LOCUS       sequence-406176-       15088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For introducing Cas9 under D. melanogaster nanos promoter. Markers 
            with EYFP expressed in eyes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT17
FEATURES             Location/Qualifiers
     source          1..15088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..44)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        627..1072
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1073..1159,1160..2494)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(2533..2656)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2748..2767)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2749..2767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3220..4011
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4257..4845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4746..4765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4999..5016
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5048..5066
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5048..5065
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        5143..5563
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    5570..5693
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5716..5721,5722..5739,5740..5746)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        5789..5893
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5894..5962,5963..6754)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6112..6131)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7275..7294
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7434..7557)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8500..12603
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8500..12603
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8500..12603
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12616..12636
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13582..13703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13593..13612)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13647..13666
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(13826..14539,14540..14542,14543..14545))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13871..13892)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     14220..14239
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14479..14500
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      14542..14551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    14817..14940
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(14963..14968,14969..14986,14987..14993)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(15047..15088,1..618)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
       61 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      121 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
      181 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      241 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      301 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
      361 cctaaagggt ttattgagaa tatgtttttc gtgtcagcca atccctgggt gagtttcacc
      421 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
      481 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
      541 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
      601 tggcagggcg gggcgtaaac gcgtggcaca tatacctgcc gttcactatt atttagtgaa
      661 atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg cccatgcaac
      721 agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag ttctttaggc
      781 ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa tagaccagtt
      841 gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg ggtttgttac
      901 tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt ggcgtcaccc
      961 cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt caaacaggag
     1021 ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa cgatgaacat
     1081 caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc tggcaggagg
     1141 cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa catacggcat
     1201 ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa atgaaaaata
     1261 tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa aaggcctgga
     1321 cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact atcacggcta
     1381 ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat cgatttacat
     1441 gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg gccgcgtctt
     1501 taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa cacaagaatg
     1561 gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca ctgatttctc
     1621 cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat cagcatcaga
     1681 cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg gtgacggaaa
     1741 aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag gcgacaacca
     1801 tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag tatttgaagc
     1861 aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca aagcatacta
     1921 tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa
     1981 acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg atgattacac
     2041 actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg aaattgaacg
     2101 cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc gcggatcaaa
     2161 aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg tttctaattc
     2221 tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa tggatcttga
     2281 tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga aaggaaacaa
     2341 tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac aatcaacgtt
     2401 tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca aagacagcat
     2461 ccttgaacaa ggacaattaa cagttaacaa ataaatgtca ggctccctta tacacagcca
     2521 gtctgcacgt cgcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     2581 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
     2641 ttttaataca aagttgcctc cggaacataa tggtgcatct agatgcccat tcctgtagac
     2701 ttcttaatta agacgtcaga attctcgagg cggccgcatg tgagtctccc tatagtgagt
     2761 cgtattaatt tcgcgggcgc gtcagcggga gagccgttgt aaggcggcag actttgctca
