LOCUS       sequence-406379-       11200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing the protein DHX36 fused to mCherry in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Danino YM, Molitor L, Rosenbaum-Cohen T, Kaiser S, Cohen Y, Porat Z,
            Marmor-Kollet H, Katina C, Savidor A, Rotkopf R, Ben-Isaac E, Golani
            O, Levin Y, Monchaud D, Hickson ID, Hornstein E
  TITLE     BLM helicase protein negatively regulates stress granule formation 
            through unwinding RNA G-quadruplex structures.
  JOURNAL   Nucleic Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613.
  PUBMED    37503837
REFERENCE   2  (bases 1 to 11200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613."
COMMENT     Sequence Label: pUltraHot-mCherry-DHX36-iso1-WT
FEATURES             Location/Qualifiers
     source          1..11200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2022..3233
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3070..3089
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3266..3275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3272..3979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3417..3435)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3685..3704
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7047..7635
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7100..7120)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7518..7529)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7726..7959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8031..8152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8068..8087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8122..8141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8192..8327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8347..8366)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8348..8366)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8376..8393)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8376..8392)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8534..8989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8621..8640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8831..8852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9015..9119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9120..9188,9189..9980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9338..9357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10151..10739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10640..10659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10893..10910
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11027..11048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11063..11068,11069..11086,11087..11093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11101..11117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11106..11128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11125..11141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11125..11141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11160..11180
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11162..11180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2041 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2101 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2161 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2221 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2281 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2341 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     2401 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     2461 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     2521 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     2581 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     2641 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     2701 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     2761 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     2821 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     2881 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     2941 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3001 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3061 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3121 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3181 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3241 gggctgcagg tccgatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     3301 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3361 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3421 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3481 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3541 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3601 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3661 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3721 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3781 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3841 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3901 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3961 catggacgag ctgtacaaga gttatgacta ccatcagaac tggggccgtg atgggggtcc
     4021 ccgcagctcc ggtgggggct atggaggggg gccagcaggg ggtcatggag gtaaccgagg
     4081 ctccggagga ggcggcggcg gcggaggggg tggtcgaggc ggcaggggcc ggcatcccgg
