LOCUS       sequence-406181-       10998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For marking CRISPR HDR integrant with EGFP expressed in flight 
            muscles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT39
FEATURES             Location/Qualifiers
     source          1..10998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(273..932)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     847..866
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(986..992,993..1010,1011..1016))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(1039..1162)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2036..2749
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          2836..2901
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3031..3051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3323..3457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3360..3379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3414..3433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3526..3649
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3789..3808)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(4329..5120,5121..5189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4952..4971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5190..5294)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(join(5337..5343,5344..5361,5362..5367))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5390..5513)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5520..5940)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     promoter        complement(6017..6035)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6018..6035)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6067..6084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6238..6826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6318..6337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7072..7863)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     8316..8335
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8316..8334
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8384..9180
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     protein_bind    9233..9356
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(9395..10729,10730..10816))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(join(10817..10998,1..264))
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 cgcgcgattt acttttcgac ctcattctat tagactctcg tttggattgc aactggtcta
       61 ttttcctctt ttgtttgata gaaaatcata aaaggatttg cagactacgg gcctaaagaa
      121 ctaaaaaatc tatctgtttc ttttcattct ctgtattttt tatagtttct gttgcatggg
      181 cataaagttg cctttttaat cacaattcag aaaatatcat aatatctcat ttcactaaat
      241 aatagtgaac ggcaggtata tgtgccacgc gtttacgccc cgccctgcca ctcatcgcag
      301 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      361 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      421 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      481 acccagggat tggctgacac gaaaaacata ttctcaataa accctttagg gaaataggcc
      541 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      601 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      661 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
      721 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
      781 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
      841 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
      901 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
      961 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
     1021 ggggtgccta atgcggccca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     1081 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     1141 tcaacttttg tatacaaagt tgattatact cgaattcgtt gtcggctcat atacatgggc
     1201 gaaataattt cgaatatgtt ttaaaaataa ccaaagacat tagaaagaga tcgccaatac
     1261 ttatacatta tgtctatgtg tgccatgtgg tagcatgagc caaaaagctt ctcgaaatta
     1321 cgaattacat atagacgaat atgtatgtga ctttagtttc gaaataattt cgagaatttt
     1381 aaaaataacg cattcgttaa aagttcgcgt caattcgaat cgaattttcg attgtcgatt
     1441 tagtgtggat tgtcgaaaat cgttccgcct tcgaagttta ctgaaaggaa tcattgcgat
     1501 ctcgtgaatt gcttgtatga gaacacgcca ccatatcgag atacctgttc aaaatatcga
     1561 aatcgcgttc caaacggtag tagttaccag ttgagtgagt tgtggtgaca gtttaacctc
     1621 tctacatttg taataataag cattgttttg gcgtatcaat agtctgtaga ttttttcaca
     1681 aagaaacctg gagaaatgta cacaaatatc taaatgaaaa ttggcgttac gtaacaaata
     1741 ccaacgcccc gaaaatccgt atatatgcaa agcattctga tatatatata tataagtggt
     1801 atacatatac atattcgtat cggatccgtg atctatattt aggaaccctc gaccgccgga
     1861 gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac
     1921 acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatcgg
     1981 ggtaccgcta gagtcgacgg taccgcgggc cgcggctcga gggtaccaat caaacatgtc
     2041 caaaggtgaa gaactgttta ccggagtagt cccgatattg gttgaactcg acggcgatgt
     2101 caacggtcat aaattcagtg tgtccggcga gggtgagggc gacgccacat acggtaagct
     2161 gacgttgaag ttcatatgca ccacgggcaa gctgcccgtg ccatggccga cgttggtcac
     2221 gacgctgacg tatggtgtcc agtgtttcag ccgttacccc gatcatatga agcagcatga
     2281 ctttttcaag tcggcgatgc cggagggata cgttcaagag aggaccattt tcttcaagga
     2341 tgacggcaac tataagacgc gagcggaggt gaaatttgaa ggcgacacac tcgttaaccg
     2401 tattgagttg aagggcattg attttaagga ggatggcaac attctgggcc ataagttgga
     2461 atacaactac aactcgcata atgtgtatat aatggcagat aagcagaaaa acggaataaa
     2521 ggttaacttc aagattcgcc acaacataga ggacggttcc gtgcaacttg cagatcatta
     2581 ccaacagaac acacccattg gagatggccc agttctcttg ccagacaatc actacctttc
     2641 cacacagtcc gcgttgagca aggaccccaa tgaaaagcgg gaccacatgg tgttgctgga
     2701 gtttgtgacc gcagctggta ttacacacgg catggatgag ctctacaagt aatctagagg
     2761 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     2821 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     2881 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     2941 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     3001 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     3061 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     3121 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     3181 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     3241 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3301 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3421 gtccaaactc atcaatgtat cttatcatgt ctggatcgat ggcgcgcctc cggaacataa
     3481 tggtgcatct agatgcattc gcgaggtacc gagctcggag ctggacaact ttgtatagaa
     3541 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     3601 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgc gacgtgcaga
     3661 ctggctgtgt ataagggagc ctgacattat attccccaga acatcaggtt aatggcgttt
     3721 ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggaaaccggc
     3781 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg
     3841 taaagttcac gggagacttt atctgagagc aagcgcgcag acgcaagtgg aataaccatg
     3901 cgacggcccg gtgtatcgat aatatcactc tgtacatcca caaacagacg ataacggctc
     3961 tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa
     4021 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
     4081 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga
     4141 gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta
     4201 atacgctgct tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata
     4261 ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc
     4321 cggatccatt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4441 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
     4501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5281 ataggggttc cgcgtagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt
     5341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgcggccc atagtgactg
     5401 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     5461 attgatattt atatcatttt acgtttctcg ttcaacttta ttatacaaag ttgaggcccc
     5521 gacgttaaat tgaaaatagg tctatatata cgaactgagt ctggaaaaag aagttgagaa
     5581 ttataaaaag tagtgagcac tggcgccctc tctgcttggc gagctaacct tttcgcctct
     5641 tggctgagta ggtggcgttt cattctactc tgtaaaatta atgtagaatt gaaacactat
     5701 ggtcaaaaaa tacttagggg cataagttat aaaacgtatg aaatgaattt ttatcaacct
     5761 gggctattca aaaattttcg aattatttta tgtatttttt ttaatcgttt ttcattatag
     5821 gttaaaatac actttaaaag gaattctttc ctgtaaaata aatataaata aatatgcttt
     5881 attgacagaa aatttgatgt ttttgtattt gagtaggagc aatcacaggt gagcaaaaaa
     5941 cccatatgtt cggcttgtcg atcggatccc ctccgtacca agacttcgaa ttcggatccc
     6001 tcgagcgata cacacttcta tagtgtcacc taaatgcgtt taaaccttcc tgcaggtgac
     6061 gatttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6121 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6181 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6241 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6301 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6361 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6421 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6481 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6541 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6601 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6661 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6721 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6781 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6841 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6901 gcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc
     6961 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag
     7021 ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc
     7081 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     7141 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     7201 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     7261 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     7321 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     7381 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     7441 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     7501 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     7561 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     7621 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     7681 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     7741 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     7801 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc
     7861 catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
     7921 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     7981 ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     8041 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     8101 cttacgaacc gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca
     8161 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
     8221 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
     8281 ttacaacggc tctcccgctg acgcgcccgc gaaattaata cgactcacta tagggagact
     8341 cacatgcggc cgcctcgaga attctgacgt cttaattgaa ttcattttca acgtcctcga
     8401 tagtatagtg gttagtatcc ccgcctgtca cgcgggagaa cggggttcaa ttccccgtcg
     8461 gggagaatct gtgattcctt tttttttgct tttactctgt tatataaaca atttctgttt
     8521 taattgaatc taatttgcca ttgcttttag gaatctcagg catccagcaa gcgtttgtcc
     8581 gccgaatcgc ccatcagtga agaagatcct gtggcggcta cgaaaatctc cccggccatg
     8641 tcggcctcca cctccagcga aaaacccatc agcgagctgg ccacctctgt gctgacccac
     8701 cgctttccag actccacctc ctcacccggc gaacatggcc ttggacgaat gcagttgtcg
     8761 atccgctaca gcgcccagcg tcaaaaacta gacgtgacca tacacaaaat ccagaagata
     8821 ccacttcgcg atcccagcaa tatccccgat ccgtatgtta agctgtatct gttgcctgga
     8881 cgcaccaagg agtcgaaacg caagacgagc gtgatcaagg acaactgcaa ccccgtctac
     8941 gatgcatcct ttgagtacct gatttccatt gccgaactca ggcagacgga actggaggtg
     9001 acggtgtgca cccaaaaggg attcctatcc ggcggtagtc ccatcattgg catggtaggt
     9061 acccgaaagc aaccccttag ttacagacac agcgcgtacg tccttcgcat ccttatgatt
     9121 cccaagtaca tattctgcaa gagtacagta tatataggaa agatatccgg gtgaacttcg
     9181 attaagaagt ctacaggaat gggcatctag atgcaccatt atgttccgga ggcaactttg
     9241 tattaaaaag ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     9301 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatgcgac
     9361 gtgcagactg gctgtgtata agggagcctg acatttattt gttaactgtt aattgtcctt
     9421 gttcaaggat gctgtctttg acaacagatg ttttcttgcc tttgatgttc agcaggaagc
     9481 ttggcgcaaa cgttgattgt ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa
     9541 tcacgacatt gtttcctttc gcttgaggta cagcgaagtg tgagtaagta aaggttacat
     9601 cgttaggatc aagatccatt tttaacacaa ggccagtttt gttcagcggc ttgtatgggc
     9661 cagttaaaga attagaaaca taaccaagca tgtaaatatc gttagacgta atgccgtcaa
     9721 tcgtcatttt tgatccgcgg gagtcagtga acaggtacca tttgccgttc attttaaaga
     9781 cgttcgcgcg ttcaatttca tctgttactg tgttagatgc aatcagcggt ttcatcactt
     9841 ttttcagtgt gtaatcatcg tttagctcaa tcataccgag agcgccgttt gctaactcag
     9901 ccgtgcgttt tttatcgctt tgcagaagtt tttgactttc ttgacggaag aatgatgtgc
     9961 ttttgccata gtatgctttg ttaaataaag attcttcgcc ttggtagcca tcttcagttc
    10021 cagtgtttgc ttcaaatact aagtatttgt ggcctttatc ttctacgtag tgaggatctc
    10081 tcagcgtatg gttgtcgcct gagctgtagt tgccttcatc gatgaactgc tgtacatttt
    10141 gatacgtttt tccgtcaccg tcaaagattg atttataatc ctctacaccg ttgatgttca
    10201 aagagctgtc tgatgctgat acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat
    10261 gtttaccgga gaaatcagtg tagaataaac ggatttttcc gtcagatgta aatgtggctg
    10321 aacctgacca ttcttgtgtt tggtctttta ggatagaatc atttgcatcg aatttgtcgc
    10381 tgtctttaaa gacgcggcca gcgtttttcc agctgtcaat agaagtttcg ccgacttttt
    10441 gatagaacat gtaaatcgat gtgtcatccg catttttagg atctccggct aatgcaaaga
    10501 cgatgtggta gccgtgatag tttgcgacag tgccgtcagc gttttgtaat ggccagctgt
    10561 cccaaacgtc caggcctttt gcagaagaga tatttttaat tgtggacgaa tcgaattcag
    10621 gaacttgata tttttcattt ttttgctgtt cagggatttg cagcatatca tggcgtgtaa
    10681 tatgggaaat gccgtatgtt tccttatatg gcttttggtt cgtttctttc gcaaacgctt
    10741 gagttgcgcc tcctgccagc agtgcggtag taaaggttaa tactgttgct tgttttgcaa
    10801 actttttgat gttcatcgtt catgtctcct tttttatgta ctgtgttagc ggtctgcttc
    10861 ttccagccct cctgtttgaa gatggcaagt tagttacgca caataaaaaa agacctaaaa
    10921 tatgtaaggg gtgacgccaa agtatacact ttgcccttta cacattttag gtcttgcctg
    10981 ctttatcagt aacaaacc
//