     2821 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     2881 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     2941 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     3001 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     3061 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     3121 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     3181 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     3241 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3301 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     3361 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     3421 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     3481 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     3541 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     3601 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     3661 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     3721 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     3781 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3841 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3901 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     3961 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     4021 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     4081 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     4141 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     4201 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4261 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4321 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4381 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4441 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4501 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4561 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4621 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4681 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4741 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4801 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4861 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4921 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4981 agccgaacga ccgagcgcag cgagtcagtg agcgaggaaa tcgtcacctg caggaaggtt
     5041 taaacgcatt taggtgacac tatagaagtg tgtatcgctc gagggatccg aattcgaagt
     5101 cttggtacgg aggggatccg atcgacaagc cgaacatatg ggttttttgc tcacctgtga
     5161 ttgctcctac tcaaatacaa aaacatcaaa ttttctgtca ataaagcata tttatttata
     5221 tttattttac aggaaagaat tccttttaaa gtgtatttta acctataatg aaaaacgatt
     5281 aaaaaaaata cataaaataa ttcgaaaatt tttgaatagc ccaggttgat aaaaattcat
     5341 ttcatacgtt ttataactta tgcccctaag tattttttga ccatagtgtt tcaattctac
     5401 attaatttta cagagtagaa tgaaacgcca cctactcagc caagaggcga aaaggttagc
     5461 tcgccaagca gagagggcgc cagtgctcac tactttttat aattctcaac ttctttttcc
     5521 agactcagtt cgtatatata gacctatttt caatttaacg tcggggcctc aactttgtat
     5581 aataaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     5641 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atgggccgca
     5701 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     5761 taggatccgt cgagattttc aggagctacg cggaacccct atttgtttat ttttctaaat
     5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6601 tgagcgtggg tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6721 tgagataggt gcctcactga ttaagcattg gtaatggatc cggcttacta aaagccagat
     6781 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     6841 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     6901 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     6961 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     7021 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     7081 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     7141 tgtggatgta cagagtgata ttatcgatac accgggccgt cgcatggtta ttccacttgc
     7201 gtctgcgcgc ttgctctcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     7261 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt ccgttatcgg
     7321 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     7381 gttctgggga atataatgtc aggctccctt atacacagcc agtctgcacg tcgcatagtg
     7441 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     7501 atatattgat atttatatca ttttacgttt ctcgttcaac ttttctatac aaagttgtcc
     7561 agctaagctt cgaccgtttt aacctcgaaa tatgcacatg taaggacgga tgtgagcgaa
     7621 cgccagtgat gaccgggatc agaggtaacc taccatgcat ggtggggatt aggtgaccgt
     7681 tcgcaggtag tttgatcgga gcgaatgttc ggggggtctg gcgtcagagg ctctaaactt
     7741 tatgtaattc ctgccgcgaa acacgcacgt atcaagcagt cagctgttct cttcgttcag
     7801 cgcgcgccgg tgttgcaaaa cgagcgctct tcgccggcgg tggctcgtgc gatagttcgt
     7861 tttgtcggta atccgatgtt gccgcgccga tatcatgtga tgttgtcaca gtgcgcgaaa
     7921 ttcgaatggt ggtgtgcagt gattgtgttg tgacggcgag tggcgcgtgt gggtgcttag
     7981 ttttgggaga tgttttcgta tttttttgtt gataactcag gctttgttgc tgtgttgtag
     8041 tactattttc cattgcgcgg tgtccagctt ttaattagtg gcacatattc ttagcaagta
     8101 aaaattattt tgcatactat taaatttctt ataaattatt ttctaaaatt aagtttacct
     8161 tttcaatttt actaaaaata tcgatatatt tattatcgct ggaaaactac attattccac
     8221 ctctaagcaa gaaccgttag ttggcgcgta gctttaccac aaaattcctg gaattgccgt
     8281 acgcttcgca gttgtttcaa gttgtctaag ggacatacga ttttttttgc ctctgcgtca
     8341 cgattttaac ccaaaagcga gtttagttac atgtacatta ttattagata aagaagtatc
     8401 gcgaatactt cagttgaata aactgtgctt ggtttttggg tgaggatttg tggaaagtag
     8461 agtgcgcgat aaccgtaact ttcgacccgg attttcgcca tggacaagaa gtactccatt
     8521 gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
     8581 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
     8641 attggcgccc tcctgttcga ctccggggaa acggccgaag ccacgcggct caaaagaaca
     8701 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
     8761 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
     8821 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
     8881 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
     8941 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
     9001 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
     9061 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
     9121 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
     9181 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
     9241 ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc
     9301 aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca
     9361 gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga tattctgcga
     9421 gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg ctatgatgag
     9481 caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc tgagaagtac
     9541 aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga cggcggagca
     9601 agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga cggcaccgag
     9661 gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac tttcgacaat
     9721 ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag gcggcaagag
