LOCUS       sequence-406188-       13161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For introducing heat shock inducble B3 recombinase. HDR integrant 
            marked with ie1-EGFP, expressed in abdomen and mouthparts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Stern  D, Kim E, Behrman E
  TITLE     The Janelia Atalanta plasmids provide a simple and efficient 
            CRISPR/Cas9-mediated homology directed repair platform for 
            Drosophila
  JOURNAL   bioRxiv 2023.06.17.545412
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.06.17.545412"
COMMENT     Sequence Label: pJAT43
FEATURES             Location/Qualifiers
     source          1..13161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1845..2301)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1877..1898)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    2348..2469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2592..3305,3306..3308,3309..3311))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2595..3305,3306..3308,3309..3311))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2637..2658)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2986..3005
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3245..3266
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3308..3317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3502..4093)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        4097..4579
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     protein_bind    4608..4731
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4871..4890)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(5411..6202,6203..6271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6034..6053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6272..6376)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(join(6419..6425,6426..6443,6444..6449))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6472..6595)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6602..7022)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     promoter        complement(7099..7117)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7100..7117)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7149..7166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7320..7908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7400..7419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8154..8945)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     9398..9417
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9398..9416
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9509..9632
                     /gene="mutant version of attR"
                     /label=attR6
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(9671..11005,11006..11092))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(11093..11538)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             complement(11547..12206)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     12121..12140
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(12260..12266,12267..12284,12285..12290))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(12313..12436)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     12438..12456
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    12498..12632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(12904..12924)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          13054..13119
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cacaccacag aagtaaggtt ccttcacaaa gatcctctag aggtaccctt accggttaat
       61 tggtgccagg acgcgccgcc gcttcgactc aacggtgcgg cgaccaccca cgccacgttt
      121 acggccggca ccggcggcac ggccagtctg actcggaggg gaatagacca cgttggagtt
      181 ggctttcgac atgtccaggt aaattccgct aatgttactt gccgttgttt cgtcctcgga
      241 gtcggacatt tcgataggta tctcttcgct ttgcgacgaa tccgaatcta tcttctcaat
      301 ctcattatca tcgttggcat gttcgctggc acgcaacttt cggccctcgc ttggggaggc
      361 ttttcccata ctaagtattg gaatcctgac gggactgctc ggctcttcgc ttcccttgga
      421 gccgctcaag gtgacggact ctgtcttaag gaacgtacgt gcgtacaatg tggagttctg
      481 atatcgccgg catgcgttct tcgaatgata tcgcttcaga aatatcagaa catccttgct
      541 cacaattttg gcccactcgc cgtagctttc acaaatctct gtatcctcct tctcattaag
      601 tgggtccata ataccgtcaa acagatcatt aatgtccttc tccagaccca agcccacgat