     4141 gcacctgaaa ggccgcgaaa tcggcatgtg gtacgcgaaa aaacaggggc agaagaacaa
     4201 ggaagcggag aggcaagaga gagctgtagt acacatggat gaacgacgag aagaacaaat
     4261 tgtacagtta ctgaattctg ttcaagcgaa gaatgataaa gagtcagaag cacagatatc
     4321 ctggtttgct cctgaggatc atggatacgg tactgaagtt tctactaaga acacaccatg
     4381 ctcagagaac aaacttgaca tccaggaaaa gaagttgata aatcaagaaa aaaaaatgtt
     4441 tagaatcagg aacagatcat atattgaccg agattctgag tatctcttgc aagaaaatga
     4501 accagatgga actttagacc aaaaattatt ggaagattta caaaagaaaa aaaatgacct
     4561 tcggtatatt gaaatgcagc atttcagaga aaagctgcct tcgtatggaa tgcaaaagga
     4621 attggtaaat ttaattgata accatcaggt aacagtaata agtggtgaaa ctggttgtgg
     4681 caaaaccact caagttactc agttcatttt ggataactac attgaaagag gaaaaggatc
     4741 tgcttgcaga atagtttgta ctcagccaag aagaattagt gccatttcag ttgcggaaag
     4801 agtagctgca gaaagggcag aatcttgtgg cagtggtaat agtactggat atcaaattcg
     4861 tctccagagt cggttgccaa ggaaacaggg ttctatctta tactgtacaa caggaatcat
     4921 ccttcagtgg ctccagtcag acccgtattt gtccagtgtt agtcatatcg tacttgatga
     4981 aatccatgaa agaaatctgc agtcagatgt tttaatgact gttgttaaag accttctcaa
     5041 ttttcgatct gacttgaaag taatattgat gagtgcaaca ttgaatgcag aaaagttttc
     5101 agaatatttt ggtaactgtc caatgataca tatacctggt tttacctttc cggttgtgga
     5161 atatcttttg gaagatgtaa ttgaaaaaat aaggtatgtt ccagaacaaa aagaacacag
     5221 atcccagttt aagaggggtt tcatgcaagg gcatgtaaat agacaagaaa aagaagaaaa
     5281 agaagcaata tataaagaac gttggccaga ttatgtaagg gaactgcgaa gaaggtattc
     5341 tgcaagtact gtagatgtta tagaaatgat ggaggatgat aaagttgatc tgaatttgat
     5401 tgttgccctc atccgataca ttgttttgga agaagaggat ggtgcgatac tggtctttct
     5461 gccaggctgg gacaatatca gcactttaca tgatctcttg atgtcacaag taatgtttaa
     5521 atcagataaa tttttaatta tacctttaca ttcactgatg cctacagtta accagacaca
     5581 ggtgtttaaa agaacccctc ctggtgttcg gaaaatagta attgctacca acattgcgga
     5641 gactagcatt accatagatg atgtcgttta tgtgatagat ggaggaaaaa taaaagagac
     5701 gcattttgat actcagaaca atatcagtac aatgtccgct gagtgggtta gtaaagctaa
     5761 tgccaaacag agaaaaggtc gagctggaag agttcaacct ggtcattgct atcatctgta
     5821 taatggtctt agagcaagtc ttctagatga ctatcaactg ccagaaattt tgagaactcc
     5881 tttggaagaa ctttgtttac aaataaagat tttaaggcta ggtggaattg cttattttct
     5941 gagtagatta atggacccac catcaaatga ggcagtgtta ctctccataa gacacctgat
     6001 ggagctgaac gctttggata aacaagaaga attgacacct cttggagtcc acttggcacg
     6061 attacccgtt gagccacata ttggaaaaat gattcttttt ggagcactgt tctgctgctt
     6121 agacccagta ctcactattg ctgctagtct cagtttcaaa gatccatttg tcattccact
     6181 gggaaaagaa aagattgcag atgcaagaag aaaggaattg gcaaaggata ctagaagtga
     6241 tcacttaaca gttgtgaatg cgtttgaggg ctgggaagag gctaggcgac gtggtttcag
     6301 atacgaaaag gactattgct gggaatattt tctgtcttca aacacactgc agatgctgca
     6361 taacatgaaa ggacagtttg ctgagcatct tcttggagct ggatttgtaa gcagtagaaa
     6421 tcctaaagat ccagaatcta atataaattc agataatgag aagataatta aagctgtcat
     6481 ctgtgctggt ttatatccca aagttgctaa aattcgacta aatttgggta aaaaaagaaa
     6541 aatggtaaaa gtttacacaa aaaccgatgg cctggttgct gttcatccta aatctgttaa
     6601 tgtggagcaa acagactttc actacaactg gcttatctat cacctaaaga tgagaacaag
     6661 cagtatatac ttgtatgact gcacagaggt ttccccatac tgtctcttgt tttttggagg
     6721 tgacatttcc atccagaagg ataacgatca ggaaactatt gctgtagatg agtggattgt
     6781 atttcagtct ccagcaagaa ttgcccatct tgttaaggaa ttaagaaagg aactagatat
     6841 tcttctgcaa gagaagattg aaagtcctca tcctgtagac tggaatgaca ctaaatccag
     6901 agactgtgca gtactgtcag ctattataga cttgatcaaa acacaggaaa aggcaactcc
     6961 caggaacttt ccgccacgat tccaggatgg atattacagc tgagctagcg tgtgatcaga
     7021 attcgttccg gagtcgtcga ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     7081 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7141 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7201 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7261 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7321 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7381 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7441 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7501 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7561 cggctctgcg gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7621 gggccgcctc cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca
     7681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctacgta
     7741 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8161 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8341 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     8401 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8461 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8521 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8761 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8821 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8881 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8941 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10261 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10681 acctctcact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10981 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11041 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11101 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11161 caattaaccc tcactaaagg gaacaaaagc tggagctgca
//