     9781 gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct cacatttcgg
     9841 ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc
     9901 aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa gggggcctct
     9961 gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa cgaaaaggtg
    10021 cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct caccaaggtc
    10081 aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca gaagaaagct
    10141 atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct caaagaagac
    10201 tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga ggatcgcttc
    10261 aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg
    10321 gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac gttgtttgaa
    10381 gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga cgacaaagtc
    10441 atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag aaaactgatc
    10501 aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa gtccgatgga
    10561 tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt taaggaggac
    10621 atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat cgctaatctt
    10681 gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc
    10741 gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc ccgagagaac
    10801 caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat tgaagagggt
    10861 ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac ccagcttcag
    10921 aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt ggatcaggaa
    10981 ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca gtcttttctc
    11041 aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag agggaagagt
    11101 gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg gcagctgctg
    11161 aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga acgaggtggc
    11221 ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac acgccagatc
    11281 accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga tgaaaatgac
    11341 aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc agatttcaga
    11401 aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc gcatgatgcc
    11461 tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct tgaatctgaa
    11521 tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag
    11581 gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa ttttttcaag
    11641 accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga aacaaacgga
    11701 gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg gaaggtcctg
    11761 tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg cttctccaag
    11821 gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa agattgggac
    11881 cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact ggttgtggcc
    11941 aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct gggcatcaca
    12001 atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat
    12061 aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt tgagcttgaa
    12121 aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca
    12181 ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa gctcaaaggg
    12241 tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca ctaccttgat
    12301 gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga cgctaacctc
    12361 gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga gcaggcagaa
    12421 aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc
    12481 gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga cgccacactg
    12541 attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca gctcggtgga
    12601 gacagcaggg ctgaccccaa gaagaagagg aaggtgtgag cgaatccagc tctggagcag
    12661 aggctctggc agcttttgca gcgtttatat aacatgaaat atatatacgc attccgatca
    12721 aagctgggtt aaccagatag atagtaacgt ttaactagcg cctggcgcgt tcgattttaa
    12781 agagatttag agcgttatcc cgtgcctata gatcttatag tatagacaac gaacgatcac
    12841 tcaaatccaa gtcaataatt caagaattta tgtctgtttc tgtgaaaggg aaactaattt
    12901 tgttaaagaa gacttacaat atcgtaatac ttgttcaatc gtcgtggccg atagaaatat
    12961 cttacaatcc gaaagttgat gaatggaatt ggtctgcaac tggtcgcctt cattttgtaa
    13021 aatgttcgct tgcggccgaa aaatttcgat atatctacaa ttgatataca atctttacta
    13081 aattttgaaa aaggaacact ttgaatttcg aactgtcaat cgtatcatta gaatttaatc
    13141 taaatttaaa tcttgctaaa ggaaatagca aggaacactt tcgtcgtcgg ctacgcattc
    13201 attgtaaaat tttaaatttt gacattccgc actttttgat agataagcga agagtatttt
    13261 tattacatgt atcgcaagta ttcatttcaa cacacatatc tatatatata tgttatatat
    13321 ttattcaatt ttgtttacca ttgatcaatt tttcacacat gaaacaaccg ccagcattat
    13381 ataatttttt tattttttta aaaaatgtgt acacatattc tgaaaatgaa aaattcaatg
    13441 gctcgagtgc caaataaaga aatggttaca atttaaggaa acaaatgtcc ttcttgcgtt
    13501 tgaaacaact aatccttttc gccctcgcgg cgtttctcga aaagggccag gaaggatcca
    13561 ctagtgcgta tcgataagct ttaagataca ttgatgagtt tggacaaacc acaactagaa
    13621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    13681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
    13741 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    13801 attatgatct agagtcgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc
    13861 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
    13921 tcagggcgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
    13981 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
    14041 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
    14101 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
    14161 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
    14221 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
    14281 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca
    14341 ggccgtagcc gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
    14401 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
    14461 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
    14521 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcgtaccgt cgactctagc
    14581 ggtaccccga ttgtttagct tgttcagctg cgcttgttta tttgcttagc tttcgcttag
    14641 cgacgtgttc actttgcttg tttgaattga attgtcgctc cgtagacgaa gcgcctctat
    14701 ttatactccg gcggtcgagg gttcgaaatc gataagcttg gatcctaatt gaattagctc
    14761 taattgaatt agtctctaat tgaattagat ccccgggcga gctcgaatta acattgcaac
    14821 tttgtataca aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
    14881 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
    14941 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
    15001 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
    15061 cactggatat accaccgttg atatatcc
//
