LOCUS       sequence-406239-       10130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-tagged human integrin beta-4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Zhang W, Lu CH, Nakamoto ML, Tsai CT, Roy AR, Lee CE, Yang Y, Jahed 
            Z, Li X, Cui B
  TITLE     Curved adhesions mediate cell attachment to soft matrix fibres in 
            three dimensions.
  JOURNAL   Nat Cell Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1.
  PUBMED    37770566
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1."
COMMENT     Sequence Label: Integrin beta-4-GFP
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6070..6079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6076..6078,6079..6081,6082..6795)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6076..6078,6079..6081,6082..6792)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6121..6142)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6382..6401)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6729..6750
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6918..7039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6955..6974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7009..7028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7046..7501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7183..7204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7395..7414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7528..7632
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7634..7991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7634..7825
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7657..7677)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7842..7977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8026..8820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8026..8820
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8080..8099)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8690..8709
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9008..9027)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9052..9099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9428..10016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9917..9936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgttt
      601 aaacgggccc tctagactcg agcggccgcc actgtgctgg atatctgcag aattcggcgc
      661 ccgcgcgctg cagccccatc tcctagcggc agcccaggcg cggagggagc gagtccgccc
      721 cgaggtaggt ccaggacggg cgcacagcag cagccgaggc tggccgggag agggaggaag
      781 aggatggcag ggccacgccc cagcccatgg gccaggctgc tcctggcagc cttgatcagc
      841 gtcagcctct ctgggacctt ggcaaaccgc tgcaagaagg ccccagtgaa gagctgcacg
      901 gagtgtgtcc gtgtggataa ggactgcgcc tactgcacag acgagatgtt cagggaccgg
      961 cgctgcaaca cccaggcgga gctgctggcc gcgggctgcc agcgggagag catcgtggtc
     1021 atggagagca gcttccaaat cacagaggag acccagattg acaccaccct gcggcgcagc
     1081 cagatgtccc cccaaggcct gcgggtccgt ctgcggcccg gtgaggagcg gcattttgag
     1141 ctggaggtgt ttgagccact ggagagcccc gtggacctgt acatcctcat ggacttctcc
     1201 aactccatgt ccgatgatct ggacaacctc aagaagatgg ggcagaacct ggctcgggtc
     1261 ctgagccagc tcaccagcga ctacactatt ggatttggca agtttgtgga caaagtcagc
     1321 gtcccgcaga cggacatgag gcctgagaag ctgaaggagc cctggcccaa cagtgacccc
     1381 cccttctcct tcaagaacgt catcagcctg acagaagatg tggatgagtt ccggaataaa
     1441 ctgcagggag agcggatctc aggcaacctg gatgctcctg agggcggctt cgatgccatc
     1501 ctgcagacag ctgtgtgcac gagggacatt ggctggcgcc cggacagcac ccacctgctg
     1561 gtcttctcca ccgagtcagc cttccactat gaggctgatg gcgccaacgt gctggctggc
     1621 atcatgagcc gcaacgatga acggtgccac ctggacacca cgggcaccta cacccagtac
     1681 aggacacagg actacccgtc ggtgcccacc ctggtgcgcc tgctcgccaa gcacaacatc
     1741 atccccatct ttgctgtcac caactactcc tatagctact acgagaagct tcacacctat
     1801 ttccctgtct cctcactggg ggtgctgcag gaggactcgt ccaacatcgt ggagctgctg
     1861 gaggaggcct tcaatcggat ccgctccaac ctggacatcc gggccctaga cagcccccga
     1921 ggccttcgga cagaggtcac ctccaagatg ttccagaaga cgaggactgg gtcctttcac
     1981 atccggcggg gggaagtggg tatataccag gtgcagctgc gggcccttga gcacgtggat
