LOCUS       sequence-406136-       10530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Turkki P, Chowdhury I, Ohman T, Azizi L, Varjosalo M, Hytonen VP
  TITLE     Tensin-2 interactomics reveals interaction with GAPDH and a 
            phosphorylation-mediated regulatory role in glycolysis.
  JOURNAL   Sci Rep. 2024 Aug 27;14(1):19862. doi: 10.1038/s41598-024-65787-7.
  PUBMED    39191795
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-08-27"; volume: "14(1):19862. doi"; pages: " 
            10.1038/s41598-024-65787-7"
COMMENT     Sequence Label: Tensin-2 Y483F
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7222..7246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7293..7316
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7353..7376
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7407..7433
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7407..7433
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7434..7781,7782..7784,7785..8393)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8437..8454)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8443..8667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8800..8819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8951..8998
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9006..10028
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9006..10028
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10158..10279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10195..10214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10249..10268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10328..10344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10328..10344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10341..10363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10352..10368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10376..10382,10383..10400,10401..10406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10421..10442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagc aggctccacc atgaagtcca gcggccctgt ggaaaggttg ctcagagccc
     3061 tggggaggag ggacagcagc cgggccacca gcaggccgag gaaagcagaa ccacacagct
     3121 tccgggagaa ggttttccgg aagaaaactc cagtgtgtgc agtgtgtaag gtgaccatcg
     3181 atgggaccgg tgtctcctgc cgagtctgca aggtggccac acacagaaaa tgtgaagcaa
     3241 aggtgacttc atcctgtcag gccttgcccc ctgcggagct gcggagaagc acagccccgg
     3301 tccggcgcat agaacacctg gggtccacca agtctctgaa ccactcaaag caacgcagta
     3361 ctctacccag gagcttcagc ctagatccgc tcatggagcg tcgctgggac ttggacctca
     3421 cctatgtaac ggagcggatc ttggccgcag cttttcctgc acgaccggac gagcagcgac
     3481 accgaggcca cttgcgcgag ctagcccatg tgcttcaatc caagcacaga gacaagtacc
     3541 tgctcttcaa cctttcagag aaacggcatg acctcacccg cctgaacccc aaggtgcaag
     3601 actttggttg gcctgaacta catgccccac ctctggacaa gttgtgttcc atctgcaaag
     3661 ccatggagac gtggctcagt gccgaccctc agcacgtggt tgtgttgtac tgcaagggga
     3721 gcaaaggcaa gcttggggtc atcgtctctg cttatatgca ctacagcaag atctctgcag
     3781 gggcagacca ggcgctggca accctcacca tgaggaaatt ctgtgaggac aaggtggcca
     3841 cggaactgca gccctcccag cgccgctatg tcagctactt cagcggactg ctgtctggct
     3901 ccatcagaat gaacagcagc ccactcttcc tgcactatgt gtttgttcct gtgctgccag
     3961 cctttgagcc caacacaggc ttccagccct tcctcaagat ctaccagtct atgcagctgg
     4021 tctacacatc tggagtctac cgcatagcag gtccagggcc tcagcaactt tgtatcagcc
     4081 tggagccagc ccttctcctc aaaggcgacg tcatggtaac ctgctaccac aagggtggcc
     4141 aggggacaga ccggaccctc gtgttccgag ttcagttcca cacatgcacc attcatggat
     4201 cacggctcac cttcccaaag gaccaactag atgaggcctg ggctgatgag cggtttcctt
     4261 tccaagcctc agtggagttt gttttctcct ccagccctga gaaggtcaaa ggcaacaccc
