LOCUS       sequence-406175-       12462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flippase under heat shock control. HDR integrant marked with 
            ie1-EGFP, expressed in abdomen and mouthparts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 12462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT75
FEATURES             Location/Qualifiers
     source          1..12462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1220..1343)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1435..1454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1436..1454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1907..2698
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2944..3532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3686..3703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3735..3753
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3735..3752
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        3830..4250
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    4257..4380
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4403..4408,4409..4426,4427..4433)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        4476..4580
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4581..4649,4650..5441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4799..4818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5962..5981
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6121..6244)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        6281..6763
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        6767..7358
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     regulatory      7543..7552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7549..7551,7552..7554,7555..8268)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7549..7551,7552..7554,7555..8265)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7594..7615)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7855..7874)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8202..8223
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8391..8512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8428..8447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8482..8501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(9061..10332)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        complement(10388..10626)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(10420..10441)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    10878..11001
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(11024..11029,11030..11047,11048..11054)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             11108..11767
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11174..11193)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        11776..12221
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(12222..12308,12309..12462,1..1181)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 gcctggacgt ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc
       61 acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga
      121 tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc
      181 gcgtctttaa agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac
      241 aagaatggtc aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg
      301 atttctccgg taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag
      361 catcagacag ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg
      421 acggaaaaac gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg
      481 acaaccatac gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat
      541 ttgaagcaaa cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag
      601 catactatgg caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg
      661 ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg
      721 attacacact gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa
      781 ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg
      841 gatcaaaaat gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt
      901 ctaattcttt aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg
      961 atcttgatcc taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag
     1021 gaaacaatgt cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat
     1081 caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag
     1141 acagcatcct tgaacaagga caattaacag ttaacaaata aatgtcaggc tcccttatac
     1201 acagccagtc tgcacgtcgc atagtgactg gatatgttgt gttttacagt attatgtagt
     1261 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     1321 ttcaactttt taatacaaag ttgcctccgg aacataatgg tgcatctaga tgcccattcc
     1381 tgtagacttc ttaattaaga cgtcagaatt ctcgaggcgg ccgcatgtga gtctccctat
     1441 agtgagtcgt attaatttcg cgggcgcgtc agcgggagag ccgttgtaag gcggcagact
