LOCUS       sequence-406184-       13856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked 
            with removable 3XP3-DsRed cassette expressed in eyes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 13856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT58
FEATURES             Location/Qualifiers
     source          1..13856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..456
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(457..543,544..1878)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(1917..2040)
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2132..2151)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2133..2151)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2604..3395
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3641..4229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4130..4149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4383..4400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4432..4450
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4432..4449
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        4527..4947
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     protein_bind    4954..5077
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5100..5105,5106..5123,5124..5130)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        5173..5277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5278..5346,5347..6138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5496..5515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6659..6678
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6818..6941)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    7080..7174
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    7208..7302
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    7336..7430
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    7464..7558
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        7583..7821
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7768..7789
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7836..7902
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7941..7958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(7998..8006,8007..8021)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             9282..9335
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(11482..11500)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     regulatory      11928..11937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    12722..12856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12759..12778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12813..12832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    12969..13092
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(13115..13120,13121..13138,13139..13145)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(13199..13856,1..2)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13265..13284)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaacgcgtgg cacatatacc tgccgttcac tattatttag tgaaatgaga tattatgata
       61 ttttctgaat tgtgattaaa aaggcaactt tatgcccatg caacagaaac tataaaaaat
      121 acagagaatg aaaagaaaca gatagatttt ttagttcttt aggcccgtag tctgcaaatc
      181 cttttatgat tttctatcaa acaaaagagg aaaatagacc agttgcaatc caaacgagag
      241 tctaatagaa tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa agcaggcaag
      301 acctaaaatg tgtaaagggc aaagtgtata ctttggcgtc accccttaca tattttaggt
      361 ctttttttat tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg aagaagcaga
      421 ccgctaacac agtacataaa aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa
      481 caagcaacag tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt
      541 gcgaaagaaa cgaaccaaaa gccatataag gaaacatacg gcatttccca tattacacgc
      601 catgatatgc tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc
      661 gattcgtcca caattaaaaa tatctcttct gcaaaaggcc tggacgtttg ggacagctgg
      721 ccattacaaa acgctgacgg cactgtcgca aactatcacg gctaccacat cgtctttgca
      781 ttagccggag atcctaaaaa tgcggatgac acatcgattt acatgttcta tcaaaaagtc
      841 ggcgaaactt ctattgacag ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa
      901 ttcgatgcaa atgattctat cctaaaagac caaacacaag aatggtcagg ttcagccaca
      961 tttacatctg acggaaaaat ccgtttattc tacactgatt tctccggtaa acattacggc
     1021 aaacaaacac tgacaactgc acaagttaac gtatcagcat cagacagctc tttgaacatc
     1081 aacggtgtag aggattataa atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta
     1141 cagcagttca tcgatgaagg caactacagc tcaggcgaca accatacgct gagagatcct
     1201 cactacgtag aagataaagg ccacaaatac ttagtatttg aagcaaacac tggaactgaa
     1261 gatggctacc aaggcgaaga atctttattt aacaaagcat actatggcaa aagcacatca
     1321 ttcttccgtc aagaaagtca aaaacttctg caaagcgata aaaaacgcac ggctgagtta
     1381 gcaaacggcg ctctcggtat gattgagcta aacgatgatt acacactgaa aaaagtgatg
     1441 aaaccgctga ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa
     1501 atgaacggca aatggtacct gttcactgac tcccgcggat caaaaatgac gattgacggc
     1561 attacgtcta acgatattta catgcttggt tatgtttcta attctttaac tggcccatac
     1621 aagccgctga acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc
     1681 tttacttact cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc
     1741 tatatgacaa acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc aagcttcctg
     1801 ctgaacatca aaggcaagaa aacatctgtt gtcaaagaca gcatccttga acaaggacaa
     1861 ttaacagtta acaaataaat gtcaggctcc cttatacaca gccagtctgc acgtcgcata
     1921 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     1981 ttaatatatt gatatttata tcattttacg tttctcgttc aactttttaa tacaaagttg
     2041 cctccggaac ataatggtgc atctagatgc ccattcctgt agacttctta attaagacgt
     2101 cagaattctc gaggcggccg catgtgagtc tccctatagt gagtcgtatt aatttcgcgg
