ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5401 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5461 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5521 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt
     5581 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5641 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5701 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5761 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5821 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5881 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5941 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6001 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6061 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6121 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     6181 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6241 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6301 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6361 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6421 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6481 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6541 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6601 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6661 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6721 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6781 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6841 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6961 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7021 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7081 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7141 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7201 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7321 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7381 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7561 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7621 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7681 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7741 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7801 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7861 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7921 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7981 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8041 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8161 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10501 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    10561 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    10621 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    10681 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    10741 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    10801 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    10861 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    10921 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    10981 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    11041 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    11101 tcgctattac catgg
//