      661 gtggccctct tcatttcgaa cgcagtagcc agccaggaat ccgaacagtg gctgtggcaa
      721 atctcgacca ccgtgggtgt agcccagtcg cgcaccggac tcgcgttgat tctgggagga
      781 gctccagttg cccagggagt tggcccagga atccagttca ttcttgtgca gaaagctgtt
      841 catcagatgc cggcccagat gggacttggg gccgtgcttt attgcgaaaa taggcgaagc
      901 tggaaacttc ctaaagaaac ggttgtaatc aatgaccatg gaatctcgga aaagctgcca
      961 cttctgctcc gatacttgat ccgaaagacg cgactttggg gtaggctggc atgtgcggaa
     1021 gaaatcgtgc aaggccagga acagatcgaa tcgattctcg cggatgggaa agaagaaaat
     1081 gttcctcgac ttgcgtgtct tagtctgttt gacctcagcc tgcagcaggt atccgacgaa
     1141 tttgtttttc acaatcttaa atgtggaggg atcaacgttc atcaggtcat tgttcctaca
     1201 gcagttgaaa aatgtcatca acaggatgaa agtcagcacc gtcttttggc gatccgtgtt
     1261 cgtttgtgtc tcaatactat ccaacgtgtt caagatgaat ttgaaaatcg attcgtctgt
     1321 ctcggtgcta tgaaacaagt tatccacctc gcggtccacc tctttcttcc gccagttctt
     1381 ctggctgcca aacgaggggc cacgaaagag agtcttaaac gaggttgcct ctttattcga
     1441 cttaattttg aatttgtagg gctcgaaggc atcgctcaag cccctggaca gctcatcaca
     1501 gtccttcaag tgatacacaa acgagatagt cgacgaaccc acgtcatact ggaggccctg
     1561 cttgattgtt gtcttatatt tgttaaaggt tgtcttttta acttcggtca gcgccttgat
     1621 tgccttaagg atcgccatgg taataattcc actcagcttg tctccgcaga agttctcgcc
     1681 cgctttcaag cactccacga acttatggta caaagtcgct ggttcatcat caacaagatc
     1741 catatacgag ctcattttga ttctcgagcc gcggccgcct gaagtaaagg ataagaatta
     1801 gggttagtcg tttcggtgtg cctagccgcg gccgcagatc tattcagagt tctcttcttg
     1861 tattcaataa ttacttcttg gcagatttca gtagttgcag ttgatttact tggttgctgg
     1921 ttacttttaa ttgattcact ttaacttgca ctttactgca gattgtttag cttgttcagc
     1981 tgcgcttgtt tatttgctta gctttcgctt agcgacgtgt tcactttgct tgtttgaatt
     2041 gaattgtcgc tccgtagacg aagcgcctct atttatactc cggcgctcga cgtttttcgc
     2101 gaacattcga ggcgcgctct ctcgaaccaa cgagagcagt atgccgttca ctgtgtgaca
     2161 gagtgagaga gcattagtgc agagagggag agacccaaaa agaaaagaga gaataacgaa
     2221 taacggccag agaaatttct cgatttttct ttctgccaaa caaatgacct accacaataa
     2281 ccagtttgtt ttgggattct aacgtcgtgt tcggcttgta agtctggaaa cgcgtatcga
     2341 taagctttaa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2401 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2461 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2521 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
     2581 tcgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     2641 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     2701 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     2761 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     2821 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     2881 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     2941 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     3001 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     3061 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     3121 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     3181 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     3241 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     3301 ttgctcacca tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga ctctagcggt
     3361 accccgattg tttagcttgt tcagctgcgc ttgtttattt gcttagcttt cgcttagcga
     3421 cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg cctctattta
     3481 tactccggcg gtcgagggtc acttggttgt tcacgatctt gtcgccgcca gtgtcaactt
     3541 gcaactgaaa caatatccaa catgaacgtc aatttatact gccctaatgg cgaacacgat
     3601 aacaatattt cttttattat gccctctaaa accaacgcgg ttatcgttta tttattcaaa
     3661 ttagatatag aacatccgcc gacatacaat gttaatgcaa aaacgcgttt ggtgagcgga
     3721 tacgaaaaca gtcggccgat aaacattaat ctgaggtcga taacaccgtc cttgaacgga
     3781 acacgaggag cgtacgtgat cagctgcatt cgcgcgccgc gcctttatcg agatttattt
     3841 gcatacaaca agtacactgc gccgttggga tttgtggtaa cgcgcacaca tgcagagctg
     3901 caagtgtggc acattttgtc tgtgcgcaaa acctttgaag ccaaaagtac gaggtccgtt
     3961 acgggcatgc tagcgcacac ggacaatgga cccgacaaat tctacgccaa ggatttaatg
     4021 ataatgtcgg gcaacgtatc cgttcatttt atcaataacc tacaaaaatg tcgcgcgcat
     4081 cacaaagaca tcgacgcgcg tagaattcta cccgtaaagc gagtttagtt atgagccatg
     4141 tgcaaaacat gacatcagct tttattttta taacaaatga catcatttct tgattgtgtt
     4201 ttacacgtag aattctactc gtaaagcgag ttcagttttg aaaaacaaat gacatcatct