     4321 cacggaatga tccctctgtc tccgtggatt acaacacgac agagcctgct gtacgctggg
     4381 actcctatga aaacttcaat cagcaccatg aggatagcgt ggacggcgcc ttggcccaca
     4441 caaggggccc cctggatggc agtcctttcg cccaggtgca gcgggtcccc cgccagacac
     4501 caccagcacc ttctccagag cttcccccac ccccgatgct ctctgtcagc agcgactctg
     4561 gccactcatc cacgctaacc acagaacaca cagcagaatc ccccggccgg ccgcccccaa
     4621 ctgctgctga aagacaggag ctggatcgtc tgctgggagg ttgtggagtg gccagtgcgg
     4681 gccgtggtgc tgggcgagag accgccatcc ttgatgatga agagcagccc tctgtgggtg
     4741 gaggcctgca ccttgggatg tattcaggcc acaggcctgg actcagtcga cgctgctcct
     4801 gccgtcaggg cttccgggag ccttgtgggg tccctaatgg gagctactac cggcctgagg
     4861 gaaccctaga gagacgacga ccaccctatg ggggctatga gggacacccc cagggctacg
     4921 cagaagcctc tgtggagaag agacgcctct gcaggtcact gtcagaaggg ccgtacccct
     4981 atgcacccga gctggggaaa ccagccaatg gggactttgg ctaccgtcca gcaggctacc
     5041 gggaggtggt gatcctggag gaccctgggg tgcctgcttt atgctcatgc cctgcctgtg
     5101 aagagaagct ggcactgccc accgcagccc tgtatggact gcgactagag agggaggctg
     5161 cagaggggtg gtccagcgag gtaggcaagc ctctcctgca cccagtgagg cctggacacc
     5221 cattgcctct gctggtgcct gcctgtgggc atcatcatgc cccaatgcct gactatggct
     5281 gcctgaagcc acccaaggtg ggtgaggaag ggcatgaggg ctgctcctat gccgtgtgct
     5341 ctgaaggcag gtatgggcat tcagggtacc ctgccctggt aacctatggc tatggaggag
     5401 cggttcccag ctactgccca gcatatggcc gggcacctca cagttgcgga tctccaagtg
     5461 aaggccgagg gtaccccagc cctggtgccc actcaccacg ggctggatct gtgtccccgg
     5521 ggagtccacc ctacctgcag cccaggaagc tgggttatga gatttctgcg gaggatggga
     5581 gagacaagta cccactttct ggacacctgg cctcaacagg acccttggct tctacagagt
     5641 cacctgaacc atcctggagg gatggctcca gtggacacag cacgctacct cggtctcccc
     5701 gagatcctca gtgcagtgct tcttcagagc tgtctggtcc ctccacaccc ttacacacca
     5761 gcagcccagt tcagggcaag gaaagcaacc gacggcaaga caccaccagg tctccctcct
     5821 tggcacccac tcagagactg agtcctggcg aggccttgcc ctctgttgta cagggagttg
     5881 ctgaaaagac tcctgagctg ttgacaagca gcaggcctga gcaactggac cctagcccct
     5941 tctcccagac ctctgcaccc ggctcaccca atggctggcc tcaggaaagg agcccaggag
     6001 gccacaccaa cagtgctagt cctcggagcc ctgtgccaac caccctgcct ggacttcgcc
     6061 atgccccgtg gcagggccct cggggcactt cagatagccc agatggctcc cctcttactc
     6121 ctgtgcctac ccagatgccc tggcttgtgg gcagcccaga accaccccag agctcaccca
     6181 cccctgcctt ccccctggct acctcctatg atgccaatgg tcccattcag cctccacttc
     6241 ctgaaaaacg acacctgcct gggtctgggc aacagccgtc gccaccagcc agaagcacca
     6301 atcaacatgt cacctttgca tctcctctcc cagatgtcac ccaaccccca gaacaccctt
     6361 tacaagagaa ccaaagcaat gtcaagtttg tccaggatac atcaaagttc tggtacaagc
     6421 cacatctttc ccgagaccaa gccattgccc tgctgaaaga caaggatcct ggggccttcc
     6481 tgattagaga cagccattca ttccaaggag cctatgggct agccctcaag gtggctacac
     6541 ctccacccag tgcgcagccc tggaaagggg acccctcaga acagctggtc cgccatttcc
     6601 tcatcgagac tgggcccaaa ggcgtgaaga tcaagggttg tcccactgag ccctattttg
     6661 gaagcctgtc tgccctggtc tcccagcact ccatctctcc catctccctg ccctgctgtc
     6721 tgagaattcc tagcaaagat cctctagaag aaaccccaga ggccccagtg cctaccaaca
     6781 tgagcacagc agcagacctc ctgcgtcaag gagcagcctg cagtgtgctc tacctgacct
     6841 cggtggagac agagtcattg actggccctc aggctgtggc caaggccagc tctgcagctc
     6901 tgagctgcag cccggtccca gtgccagcca ttgtccattt caaggtctca gctcaaggca
     6961 tcacactgac agacaaccag agaaagctct tctttcgccg ccattaccca gtgaacagca
     7021 tcaccttctc tagcactgac ccccaggacc gcagatggac caacccagat ggagccacct
     7081 ccaagatctt tggtttcgtg gccaagaagc caggaagtcc ctgggaaaat gtgtgtcacc
     7141 tctttgcgga gcttgacccg gaccaaccgg caagtgccat tgtcaccttc atcaccaaag