     2041 gggacgcacg tgtgccagct gccggaggac cagaagggca acatccatct gaaaccttcc
     2101 ttctccgacg gcctcaagat ggacgcgggc atcatctgtg atgtgtgcac ctgcgagctg
     2161 caaaaagagg tgcggtcagc tcgctgcagc ttcaacggag acttcgtgtg cggacagtgt
     2221 gtgtgcagcg agggctggag tggccagacc tgcaactgct ccaccggctc tctgagtgac
     2281 attcagccct gcctgcggga gggcgaggac aagccgtgct ccggccgtgg ggagtgccag
     2341 tgcgggcact gtgtgtgcta cggcgaaggc cgctacgagg gtcagttctg cgagtatgac
     2401 aacttccagt gtccccgcac ttccgggttc ctctgcaatg accgaggacg ctgctccatg
     2461 ggccagtgtg tgtgtgagcc tggttggaca ggcccaagct gtgactgtcc cctcagcaat
     2521 gccacctgca tcgacagcaa tgggggcatc tgtaatggac gtggccactg tgagtgtggc
     2581 cgctgccact gccaccagca gtcgctctac acggacacca tctgcgagat caactactcg
     2641 gcgatccacc cgggcctctg cgaggaccta cgctcctgcg tgcagtgcca ggcgtggggc
     2701 accggcgaga agaaggggcg cacgtgtgag gaatgcaact tcaaggtcaa gatggtggac
     2761 gagcttaaga gagccgagga ggtggtggtg cgctgctcct tccgggacga ggatgacgac
     2821 tgcacctaca gctacaccat ggaaggtgac ggcgcccctg ggcccaacag cactgtcctg
     2881 gtgcacaaga agaaggactg ccctccgggc tccttctggt ggctcatccc cctgctcctc
     2941 ctcctcctgc cgctcctggc cctgctactg ctgctatgct ggaagtactg tgcctgctgc
     3001 aaggcctgcc tggcacttct cccgtgctgc aaccgaggtc acatggtggg ctttaaggaa
     3061 gaccactaca tgctgcggga gaacctgatg gcctctgacc acttggacac gcccatgctg
     3121 cgcagcggga acctcaaggg ccgtgacgtg gtccgctgga aggtcaccaa caacatgcag
     3181 cggcctggct ttgccactca tgccgccagc atcaacccca cagagctggt gccctacggg
     3241 ctgtccttgc gcctggcccg cctttgcacc gagaacctgc tgaagcctga cactcgggag
     3301 tgcgcccagc tgcgccagga ggtggaggag aacctgaacg aggtctacag gcagatctcc
     3361 ggtgtacaca agctccagca gaccaagttc cggcagcagc ccaatgccgg gaaaaagcaa
     3421 gaccacacca ttgtggacac agtgctgatg gcgccccgct cggccaagcc ggccctgctg
     3481 aagcttacag agaagcaggt ggaacagagg gccttccacg acctcaaggt ggcccccggc
     3541 tactacaccc tcactgcaga ccaggacgcc cggggcatgg tggagttcca ggagggcgtg
     3601 gagctggtgg acgtacgggt gcccctcttt atccggcctg aggatgacga cgagaagcag
     3661 ctgctggtgg aggccatcga cgtgcccgca ggcactgcca ccctcggccg ccgcctggta
     3721 aacatcacca tcatcaagga gcaagccaga gacgtggtgt cctttgagca gcctgagttc
     3781 tcggtcagcc gcggggacca ggtggcccgc atccctgtca tccggcgtgt cctggacggc
     3841 gggaagtccc aggtctccta ccgcacacag gatggcaccg cgcagggcaa ccgggactac
     3901 atccccgtgg agggtgagct gctgttccag cctggggagg cctggaaaga gctgcaggtg
     3961 aagctcctgg agctgcaaga agttgactcc ctcctgcggg gccgccaggt ccgccgtttc
     4021 cacgtccagc tcagcaaccc taagtttggg gcccacctgg gccagcccca ctccaccacc
     4081 atcatcatca gggacccaga tgaactggac cggagcttca cgagtcagat gttgtcatca
     4141 cagccacccc ctcacggcga cctgggcgcc ccgcagaacc ccaatgctaa ggccgctggg
     4201 tccaggaaga tccatttcaa ctggctgccc ccttctggca agccaatggg gtacagggta
     4261 aagtactgga ttcagggtga ctccgaatcc gaagcccacc tgctcgacag caaggtgccc
     4321 tcagtggagc tcaccaacct gtacccgtat tgcgactatg agatgaaggt gtgcgcctac
     4381 ggggctcagg gcgagggacc ctacagctcc ctggtgtcct gccgcaccca ccaggaagtg
     4441 cccagcgagc cagggcgtct ggccttcaat gtcgtctcct ccacggtgac ccagctgagc
     4501 tgggctgagc cggctgagac caacggtgag atcacagcct acgaggtctg ctatggcctg
     4561 gtcaacgatg acaaccgacc tattgggccc atgaagaaag tgctggttga caaccctaag
     4621 aaccggatgc tgcttattga gaaccttcgg gagtcccagc cctaccgcta cacggtgaag
     4681 gcgcgcaacg gggccggctg ggggcctgag cgggaggcca tcatcaacct ggccacccag
     4741 cccaagaggc ccatgtccat ccccatcatc cctgacatcc ctatcgtgga cgcccagagc
     4801 ggggaggact acgacagctt ccttatgtac agcgatgacg ttctacgctc tccatcgggc
     4861 agccagaggc ccagcgtctc cgatgacact gagcacctgg tgaatggccg gatggacttt
     4921 gccttcccgg gcagcaccaa ctccctgcac aggatgacca cgaccagtgc tgctgcctat
     4981 ggcacccacc tgagcccaca cgtgccccac cgcgtgctaa gcacatcctc caccctcaca
     5041 cgggactaca actcactgac ccgctcagaa cactcacact cgaccacact gccgagggac
     5101 tactccaccc tcacctccgt ctcctcccac gactctcgcc tgactgctgg tgtgcccgac
     5161 acgcccaccc gcctggtgtt ctctgccctg gggcccacat ctctcagagt gagctggcag
     5221 gagccgcggt gcgagcggcc gctgcagggc tacagtgtgg agtaccagct gctgaacggc
     5281 ggtgagctgc atcggctcaa catccccaac cctgcccaga cctcggtggt ggtggaagac