     4261 ttttgattgt gctttacaag tagaattcta cccgtaaatc aagttcggtt ttgaaaaaca
     4321 aatgagtcat attgtatgat atcatattgc aaaacaaatg actcatcaat cgatcgtgcg
     4381 ttacacgtag aattctactc gtaaagcgag tttatgagcc gtgtgcaaaa catgacatca
     4441 tctcgatttg aaaaacaaat gacatcatcc actgatcgtg cgttacaagt agaattctac
     4501 tcgtaaagcc agttcggtta tgagccgtgt gcaaaacatg acatcagctt atgactcata
     4561 cttgattgtg ttttacgcgg gcgcgtaaga gctcggtgca agctggacaa ctttgtatag
     4621 aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     4681 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat gcgacgtgca
     4741 gactggctgt gtataaggga gcctgacatt atattcccca gaacatcagg ttaatggcgt
     4801 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggaaaccg
     4861 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     4921 ggtaaagttc acgggagact ttatctgaga gcaagcgcgc agacgcaagt ggaataacca
     4981 tgcgacggcc cggtgtatcg ataatatcac tctgtacatc cacaaacaga cgataacggc
     5041 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
     5101 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     5161 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
     5221 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     5281 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     5341 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     5401 gccggatcca ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5521 catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat
     5581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6361 aaataggggt tccgcgtagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca
     6421 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc ccatagtgac
     6481 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     6541 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgaggcc
     6601 ccgacgttaa attgaaaata ggtctatata tacgaactga gtctggaaaa agaagttgag
     6661 aattataaaa agtagtgagc actggcgccc tctctgcttg gcgagctaac cttttcgcct
     6721 cttggctgag taggtggcgt ttcattctac tctgtaaaat taatgtagaa ttgaaacact
     6781 atggtcaaaa aatacttagg ggcataagtt ataaaacgta tgaaatgaat ttttatcaac
     6841 ctgggctatt caaaaatttt cgaattattt tatgtatttt ttttaatcgt ttttcattat
     6901 aggttaaaat acactttaaa aggaattctt tcctgtaaaa taaatataaa taaatatgct
     6961 ttattgacag aaaatttgat gtttttgtat ttgagtagga gcaatcacag gtgagcaaaa
     7021 aacccatatg ttcggcttgt cgatcggatc ccctccgtac caagacttcg aattcggatc
     7081 cctcgagcga tacacacttc tatagtgtca cctaaatgcg tttaaacctt cctgcaggtg
     7141 acgatttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7741 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7981 atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     8041 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg
     8101 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg
     8161 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     8221 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     8281 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     8341 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     8401 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     8461 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     8521 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     8581 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     8641 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     8701 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     8761 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     8821 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     8881 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     8941 cccatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     9001 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     9061 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     9121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     9181 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg cccgaattga tcacaggcag
     9241 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     9301 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     9361 ccttacaacg gctctcccgc tgacgcgccc gcgaaattaa tacgactcac tatagggaga
     9421 ctcacatgcg gccgcctcga gaattctgac gtcttaatta agaagtctac aggaatgggc
     9481 atctagatgc accattatgt tccggaggca actttgtatt aaaaagttga acgagaaacg
