LOCUS       sequence-406373-        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector containing AAV2 rep and AM cap and partial AAV2
            p5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Gonzalez-Sandoval A, Pekrun K, Tsuji S, Zhang F, Hung KL, Chang HY, 
            Kay MA
  TITLE     The AAV capsid can influence the epigenetic marking of rAAV 
            delivered episomal genomes in a species dependent manner.
  JOURNAL   Nat Commun. 2023 Apr 28;14(1):2448. doi: 10.1038/s41467-023-38106-3.
  PUBMED    37117181
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-28"; volume: "14(1):2448. doi"; pages: " 
            10.1038/s41467-023-38106-3"
COMMENT     Sequence Label: AAV-AM
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3943..3954
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4406..4426)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4406..4424)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4445..4461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4445..4461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4458..4480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4469..4485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4493..4499,4500..4517,4518..4523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4538..4559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4676..4693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4847..5435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4927..4946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5606..6397,6398..6466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6229..6248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6467..6571)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6597..7052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6734..6755)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6946..6965
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7179..7201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7193..7210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7194..7210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7220..7239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7220..7238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7267..7283
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7268..7284
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
       61 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
      121 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
      181 tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gctcttttct
      241 ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg
      301 gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga
      361 gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa
      421 atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc
      481 ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct
      541 gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga
      601 cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa
      661 aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg
      721 agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
      781 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
      841 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
      901 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
      961 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     1021 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     1081 ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg
     1141 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     1201 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     1261 cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     1321 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     1381 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     1441 aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     1501 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     1561 acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg
     1621 gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata
     1681 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1741 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     1801 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     1861 ttgaacaata aatgatttaa atcaggtatg gctgctgacg gttatcttcc agattggctc
     1921 gaggacaacc tttctgaagg cattcgagag tggtgggcgc tgcaacctgg agcccctaaa
     1981 cccaaggcaa atcaacaaca tcaggacaac gctcggggtc ttgtgcttcc gggttacaaa
     2041 tacctcggac ccggcaacgg actcgacaag ggggaacccg tcaacgcagc ggacgcggca
     2101 gccctcgagc acgacaaggc ctacgaccag cagctcaagg ccggtgacaa cccctacctc
     2161 aagtacaacc acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg
     2221 ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg
     2281 gttgaggaag cggctaagac ggctcctgga aagaagaggc ctgtagatca gtctcctcag
     2341 gaaccggact catcatctgg tgttggcaaa tcgggcaaac agcctgccag aaaaagacta
     2401 aatttcggtc agactggcga ctcagagtca gtcccagacc ctcaacctct cggagaacca
     2461 ccagcagccc ccacaagttt gggatctaat acaatggctt caggcggtgg cgcaccaatg
     2521 gcagacaata acgagggtgc cgatggagtg ggtaattcct caggaaattg gcattgcgat
     2581 tcccaatggc tgggcgacag agtcatcacc accagcacca gaacctgggc cctgcccact
     2641 tacaacaacc atctctacaa gcaaatctcc agccaatcag gaggagcttc aaacgacaac
     2701 cactactttg gctacagcac cccttggggg tattttgact ttaacagatt ccactgccac
     2761 ttctcaccac gtgactggca gcgactcatt aacaacaact ggggattccg gcccaagaaa
     2821 ctcagcttca agctcttcaa catccaagtt aaagaggtca cgcagaacga tggcacgacg
     2881 actattgcca ataaccttac cagcacggtt caagtgttta cggactcgga gtatcagctc
     2941 ccgtacgtgc tcgggtcggc gcaccaaggc tgtctcccgc cgtttccagc ggacgtcttc
     3001 atggtccctc agtatggata cctcaccctg aacaacggaa gtcaagcggt gggacgctca
     3061 tccttttact gcctggagta cttcccttcg cagatgctaa ggactggaaa taacttccaa
     3121 ttcagctata ccttcgagga tgtacctttt cacagcagct acgctcacag ccagagtttg
     3181 gatcgcttga tgaatcctct tattgatcag tatctgtact acctgaacag aacgcaagga
     3241 acaacctctg gaacaaccaa ccaatcacgg ctgcttttta gccaggctgg gcctcagtct
     3301 atgtctttgc aggccagaaa ttggctacct gggccctgct accggcaaca gagactttca
     3361 aagactgcta acgacaacaa caacagtaac tttccttgga cagcggccag caaatatcat
     3421 ctcaatggcc gcgactcgct ggtgaatcca ggaccagcta tggccagtca caaggacgat
     3481 gaagaaaaat ttttccctat gcacggcaat ctaatatttg gcaaagaagg gacaacggca
     3541 agtaacgcag aattagataa tgtaatgatt acggatgaag aagagattcg taccaccaat
     3601 cctgtggcaa cagagcagta tggaactgtg gcaaataact tgcagagctc aaatacagct
     3661 cccacgacta gaactgtcaa tgatcagggg gccttacctg gcatggtgtg gcaagatcgt
     3721 gacgtgtacc ttcaaggacc tatctgggca aagattcctc acacggatgg acactttcat
     3781 ccttctcctc tgatgggagg ctttggactg aaacatccgc ctcctcaaat catgatcaaa
     3841 aatactccgg taccggcaaa tcctccgacg actttcagcc cggccaagtt tgcttcattt
     3901 atcactcagt actccactgg acaggtcagc gtggaaattg agtgggagct acagaaagaa
     3961 aacagcaaac gttggaatcc agagattcag tacacttcca actacaacaa gtctgttaat
     4021 gtggacttta ctgtagacac taatggtgtt tatagtgaac ctcgccccat tggcacccgt
     4081 taccttaccc gtcccctgta attgcctgtt aatcaataaa ccggttgatt cgtttcagtt
     4141 gaactttggt ctctgcgaag ggcgaattcg tttaaacctg caggactaga ggtcctgtat
     4201 tagaggtcac gtgagtgttt tgcgacattt tgcgacacca tgtggtcacg ctgggtattt
     4261 aagcccgagt gagcacgcag ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc
     4321 caagccgaat tctgcagata tccatcacac tggcggccgc tcgactagag cggccgccac
     4381 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     4441 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4501 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4561 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4621 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4681 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4741 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4801 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4861 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4921 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4981 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5041 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5101 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5161 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5221 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5281 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5341 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5401 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5461 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5521 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5581 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5641 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5701 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5761 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5821 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5881 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5941 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6001 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6061 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6121 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6181 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6241 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6301 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6361 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6421 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6481 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6541 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     6601 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6661 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6721 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6781 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     6841 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     6901 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     6961 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7021 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     7081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7141 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7201 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
     7261 cctcgatcga ggtcgacggt atcgggggag ctcgcagggt ctccattttg aagcgggagg
     7321 tttgaacgcg cag
//
