LOCUS       sequence-406237-        6363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Duvenhage virus G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_DUVV_G
FEATURES             Location/Qualifiers
     source          1..6363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3068..3087)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3133..3188
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3187..3206)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3546..3562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3546..3562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3559..3581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3570..3586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3594..3600,3601..3618,3619..3624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3639..3660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3777..3794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3948..4536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4028..4047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4707..5498,5499..5567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5330..5349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5568..5672)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5698..6153)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5835..5856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6047..6066
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6280..6302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6294..6311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6295..6311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6321..6340
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6321..6339
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgcct ctgaatgccg
     1441 tgatctttac actgctgctg agatgctcca tctgtctggg caagttcccc atctacacca
     1501 tccccgataa actgggccct tggagcccca tcgacattca ccatctgagc tgccccaata
     1561 atctcgtggt cgaggatgaa ggctgtacca ccctcacacc cttttcctac atggagctca
     1621 aggtgggcta catcacctcc attaaggtgt ccggcttcac atgtaccggc gtggtgaccg
     1681 aggccgaaac ctacaccaac tttgtcggct acgtgaccac aacctttaga aggaggcact
     1741 tcagacctag cgtcaacagc tgtagggacg cctacaattg gaagatcgcc ggcgatccta
     1801 gatatgaaga atctctgcac aatccctacc ccgacagcca ctggctgaga accgtcaaaa
     1861 ccaccaagga gtctctgctg atcatcagcc ccagcgtggc cgatatggac gcctatgata
     1921 agaagctgta ctccaagatg ttccccaacg gaaggtgcag cgaaattagc cccggctccc
     1981 ccttctgtcc caccaaccac gagtacacaa tctggatgcc cgagagcagc aaccccggca
     2041 ttagctgcga catcttcaca agatccatgg gcaagaaggc caccaaagac ggccagctgt
     2101 gtggattcgt ggacgagaga ggactgtata aatctctgaa gggcgcttgt agactgagac
     2161 tctgcggcat ctccggactg agactgatgg atggaagctg ggtgtccctc ccccaagtga
     2221 acaacagcga atggtgctcc cccgaccaac tcgtcaacat ccatgacttc cattccgacg
     2281 agatcgagca tctcgtggcc gacgagctgg tgaagaagag agaggactgt ctggatgccc
     2341 tcgagaccat catcttcacc aagagcatca gctttagaag gctgagcaga ctgagaaaac
     2401 tggtccccgg cttcggcaag gcttatacca tcattaatag gaccctcatg gaggccgagg
     2461 cccattacaa gagcgtgaga gagtggaagg agatcatccc ctccaagggc tgtctcaagg
     2521 ccggcggaag gtgctacccc caccataacg gcatcttctt caacggcatc atcctcggac
     2581 ccggaggcga gatcctcatc cccgagatgc agtccgctct gctgcagcag cacatcgaac
     2641 tgctggaaag ctccgtcgtg cctctgaagc atcccctcgc cgaccctagc accgtgttca
     2701 agaatgacga cgaggccgag tcctttgtgg atgtccacct ccccgacacc aaccaaaaaa
     2761 tcagcggcat tgacctcgga ctgcccgagt ggaagaggta ctttctcatc ggagtgagcg
     2821 ccgtcgctct gctcgctctg tccatcatca ttgctgtctg ctgcaagagg tttagaaaga
     2881 gaagaaagag caagccttcc cccgtggagc tgacaagaaa ggtctccgtg atctccaagg
     2941 gcaatggccc cgtgcccagc tgggagagct ataaagaggg caccaccgga gatgtgagaa
     3001 acacaacccc ttccacaaga gagtgatgtc ttcctggagc tccagtcatt gactataacg
     3061 tctcagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3121 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3181 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3241 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3301 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3361 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3421 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3481 cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc gcaattcgta
     3541 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3601 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3661 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3721 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3781 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3841 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3961 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4021 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4081 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4141 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4201 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4261 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4321 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4381 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4441 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4501 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4561 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4621 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4681 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4741 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4801 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4861 ccggcaccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4921 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4981 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5041 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5101 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5161 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5221 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5281 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5341 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5401 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5461 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5521 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5581 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5641 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     5701 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5761 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5821 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5881 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5941 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6001 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6061 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6121 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     6181 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     6241 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     6301 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctccaccgcg
     6361 gtg
//