     1501 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     1561 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     1621 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     1681 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1741 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     1801 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1861 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     1921 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1981 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2041 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2101 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2161 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2221 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2281 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2341 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2401 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2461 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2521 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2581 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     2641 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     2701 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     2761 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     2821 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     2881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3601 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3661 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaaatcg tcacctgcag
     3721 gaaggtttaa acgcatttag gtgacactat agaagtgtgt atcgctcgag ggatccgaat
     3781 tcgaagtctt ggtacggagg ggatccgatc gacaagccga acatatgggt tttttgctca
     3841 cctgtgattg ctcctactca aatacaaaaa catcaaattt tctgtcaata aagcatattt
     3901 atttatattt attttacagg aaagaattcc ttttaaagtg tattttaacc tataatgaaa
     3961 aacgattaaa aaaaatacat aaaataattc gaaaattttt gaatagccca ggttgataaa
     4021 aattcatttc atacgtttta taacttatgc ccctaagtat tttttgacca tagtgtttca
     4081 attctacatt aattttacag agtagaatga aacgccacct actcagccaa gaggcgaaaa
     4141 ggttagctcg ccaagcagag agggcgccag tgctcactac tttttataat tctcaacttc
     4201 tttttccaga ctcagttcgt atatatagac ctattttcaa tttaacgtcg gggcctcaac
     4261 tttgtataat aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     4321 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     4381 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
     4441 tttgagttag gatccgtcga gattttcagg agctacgcgg aacccctatt tgtttatttt
     4501 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4561 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4621 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4681 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4741 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4801 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4861 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4921 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4981 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5041 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5101 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5161 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5221 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5281 gagccggtga gcgtgggtcc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5341 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5401 agatcgctga gataggtgcc tcactgatta agcattggta atggatccgg cttactaaaa
     5461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     5521 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     5581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     5641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     5701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     5761 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     5821 gtctgtttgt ggatgtacag agtgatatta tcgatacacc gggccgtcgc atggttattc
     5881 cacttgcgtc tgcgcgcttg ctctcagata aagtctcccg tgaactttac ccggtggtgc
     5941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtttccg
     6001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     6061 acctgatgtt ctggggaata taatgtcagg ctcccttata cacagccagt ctgcacgtcg
     6121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     6181 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa
     6241 gttgtccagc tcttcctact gcaccgagct cttacgcgcc cgcgtaaaac acaatcaagt
     6301 atgagtcata agctgatgtc atgttttgca cacggctcat aaccgaactg gctttacgag
     6361 tagaattcta cttgtaacgc acgatcagtg gatgatgtca tttgtttttc aaatcgagat
     6421 gatgtcatgt tttgcacacg gctcataaac tcgctttacg agtagaattc tacgtgtaac
     6481 gcacgatcga ttgatgagtc atttgttttg caatatgata tcatacaata tgactcattt
     6541 gtttttcaaa accgaacttg atttacgggt agaattctac ttgtaaagca caatcaaaaa
     6601 gatgatgtca tttgtttttc aaaactgaac tcgctttacg agtagaattc tacgtgtaaa
     6661 acacaatcaa gaaatgatgt catttgttat aaaaataaaa gctgatgtca tgttttgcac
     6721 atggctcata actaaactcg ctttacgggt agaattctac gcgcgtcgat gtctttgtga
     6781 tgcgcgcgac atttttgtag gttattgata aaatgaacgg atacgttgcc cgacattatc
     6841 attaaatcct tggcgtagaa tttgtcgggt ccattgtccg tgtgcgctag catgcccgta
     6901 acggacctcg tacttttggc ttcaaaggtt ttgcgcacag acaaaatgtg ccacacttgc
     6961 agctctgcat gtgtgcgcgt taccacaaat cccaacggcg cagtgtactt gttgtatgca
     7021 aataaatctc gataaaggcg cggcgcgcga atgcagctga tcacgtacgc tcctcgtgtt
     7081 ccgttcaagg acggtgttat cgacctcaga ttaatgttta tcggccgact gttttcgtat
     7141 ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg gcggatgttc tatatctaat
     7201 ttgaataaat aaacgataac cgcgttggtt ttagagggca taataaaaga aatattgtta
     7261 tcgtgttcgc cattagggca gtataaattg acgttcatgt tggatattgt ttcagttgca
     7321 agttgacact ggcggcgaca agatcgtgaa caaccaagtg accctcgacc gccggagtat
     7381 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     7441 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatcggggta
     7501 ccgctagagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     7561 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     7621 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     7681 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     7741 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     7801 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     7861 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     7921 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7981 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     8041 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8101 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     8161 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8221 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     8281 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8341 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaagcttat
     8521 cgatacgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     8581 aggtcgcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     8641 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagcg gccgcgttta
     8701 aacaccggtt agggatgcca actcgtgctt ttaagcgcgt aaagttttcg attcggcgcc
     8761 aactctccat cgcatttaaa taaataattt ctcgaggaca gatctaaacg agtttttaag
     8821 caaactcact ccctgacaat aaaaacgcaa ttatctttaa ataaatatat tctatttatt
     8881 aaccaagtag ctaatatagc taaaatcaat ttgttgctaa cttaaaacta acataattat
     8941 gtattagtta tttacattct tttggttggt tacaaccaaa taaataatat cacttttaaa
     9001 aacttaagcc aggaactgaa taattttcta tttggcttta gtcgacccta gggtttaaac
     9061 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     9121 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     9181 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     9241 atatgcatag taccgagaaa ctagtgcgaa gtagtgatca ggtattgctg ttatctgatg
     9301 agtatacgtt gtcctggcca cggcagaagc acgcttatcg ctccaatttc ccacaacatt
     9361 agtcaactcc gttaggccct tcattgaaag aaatgaggtc atcaaatgtc ttccaatgtg
     9421 agattttggg ccatttttta tagcaaagat tgaataaggc gcatttttct tcaaagcttt
     9481 attgtacgat ctgactaagt tatcttttaa taattggtat tcctgtttat tgcttgaaga
     9541 attgccggtc ctatttactc gttttaggac tggttcagaa ttcctcaaaa attcatccaa
     9601 atatacaagt ggatcgatcc taccccttgc gctaaagaag tatatgtgcc tactaacgct
     9661 tgtctttgtc tctgtcacta aacactggat tattactccc agatacttat tttggactaa
     9721 tttaaatgat ttcggatcaa cgttcttaat atcgctgaat cttccacaat tgatgaaagt
     9781 agctaggaag aggaattggt ataaagtttt tgtttttgta aatctcgaag tatactcaaa
     9841 cgaatttagt attttctcag tgatctccca gatgctttca ccctcactta gaagtgcttt
     9901 aagcattttt ttactgtggc tatttccctt atctgcttct tccgatgatt cgaactgtaa
     9961 ttgcaaacta cttacaatat cagtgatatc agattgatgt ttttgtccat agtaaggaat
    10021 aattgtaaat tcccaagcag gaatcaattt ctttaatgag gcttccagaa ttgttgcttt
    10081 ttgcgtcttg tatttaaact ggagtgattt attgacaata tcgaaactca gcgaattgct
    10141 tatgatagta ttatagctca tgaatgtggc tctcttgatt gctgttccgt tatgtgtaat
    10201 catccaacat aaataggtta gttcagcagc acataatgct attttctcac ctgaaggtct
    10261 ttcaaacctt tccacaaact gacgaacaag caccttaggt ggtgttttac ataatatatc
    10321 aaattgtggc attttgattg ctagcggtac ctgagttctt ctttctcggt aacttgttga
    10381 aagtattcag agttctcttc ttgtattcaa taattacttc ttggcagatt tcagtagttg
    10441 cagttgattt acttggttgc tggttacttt taattgattc actttaactt gcactttact
    10501 gcagattgtt tagcttgttc agctgcgctt gtttatttgc ttagctttcg cttagcgacg
    10561 tgttcacttt gcttgtttga attgaattgt cgctccgtag acgaagcgcc tctatttata
    10621 ctccggcgct cttttcgcga acattcgagg cgcgctctct cgaaccaacg agagcagtat
    10681 gtcgtttact gtgtgacaga gtgagagagc attagtgcag agagggagag acccaaaaag
    10741 aaaagagaca ataacgaata acggccagag aaatttctcg agttttcttt ctgccaaaca
    10801 aatgacctac cacaataacc agtttgtttt gggattcaga tctggccggc cgaattcttg
    10861 aagacgaaag ggcctcgcaa ctttgtatac aaaagttgaa cgagaaacgt aaaatgatat
    10921 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
    10981 acaacatatc cagtcactat gggccgcatt aggcacccca ggctttacac tttatgcttc
    11041 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
    11101 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
    11161 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
    11221 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
    11281 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
    11341 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
    11401 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
    11461 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
    11521 tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca gttttgattt
    11581 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
    11641 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
    11701 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
    11761 ggcgtaaacg cgtggcacat atacctgccg ttcactatta tttagtgaaa tgagatatta
    11821 tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa
    11881 aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc
    11941 aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac
    12001 gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact gataaagcag
    12061 gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc ttacatattt
    12121 taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg gctggaagaa
    12181 gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc aaaaagtttg
    12241 caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag
    12301 cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta
    12361 cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagttcctg
    12421 aattcgattc gtccacaatt aaaaatatct cttctgcaaa ag
//
