LOCUS       sequence-406217-        6770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 
            (luminopsin LMO7) for bioluminescent optogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Bjorefeldt A, Murphy J, Crespo EL, Lambert GG, Prakash M, Ikefuama 
            EC, Friedman N, Brown TM, Lipscombe D, Moore CI, Hochgeschwender U, 
            Shaner NC
  TITLE     Efficient opto- and chemogenetic control in a single molecule driven
            by FRET-modified bioluminescence.
  JOURNAL   Neurophotonics. 2024 Apr;11(2):021005. doi: 
            10.1117/1.NPh.11.2.021005. Epub 2024 Mar 6.
  PUBMED    38450294
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neurophotonics."; date: "2024-04"; volume: "11(2):021005. doi"; 
            pages: " 10.1117/1.NPh.11.2.021005. Epub 2024 Mar 6"
COMMENT     Sequence Label: pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)
FEATURES             Location/Qualifiers
     source          1..6770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..134
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        179..626
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      943..952
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1909..1918
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2853..2870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2901..3489
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2954..2974)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3372..3383)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3521..3997
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3720..3739)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4037..4177
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4037..4166
                     /label=AAV2 ITR
     rep_origin      4252..4707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4339..4358)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4549..4570
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4724..4743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4843..4865
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4903..4921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4989..5093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5094..5162,5163..5954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5312..5331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6125..6713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6614..6633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac
       61 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
      121 cactaggggt tcctgcggcc gcacgcgtgt gtctagactg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagtcga gaaggtacca tgggagtcaa agttctgttt
      661 gccctgatct gcatcgctgt ggccgaggcc gtgtctaaag gagaggagga taatatggct
      721 agtctgcccg ctactcacga gctgcacatt tttgggagca tcaacggggt ggattttgac
      781 atggtgggcc agggcacagg caaccctaat gatggctacg aggagctgaa tctgaagtcc
      841 accaagggcg acctgcagtt ttctccttgg atcctggtgc cacacatcgg ctatggcttc
      901 caccagtatc tgccctaccc tgacggcatg tctccatttc aggccgccat ggtggatggc
      961 agcggctacc aggtgcaccg gaccatgcag ttcgaggacg gcgccagcct gacagtgaac
     1021 tacagatata cctacgaggg ctcccacatc aagggcgagg cccaggtgaa gggcacaggc
     1081 tttccagccg atggccccgt gatgacaaac agcctgaccg ccgccgactg gtgcagatcc
     1141 aagaagacct accccaatga taagacaatc atctctacct tcaagtggag ctataccaca
     1201 ggcaacggca agcggtacag atctacagcc aggaccacat atacctttgc caagccaatg
     1261 gccgccaact atctgaagaa tcagcccatg tacgtgttcc gcaagacaga gctgaagcac
     1321 agcaagaccg agctgaactt caaggagtgg cagaaggcct tcacaggctt tgaggacttc
     1381 gtgggcgatt gggagcagac cgccgcctat aacctggatc aggtgctgga gcagggaggc
     1441 gtgagctccg tgctgcagac actggccgtg tccgtgaccc ctatccagag gatcgtgcgc
     1501 tctggcgaga atggcctgaa gatcgacatc cacgtgatca tcccatacga gggcctgtcc
     1561 gccgatcaga tggcccacat cgaggaggtg tttaaggtgg tgtaccccgt ggacgatcac
     1621 cacttcaaag tgatcatgga gtatggcacc ctggtcatcg acggagtgac cccaaacatg
     1681 ctgaattatt ttggccggcc ttacgagggc atcgccgtgt tcgatggcaa gaagatcacc
     1741 gtgacaggca ccctgtggaa cggcaataag atcatcgacg agcggctgat cacccctgat
     1801 ggctctatgc tgttcagagt gaccatcaac ggcgtgagcg gctggcggct gttcgagaag
     1861 atttcaaacg gcgacccact agtccagtgt ggtggaattg ccggatccgc caccatggat
     1921 taccctgttg ctagaagcct catagttcgc tacccaaccg acctcggaaa cggcaccgtc
     1981 tgcatgccaa gaggacagtg ttactgtgaa ggttggcttc ggagtcgcgg cacttccatt
     2041 gaaaagacaa tagcaattac tcttcagtgg gtagtctttg ctttgtcagt ggcttgcctg
     2101 gggtggtatg cgtatcaagc gtggcgagct acctgcggat gggaggaggt ttacgtagcc
     2161 ttgatagaaa tgatgaaaag catcatcgag gccttccacg agttcgacag ccctgcaaca
     2221 ctgtggctgt cttcagggaa cggcgtagtt tggatgcggt atggcgaatg gctcctcacc
     2281 tgcccggtcc ttctgatcca tctgagcaac ctcacaggcc tgaaggacga ttatagcaaa
     2341 aggactatgg gcctgttggt ttctgatgtg ggatgcatcg tgtggggcgc aaccagcgcc
     2401 atgtgtacgg ggtggacgaa gatcctgttc ttcctcatct cattgagcta tggtatgtat
     2461 acctattttc atgctgctaa agtttatatc gaagcattcc acacagttcc aaaagggatt
     2521 tgtcgagaac tggtccgagt gatggcctgg acattctttg tggcttgggg aatgtttcca
     2581 gtcctgtttc tgctgggcac ggaaggattc ggtcatatca gcccttatgg atctgccatt
     2641 gggcactcca tcctcgacct gattgcaaag aacatgtggg gtgtgctggg gaattacctg
     2701 cgcgtcaaaa tccacgagca catcctgttg tatggcgaca tcagaaagaa gcagaaaatt
     2761 acgatcgccg gccaagagat ggaggttgag acactggtgg ctgaagagga ggacgcggcc
     2821 gcctaacata tggaattccc acgcgtaccg gtcatcatca ccatcaccat tgagtttaat
     2881 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2941 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3001 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3061 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3121 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3181 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3241 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3301 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     3361 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3421 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3481 cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
     3541 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     3601 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     3661 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     3721 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     3781 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     3841 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     3901 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     3961 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     4021 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4081 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4141 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     4201 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     4261 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4321 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4381 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4441 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4501 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4561 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4621 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4681 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4741 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4801 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4861 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4921 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5101 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5161 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5221 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5281 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5341 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5401 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5461 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5521 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5581 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5641 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5701 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5761 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5821 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5881 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5941 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
//
