LOCUS       sequence-406210-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of fluorescent N-terminally tagged shRNA 
            resistant Sec22b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Criado Santos N, Bouvet S, Cruz Cobo M, Mandavit M, Bermont F, 
            Castelbou C, Mansour F, Azam M, Giordano F, Nunes-Hasler P
  TITLE     Sec22b regulates phagosome maturation by promoting ORP8-mediated 
            lipid exchange at endoplasmic reticulum-phagosome contact sites.
  JOURNAL   Commun Biol. 2023 Oct 4;6(1):1008. doi: 10.1038/s42003-023-05382-0.
  PUBMED    37794132
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-10-4"; volume: "6(1):1008. doi"; pages: " 
            10.1038/s42003-023-05382-0"
COMMENT     Sequence Label: pEGFP-Sec22b-shR
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    833..954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(870..889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     924..943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtccggactc agatctcgag ctcaagcttc gaattccgga tccgtgctgc tgacaatgat
       61 tgcccgcgta gcggacgggc ttcctctggc cgcctcgatg caggaggacg agcagtcagg
      121 ccgggacctt cagcagtatc agagtcaggc taagcaactc tttcgaaagt tgaatgaaca
      181 gtcccctacc agatgtacct tggaagcagg agccatgact tttcactaca ttattgagca
      241 gggggtgtgt tacttggttt tgtgtgaagc tgccttccct aagaagttgg cctttgccta
      301 cctagaagac ttgcactcgg aatttgacga gcagcatggg aagaaggtgc ccactgtgtc
      361 taggccttat tcttttatcg agttcgacac cttcattcag aagaccaaga aactctacat
      421 tgatagccgt gctcggagaa atctcggctc catcaacacc gaattgcaag atgtgcagag
      481 gatcatggtg gcgaatattg aagaagtcct acagcgggga gaagcactct cagcattgga
      541 ttcgaaggct aacaatttgt ccagtctatc taagaaatac cgccaggatg cgaagtacct
      601 gaacatgcgt tccacctacg ccaagcttgc agcagtagcc gtgttcttca tcatgttgat
      661 agtgtatgtg cggttttggt ggctgtgagg taccgcgggc ccgggatcca ccggatctag
      721 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      781 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
      841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
      961 aaattgtaag cgt
//