     2161 gcgcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     2221 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     2281 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     2341 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2401 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2461 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2521 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2581 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     2641 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2701 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2761 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2821 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2881 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2941 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3001 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3061 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3121 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3181 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3241 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3301 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3361 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     3421 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     3481 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     3541 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3781 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4381 gcagcgagtc agtgagcgag gaaatcgtca cctgcaggaa ggtttaaacg catttaggtg
     4441 acactataga agtgtgtatc gctcgaggga tccgaattcg aagtcttggt acggagggga
     4501 tccgatcgac aagccgaaca tatgggtttt ttgctcacct gtgattgctc ctactcaaat
     4561 acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt ttacaggaaa
     4621 gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa aatacataaa
     4681 ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata cgttttataa
     4741 cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat tttacagagt
     4801 agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca agcagagagg
     4861 gcgccagtgc tcactacttt ttataattct caacttcttt ttccagactc agttcgtata
     4921 tatagaccta ttttcaattt aacgtcgggg cctcaacttt gtataataaa gttgaacgag
     4981 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     5041 cataatactg taaaacacaa catatccagt cactatgggc cgcattaggc accccaggct
     5101 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     5161 tttcaggagc tacgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5221 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5281 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5341 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5401 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5461 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5521 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5581 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5641 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5701 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5761 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5821 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5881 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5941 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc
     6001 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6061 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6121 ctgattaagc attggtaatg gatccggctt actaaaagcc agataacagt atgcgtattt
     6181 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     6241 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     6301 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     6361 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     6421 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     6481 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     6541 gatattatcg atacaccggg ccgtcgcatg gttattccac ttgcgtctgc gcgcttgctc
     6601 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     6661 atgatgacca ccgatatggc cagtgtgccg gtttccgtta tcggggaaga agtggctgat
     6721 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     6781 tgtcaggctc ccttatacac agccagtctg cacgtcgcat agtgactgga tatgttgtgt
     6841 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     6901 atcattttac gtttctcgtt caacttttct atacaaagtt gtccagctgg aggctcgcgt
     6961 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     7021 gacaggagca cgatcatgcg cacccgtggc cagggccgca agcttgcatg cctgcaggtc
     7081 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     7141 gtactgtcct ccgagcggag tactgtcctc cgagcggaga ctctagccct agggcatgcc
     7201 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     7261 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgctag
     7321 cgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     7381 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct
     7441 agcactagtg catgcctgca ggtcggagta ctgtcctccg agcggagtac tgtcctccga
     7501 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     7561 gagactctag cgacgtcgag cgccggagta taaatagagg cgcttcgtct acggagcgac
     7621 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     7681 gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa
     7741 agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt
     7801 gaatacaaga agagaactct gaatagatct aaaaggtagg ttcaaccact gatgcctagg
     7861 cacaccgaaa cgactaaccc taattcttat cctttacttc aggcggccgc aacttaaaaa
     7921 aaaaaatcaa aatgggctca catcatcacc accaccacgg aatggctagc atgacgggcg
     7981 gacaacagat gggccgcgat ctgtacgatg atgatgataa ggacctggcg acaatggttg
     8041 acagcagccg gcgcaagtgg aacaaaacag gacatgcagt cagagtcatt ggccgccttt
     8101 cctcactgga aaacgtctac ataaaggcgg ataagcagaa gaacggtatc aaagccaatt
     8161 tccatatccg ccacaacatc gaagatggcg gagtgcagct cgcataccac taccaacaga
     8221 atacgccaat cggagacggc cccgtgctgc tgcccgacaa ccattacctg agtgttcagt
     8281 cgaagcttag caaggaccca aacgaaaaac gagatcatat ggttctgctg gaatttgtga
     8341 ctgctgctgg tattaccctg ggcatggatg aactctataa gggtggaacg ggaggctcca
     8401 tggttagcaa aggcgaggag ctgttcactg gcgtcgtgcc gatcctggtc gagttggacg
     8461 gagatgttaa tggacacaaa ttcagtgtga gtggcgaggg cgagggcgat gcgacatacg
     8521 gcaagttgac cttgaagttc atttgcacca ccggtaaact gccagtaccc tggcccacgc
     8581 tggtcaccac gctcacctat ggcgtccagt gcttctctcg ttatccggat cacatgaagc
     8641 agcatgattt cttcaagtcc gccatgcctg aggggtacat tcaagagcga acaatcttct
     8701 tcaaggacga cggcaattac aagaccagag ccgaagtgaa gtttgaaggc gatacactag
