LOCUS       sequence-406179-        2481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for phleomycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez 
            D, Marcos JF, Garrigues S
  TITLE     FungalBraid 2.0: expanding the synthetic biology toolbox for the 
            biotechnological exploitation of filamentous fungi.
  JOURNAL   Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 
            10.3389/fbioe.2023.1222812. eCollection 2023.
  PUBMED    37609115
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2023-08-7"; volume: "11:1222812. doi"; 
            pages: " 10.3389/fbioe.2023.1222812. eCollection 2023"
COMMENT     Sequence Label: FB413
FEATURES             Location/Qualifiers
     source          1..2481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..376
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      418..475
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(499..516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(670..1258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(750..769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1441..1535)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1556..2215)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2130..2149
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2377..2395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2399..2442)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2445..2466
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
       61 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
      121 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
      181 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
      241 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
      301 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
      361 cgaggagcag gactgagctt tgagaccact catcgcgact agtagcggcc gctgcagtcc
      421 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      481 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      661 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1081 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1441 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1501 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1561 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1621 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1681 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1741 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
     1801 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1861 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
     1921 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1981 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2041 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2101 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2161 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2221 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2281 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2341 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2401 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2461 ctagagcgat gagtggtctc g
//