     5341 ctcctgccca accactccta cgtgttccgc gtgcgggccc agagccagga aggctggggc
     5401 cgagagcgtg agggtgtcat caccattgaa tcccaggtgc acccgcagag cccactgtgt
     5461 cccctgccag gctccgcctt cactttgagc actcccagtg ccccaggccc gctggtgttc
     5521 actgccctga gcccagactc gctgcagctg agctgggagc ggccacggag gcccaatggg
     5581 gatatcgtcg gctacctggt gacctgtgag atggcccaag gaggagggcc agccaccgca
     5641 ttccgggtgg atggagacag ccccgagagc cggctgaccg tgccgggcct cagcgagaac
     5701 gtgccctaca agttcaaggt gcaggccagg accactgagg gcttcgggcc agagcgcgag
     5761 ggcatcatca ccatagagtc ccaggatgga ggacccttcc cgcagctggg cagccgtgcc
     5821 gggctcttcc agcacccgct gcaaagcgag tacagcagca tcaccaccac ccacaccagc
     5881 gccaccgagc ccttcctagt ggatgggccg accctggggg cccagcacct ggaggcaggc
     5941 ggctccctca cccggcatgt gacccaggag tttgtgagcc ggacactgac caccagcgga
     6001 acccttagca cccacatgga ccaacagttc ttccaaactt cggtaccgcg ggcccgggat
     6061 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     6121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     6181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     6241 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     6301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     6361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     6481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     6541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     6601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6661 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     6721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     6781 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     6841 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6901 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7021 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     7081 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     7141 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7201 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     7261 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     7321 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     7381 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     7441 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7501 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7621 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     7681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7741 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7801 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7861 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7921 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7981 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     8041 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     8101 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     8161 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     8221 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     8281 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     8341 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     8401 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     8461 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     8521 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     8581 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     8641 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     8701 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     8761 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     8821 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     8881 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8941 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     9001 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     9061 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     9121 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     9181 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     9241 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     9301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