     7201 ttctactggg ccagaggaaa gacccagctt tcttgtacaa agtggttgat atcaaggccg
     7261 ccgacatcac cagcctgtac aagaaagtgg ggtggagcca tccccagttc gagaagggcg
     7321 gaggatctgg cggcggaagc ggcggaggaa gctggtccca ccctcagttt gagaaaggca
     7381 ccgagctggg cagcaccatg gccagctacc cctacgacgt gcccgactac gccaaggaca
     7441 acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc
     7501 agctgggcga gacactgggc atgagcagag ccgccatcaa caagcacatc cagaccctgc
     7561 gggactgggg cgtggacgtg ttcacagtgc ccggcaaggg ctacagcctg cccgagccca
     7621 tccagctgct gaacgccaag cagatcctgg gccagctgga tggcggatct gtggccgtgc
     7681 tgcccgtgat cgacagcacc aaccagtatc tgctcgaccg gatcggcgag ctgaagtccg
     7741 gcgacgcctg tatcgccgag taccagcagg ccggcagagg cggcaggggc agaaagtggt
     7801 tcagcccctt cggcgccaac ctgtacctga gcatgttctg gagactggaa cagggcccag
     7861 ccgccgctat tggcctgagc ctggtcatcg gcatcgtgat ggccgaggtg ctgcggaagc
     7921 tgggcgccga caaagtgcgc gtgaagtggc ccaacgacct gtatctgcag gaccggaagc
     7981 tggccggcat cctggtggaa ctgaccggca aaacaggcga cgccgcccag atcgtgatcg
     8041 gcgctggcat caacatggcc atgcggcggg tggaagagag cgtggtcaac cagggctgga
     8101 tcaccctgca ggaagccggc atcaacctgg accggaacac cctggccgcc atgctgatca
     8161 gagagctgag agccgccctg gaactgttcg agcaggaagg cctggccccc tacctgagca
     8221 gatgggagaa gctggacaac ttcatcaacc ggcccgtgaa actgatcatc ggcgacaaag
     8281 agatcttcgg catcagccgg ggcatcgaca agcagggggc cctgctcctg gaacaggacg
     8341 gcatcatcaa gccctggatg ggcggcgaga tcagcctgcg gagcgccgag aagtgatgac
     8401 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8461 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8521 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8581 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8641 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8701 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8761 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8821 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8881 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8941 cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct
     9001 tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     9061 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     9121 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     9181 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     9241 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     9301 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     9361 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     9421 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     9481 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     9541 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     9601 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     9661 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     9721 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     9781 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     9841 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     9901 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     9961 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
    10021 aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    10081 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    10141 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10261 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
    10321 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10381 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10441 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10501 aatgaatcgg ccaacgcgcg gggagaggcg
//