     8761 tgaaccgcat cgagctaaag ggtattgatt ttaaggagga tggcaacatc ttgggccaca
     8821 agctggagta caaccttccc gatcagctaa ccgaggagca gatcgcggag ttcaaggaac
     8881 tgttttcgct gttcgacaaa gatggtgatg gtacgattac cacgaaggaa ttgggaacag
     8941 tgatgcggtc cctggggcag aaccccaccg aggccgagct gcaggatatg ataaacgagg
     9001 tggatgctga tggtgacggc acgattgatt ttcccgagtt cttgaccatg atggcccgaa
     9061 agatgaagta ccgcgataca gaggaggaga ttagggaggc attcggtgtg ttcgacaagg
     9121 acggcaatgg atacattagt gcagcagagt tgcgccacgt tatgactaac ctgggtgaga
     9181 agttgactga cgaagaggtc gatgagatga ttagggaagc cgatattgac ggcgatggcc
     9241 aggtgaacta tgaggaattc gtacaaatga tgaccgccaa agagggtcgc ggatcgctgc
     9301 tgacctgcgg cgacgtggag gaaaaccccg gccccgttag caagggtgaa gaggtgatta
     9361 aagaattcat gcgttttaaa gtacgcatgg agggtagcat gaacggtcat gagtttgaga
     9421 tcgagggcga aggcgaaggt cgtccgtacg agggtacgca gaccgctaaa ctgaaagtaa
     9481 ccaaaggcgg tcctctgccg ttcgcgtggg acatcctgtc cccgcagttt atgtatggtt
     9541 ccaaggccta cgtcaaacac ccagccgata tcccggacta caagaagctg tctttcccgg
     9601 agggtttcaa gtgggagcgt gtaatgaact ttgaggacgg tggcctggtt accgtcaccc
     9661 aggatagctc tctgcaagat ggcaccctga tctacaaggt gaagatgcgt ggtacgaact
     9721 ttccgccgga tggcccggtg atgcagaaaa agacgatggg atgggaagca tctaccgagc
     9781 gcctgtatcc gcgtgacggt gttctgaaag gtgagatcca ccaggcgctg aaactgaagg
     9841 acggcggtca ctacctggtt gaatttaaga cgatctatat ggctaaaaag ccggttcaac
     9901 tgccaggtta ctactacgtg gacaccaaac tggatatcac gtctcacaac gaagattata
     9961 ccatcgtcga acagtatgaa cgtagcgagg gccgtcacca cctgttcctg ggccacggta
    10021 ctggttctac gggttctggt tcctctggca ctgcgtcttc cgaagacaac aacatggcgg
    10081 tgatcaaaga gttcatgagg ttcaaagtac gtatggaagg ttctatgaac ggccatgagt
    10141 tcgagattga aggtgagggc gagggtcgcc catacgaggg cactcagacc gcaaagctga
    10201 aagttaccaa aggtggccca ctgccttttg cgtgggatat cctgtctccg cagttcatgt
    10261 acggttccaa agcctacgta aaacacccgg cagacatccc ggattacaaa aagctgtcct
    10321 tcccagaggg cttcaaatgg gaacgtgtta tgaatttcga agatggcggc ctggtcactg
    10381 ttacgcagga cagcagcctg caagacggta ctctgattta caaagtgaaa atgcgtggca
    10441 ctaacttccc gcctgacggt ccagtaatgc agaagaaaac tatgggctgg gaagctagca
    10501 ccgaacgtct gtacccgcgc gacggtgtgc tgaaaggcga gattcaccag gccctgaagc
    10561 tgaaggatgg tggtcactat ctggttgagt tcaaaactat ctacatggca aaaaagcctg
    10621 tacaactgcc gggctattac tacgttgaca ctaaactgga catcacctcc cacaacgagg
    10681 attatactat tgttgaacag tacgagcgtt ctgaaggtcg ccatcatctg tttctgtatg
    10741 gtatggacga actgtataaa taaggtgtac aaatctagaa tgaatcgttt ttaaaataac
    10801 aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt
    10861 aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac
    10921 gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct
    10981 ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg
    11041 tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt
    11101 tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca
    11161 taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc
    11221 gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca
    11281 aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg
    11341 ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc
    11401 acgatgccga caatggagct ggcttggata gcgattcgag ttaacggccg gccgtttaaa
    11461 cgaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    11521 taataatggt ttcttagact caggtggcac ttttcgggga aaggttgctt aagaataagt
    11581 aattcttaag caaccattac gccaagcttg catgcaggcc tctgcagtcg acgggcccgg
    11641 gatccgatcg acaagccgaa catatgggcg cgccgggatc taattcaatt agagactaat
    11701 tcaattagag ctaattcaat taggatccaa gcttatcgat ttcgaaccct cgaccgccgg
    11761 agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa
    11821 cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatcg
    11881 gggtaccgct agagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtgc
    11941 gctcctccaa gaacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggcaccg
    12001 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca
    12061 acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
    12121 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
    12181 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaat ttcgaagatg
    12241 gtggagttgt cacagtcaca caagattctt cgctccaaga tggttgtttt atttacaaag
    12301 tgaaatttat tggagtcaat tttccgtcgg atggaccggt tatgcaaaag aaaacaatgg
    12361 gttgggaagc atcgacggaa cgtctgtatc cacgagatgg agttttgaaa ggcgaaattc
    12421 acaaagctct taaactcaaa gatggcggac attaccttgt agaatttaaa agcatttata
    12481 tggctaagaa accggtccaa ctccctggat attattatgt cgattcgaaa ctggatatta
    12541 cttcacataa cgaagattat acgatagttg agcaatatga acgcactgaa ggtcgtcacc
    12601 atctctttct ctaaagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    12661 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    12721 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    12781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    12841 ttatcatgtc tggatcgatg gcgcgcctcc ggaacataat ggtgcatcta gatgcattcg
    12901 cgaggtaccg agctcgaatt cactggggtt gcttaagaat aagtaattct taagcaaccc
    12961 aaccccatca actttgtata caaaagttga acgagaaacg taaaatgata taaatatcaa
    13021 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
    13081 ccagtcacta tgggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    13141 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
    13201 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
    13261 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
    13321 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
    13381 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
    13441 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
    13501 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
    13561 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
    13621 tatgtttttc gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
    13681 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
    13741 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
    13801 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgt
//
