LOCUS       sequence-406382-        2550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector used for high-throughput cloning and subsequent transfection 
            of MPRA libraries into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Oliveros W, Delfosse K, Lato DF, Kiriakopulos K, Mokhtaridoost M, 
            Said A, McMurray BJ, Browning JWL, Mattioli K, Meng G, Ellis J, 
            Mital S, Mele M, Maass PG
  TITLE     Systematic characterization of regulatory variants of blood pressure
            genes.
  JOURNAL   Cell Genom. 2023 May 24;3(7):100330. doi: 
            10.1016/j.xgen.2023.100330. eCollection 2023 Jul 12.
  PUBMED    37492106
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2023-05-24"; volume: "3(7):100330. doi"; pages: " 
            10.1016/j.xgen.2023.100330. eCollection 2023 Jul 12"
COMMENT     Sequence Label: MPRA Empty Vector
FEATURES             Location/Qualifiers
     source          1..2550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    104..225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(115..134)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     169..188
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(473..490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(644..1232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(724..743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1432..2223,2224..2292))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2397..2445
                     /note="synthetic polyadenylation signal"
     misc_feature    2459..2550
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2499..2518
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
      121 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      181 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      241 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      301 ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa
      361 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
      421 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
      481 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      541 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      781 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1081 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1321 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1381 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     1441 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     1501 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     1561 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     1621 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     1681 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     1741 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     1801 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     1861 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     1921 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     1981 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     2041 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     2101 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     2161 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     2221 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     2281 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     2341 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     2401 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     2461 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     2521 gtgcaagtgc aggtgccaga acatttctct
//