     9541 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     9601 tactgtaaaa cacaacatat ccagtcacta tgcgacgtgc agactggctg tgtataaggg
     9661 agcctgacat ttatttgtta actgttaatt gtccttgttc aaggatgctg tctttgacaa
     9721 cagatgtttt cttgcctttg atgttcagca ggaagcttgg cgcaaacgtt gattgtttgt
     9781 ctgcgtagaa tcctctgttt gtcatatagc ttgtaatcac gacattgttt cctttcgctt
     9841 gaggtacagc gaagtgtgag taagtaaagg ttacatcgtt aggatcaaga tccattttta
     9901 acacaaggcc agttttgttc agcggcttgt atgggccagt taaagaatta gaaacataac
     9961 caagcatgta aatatcgtta gacgtaatgc cgtcaatcgt catttttgat ccgcgggagt
    10021 cagtgaacag gtaccatttg ccgttcattt taaagacgtt cgcgcgttca atttcatctg
    10081 ttactgtgtt agatgcaatc agcggtttca tcactttttt cagtgtgtaa tcatcgttta
    10141 gctcaatcat accgagagcg ccgtttgcta actcagccgt gcgtttttta tcgctttgca
    10201 gaagtttttg actttcttga cggaagaatg atgtgctttt gccatagtat gctttgttaa
    10261 ataaagattc ttcgccttgg tagccatctt cagttccagt gtttgcttca aatactaagt
    10321 atttgtggcc tttatcttct acgtagtgag gatctctcag cgtatggttg tcgcctgagc
    10381 tgtagttgcc ttcatcgatg aactgctgta cattttgata cgtttttccg tcaccgtcaa
    10441 agattgattt ataatcctct acaccgttga tgttcaaaga gctgtctgat gctgatacgt
    10501 taacttgtgc agttgtcagt gtttgtttgc cgtaatgttt accggagaaa tcagtgtaga
    10561 ataaacggat ttttccgtca gatgtaaatg tggctgaacc tgaccattct tgtgtttggt
    10621 cttttaggat agaatcattt gcatcgaatt tgtcgctgtc tttaaagacg cggccagcgt
    10681 ttttccagct gtcaatagaa gtttcgccga ctttttgata gaacatgtaa atcgatgtgt
    10741 catccgcatt tttaggatct ccggctaatg caaagacgat gtggtagccg tgatagtttg
    10801 cgacagtgcc gtcagcgttt tgtaatggcc agctgtccca aacgtccagg ccttttgcag
    10861 aagagatatt tttaattgtg gacgaatcga attcaggaac ttgatatttt tcattttttt
    10921 gctgttcagg gatttgcagc atatcatggc gtgtaatatg ggaaatgccg tatgtttcct
    10981 tatatggctt ttggttcgtt tctttcgcaa acgcttgagt tgcgcctcct gccagcagtg
    11041 cggtagtaaa ggttaatact gttgcttgtt ttgcaaactt tttgatgttc atcgttcatg
    11101 tctccttttt tatgtactgt gttagcggtc tgcttcttcc agccctcctg tttgaagatg
    11161 gcaagttagt tacgcacaat aaaaaaagac ctaaaatatg taaggggtga cgccaaagta
    11221 tacactttgc cctttacaca ttttaggtct tgcctgcttt atcagtaaca aacccgcgcg
    11281 atttactttt cgacctcatt ctattagact ctcgtttgga ttgcaactgg tctattttcc
    11341 tcttttgttt gatagaaaat cataaaagga tttgcagact acgggcctaa agaactaaaa
    11401 aatctatctg tttcttttca ttctctgtat tttttatagt ttctgttgca tgggcataaa
    11461 gttgcctttt taatcacaat tcagaaaata tcataatatc tcatttcact aaataatagt
    11521 gaacggcagg tatatgtgcc acgcgtttac gccccgccct gccactcatc gcagtactgt
    11581 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
    11641 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
    11701 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
    11761 ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
    11821 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
    11881 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
    11941 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
    12001 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
    12061 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
    12121 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
    12181 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
    12241 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
    12301 cctaatgcgg cccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
    12361 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact
    12421 tttgtataca aagttgaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcgttt
    12481 aaacggccgg ccagatcgat ccagacatga taagatacat tgatgagttt ggacaaacca
    12541 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
    12601 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
    12661 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
    12721 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt ccataatttt
    12781 cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
    12841 attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
    12901 ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
    12961 atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
    13021 ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
    13081 tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
    13141 aggtagtttg tccaattatg t
//
