LOCUS       sequence-406374-        7330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector containing AAV2 rep and LK03 cap and partial 
            AAV2 p5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Lisowski L, Dane AP, Chu K, Zhang Y, Cunningham SC, Wilson EM, 
            Nygaard S, Grompe M, Alexander IE, Kay MA
  TITLE     Selection and evaluation of clinically relevant AAV variants in a 
            xenograft liver model.
  JOURNAL   Nature. 2014 Feb 20;506(7488):382-6. doi: 10.1038/nature12875. Epub 
            2013 Dec 25.
  PUBMED    24390344
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12875"; journalName: "Nature"; date: "2014-02-20- 
            20"; volume: "506"; issue: "7488"; pages: "382-6"
COMMENT     Sequence Label: AAV-LK03
FEATURES             Location/Qualifiers
     source          1..7330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3940..3951
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4403..4423)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4403..4421)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4442..4458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4442..4458)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4455..4477)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4466..4482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4490..4496,4497..4514,4515..4520))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4535..4556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4673..4690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4844..5432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4924..4943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5603..6394,6395..6463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6226..6245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6464..6568)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6594..7049)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6731..6752)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6943..6962
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7176..7198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7190..7207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7191..7207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7217..7236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7217..7235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7264..7280
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7265..7281
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
       61 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
      121 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
      181 tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gctcttttct
      241 ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg
      301 gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga
      361 gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa
      421 atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc
      481 ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct
      541 gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga
      601 cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa
      661 aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg
      721 agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
      781 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
      841 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
      901 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
      961 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     1021 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     1081 ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg
     1141 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     1201 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     1261 cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     1321 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     1381 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     1441 aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     1501 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     1561 acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg
     1621 gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata
     1681 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1741 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     1801 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     1861 ttgaacaata aatgatttaa atcaggtatg gctgctgacg gttatcttcc agattggctc
     1921 gaggacaacc tttctgaagg cattcgagag tggtgggcgc tgcaacctgg agcccctaaa
     1981 cccaaggcaa atcaacaaca tcaggacaac gctcggggtc ttgtgcttcc gggttacaaa
     2041 tacctcggac ccggcaacgg actcgacaag ggggaacccg tcaacgcagc ggacgcggca
     2101 gccctcgagc acgacaaggc ctacgaccag cagctcaagg ccggtgacaa cccctacctc
     2161 aagtacaacc acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg
     2221 ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg
     2281 gttgaggaag cggctaagac ggctcctgga aagaagaggc ctgtagatca gtctcctcag
     2341 gaaccggact catcatctgg tgttggcaaa tcgggcaaac agcctgccag aaaaagacta
     2401 aatttcggtc agactggcga ctcagagtca gtcccagacc ctcaacctct cggagaacca
     2461 ccagcagccc ccacaagttt gggatctaat acaatggctt caggcggtgg cgcaccaatg
     2521 gcagacaata acgagggtgc cgatggagtg ggtaattcct caggaaattg gcattgcgat
     2581 tcccaatggc tgggcgacag agtcatcacc accagcacca gaacctgggc cctgcccact
     2641 tacaacaacc atctctacaa gcaaatctcc agccaatcag gagcttcaaa cgacaaccac
     2701 tactttggct acagcacccc ttgggggtat tttgacttta acagattcca ctgccacttc
     2761 tcaccacgtg actggcagcg actcattaac aacaactggg gattccggcc caagaaactc
     2821 agcttcaagc tcttcaacat ccaagttaaa gaggtcacgc agaacgatgg cacgacgact
     2881 attgccaata accttaccag cacggttcaa gtgtttacgg actcggagta tcagctcccg
     2941 tacgtgctcg ggtcggcgca ccaaggctgt ctcccgccgt ttccagcgga cgtcttcatg
     3001 gtccctcagt atggatacct caccctgaac aacggaagtc aagcggtggg acgctcatcc
     3061 ttttactgcc tggagtactt cccttcgcag atgctaagga ctggaaataa cttccaattc
     3121 agctatacct tcgaggatgt accttttcac agcagctacg ctcacagcca gagtttggat
     3181 cgcttgatga atcctcttat tgatcagtat ctgtactacc tgaacagaac gcaaggaaca
     3241 acctctggaa caaccaacca atcacggctg ctttttagcc aggctgggcc tcagtctatg
     3301 tctttgcagg ccagaaattg gctacctggg ccctgctacc ggcaacagag actttcaaag
     3361 actgctaacg acaacaacaa cagtaacttt ccttggacag cggccagcaa atatcatctc
     3421 aatggccgcg actcgctggt gaatccagga ccagctatgg ccagtcacaa ggacgatgaa
     3481 gaaaaatttt tccctatgca cggcaatcta atatttggca aagaagggac aacggcaagt
     3541 aacgcagaat tagataatgt aatgattacg gatgaagaag agattcgtac caccaatcct
     3601 gtggcaacag agcagtatgg aactgtggca aataacttgc agagctcaaa tacagctccc
     3661 acgactagaa ctgtcaatga tcagggggcc ttacctggca tggtgtggca agatcgtgac
     3721 gtgtaccttc aaggacctat ctgggcaaag attcctcaca cggatggaca ctttcatcct
     3781 tctcctctga tgggaggctt tggactgaaa catccgcctc ctcaaatcat gatcaaaaat
     3841 actccggtac cggcaaatcc tccgacgact ttcagcccgg ccaagtttgc ttcatttatc
     3901 actcagtact ccactggaca ggtcagcgtg gaaattgagt gggagctaca gaaagaaaac
     3961 agcaaacgtt ggaatccaga gattcagtac acttccaact acaacaagtc tgttaatgtg
     4021 gactttactg tagacactaa tggtgtttat agtgaacctc gccccattgg cacccgttac
     4081 cttacccgtc ccctgtaatt gcctgttaat caataaaccg gttgattcgt ttcagttgaa
     4141 ctttggtctc tgcgaagggc gaattcgttt aaacctgcag gactagaggt cctgtattag
     4201 aggtcacgtg agtgttttgc gacattttgc gacaccatgt ggtcacgctg ggtatttaag
     4261 cccgagtgag cacgcagggt ctccattttg aagcgggagg tttgaacgcg cagccgccaa
     4321 gccgaattct gcagatatcc atcacactgg cggccgctcg actagagcgg ccgccaccgc
     4381 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     4441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     6601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6661 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6721 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6781 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     6841 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6901 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6961 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7021 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     7081 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7141 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7201 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct
     7261 cgatcgaggt cgacggtatc gggggagctc gcagggtctc cattttgaag cgggaggttt
     7321 gaacgcgcag
//
