LOCUS       sequence-406398-       12427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing the protein BLM fused to mCherry in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Danino YM, Molitor L, Rosenbaum-Cohen T, Kaiser S, Cohen Y, Porat Z,
            Marmor-Kollet H, Katina C, Savidor A, Rotkopf R, Ben-Isaac E, Golani
            O, Levin Y, Monchaud D, Hickson ID, Hornstein E
  TITLE     BLM helicase protein negatively regulates stress granule formation 
            through unwinding RNA G-quadruplex structures.
  JOURNAL   Nucleic Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613.
  PUBMED    37503837
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613."
COMMENT     Sequence Label: pUltraHot-mCherry-BLM-WT
FEATURES             Location/Qualifiers
     source          1..12427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2022..3233
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3070..3089
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3266..3275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3272..3979
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3417..3435)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3685..3704
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8274..8862
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8327..8347)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8745..8756)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8953..9186
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9258..9379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9295..9314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9349..9368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9419..9554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9574..9593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9575..9593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9603..9620)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9603..9619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9612..9634)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9761..10216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9848..9867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10058..10079
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10242..10346
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10347..10415,10416..11207)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10565..10584)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11378..11966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11867..11886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12120..12137
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12254..12275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12290..12295,12296..12313,12314..12320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12328..12344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12333..12355
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12352..12368
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12352..12368
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12387..12407
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12389..12407
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2041 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2101 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2161 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2221 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2281 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2341 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     2401 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     2461 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     2521 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     2581 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     2641 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     2701 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     2761 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     2821 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     2881 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     2941 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3001 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3061 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3121 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3181 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3241 gggctgcagg tccgatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     3301 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3361 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3421 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3481 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3541 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3601 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3661 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3721 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3781 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3841 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3901 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3961 catggacgag ctgtacaagg ctgctgttcc tcaaaataat ctacaggagc aactagaacg
     4021 tcactcagcc agaacactta ataataaatt aagtctttca aaaccaaaat tttcaggttt
     4081 cacttttaaa aagaaaacat cttcagataa caatgtatct gtaactaatg tgtcagtagc
     4141 aaaaacacct gtattaagaa ataaagatgt taatgttacc gaagactttt ccttcagtga
     4201 acctctaccc aacaccacaa atcagcaaag ggtcaaggac ttctttaaaa atgctccagc
     4261 aggacaggaa acacagagag gtggatcaaa atcattattg ccagatttct tgcagactcc
     4321 gaaggaagtt gtatgcacta cccaaaacac accaactgta aagaaatccc gggatactgc
     4381 tctcaagaaa ttagaattta gttcttcacc agattcttta agtaccatca atgattggga
     4441 tgatatggat gactttgata cttctgagac ttcaaaatca tttgttacac caccccaaag
     4501 tcactttgta agagtaagca ctgctcagaa atcaaaaaag ggtaagagaa acttttttaa
     4561 agcacagctt tatacaacaa acacagtaaa gactgatttg cctccaccct cctctgaaag
     4621 cgagcaaata gatttgactg aggaacagaa ggatgactca gaatggttaa gcagcgatgt
     4681 gatttgcatc gatgatggcc ccattgctga agtgcatata aatgaagatg ctcaggaaag
     4741 tgactctctg aaaactcatt tggaagatga aagagataat agcgaaaaga agaagaattt
     4801 ggaagaagct gaattacatt caactgagaa agttccatgt attgaatttg atgatgatga
     4861 ttatgatacg gattttgttc caccttctcc agaagaaatt atttctgctt cttcttcctc
     4921 ttcaaaatgc cttagtacgt taaaggacct tgacacatct gacagaaaag aggatgttct
     4981 tagcacatca aaagatcttt tgtcaaaacc tgagaaaatg agtatgcagg agctgaatcc
     5041 agaaaccagc acagactgtg acgctagaca gataagttta cagcagcagc ttattcatgt
     5101 gatggagcac atctgtaaat taattgatac tattcctgat gataaactga aacttttgga
     5161 ttgtgggaac gaactgcttc agcagcggaa cataagaagg aaacttctaa cggaagtaga
     5221 ttttaataaa agtgatgcca gtcttcttgg ctcattgtgg agatacaggc ctgattcact
     5281 tgatggccct atggagggtg attcctgccc tacagggaat tctatgaagg agttaaattt
     5341 ttcacacctt ccctcaaatt ctgtttctcc tggggactgt ttactgacta ccaccctagg
     5401 aaagacagga ttctctgcca ccaggaagaa tctttttgaa aggcctttat tcaataccca
     5461 tttacagaag tcctttgtaa gtagcaactg ggctgaaaca ccaagactag gaaaaaaaaa
     5521 tgaaagctct tatttcccag gaaatgttct cacaagcact gctgtgaaag atcagaataa
     5581 acatactgct tcaataaatg acttagaaag agaaacccaa ccttcctatg atattgataa
     5641 ttttgacata gatgactttg atgatgatga tgactgggaa gacataatgc ataatttagc
     5701 agccagcaaa tcttccacag ctgcctatca acccatcaag gaaggtcggc caattaaatc
     5761 agtatcagaa agactttcct cagccaagac agactgtctt ccagtgtcat ctactgctca
     5821 aaatataaac ttctcagagt caattcagaa ttatactgac aagtcagcac aaaatttagc
     5881 atccagaaat ctgaaacatg agcgtttcca aagtcttagt tttcctcata caaaggaaat
     5941 gatgaagatt tttcataaaa aatttggcct gcataatttt agaactaatc agctagaggc
     6001 gatcaatgct gcactgcttg gtgaagactg ttttatcctg atgccgactg gaggtggtaa
     6061 gagtttgtgt taccagctcc ctgcctgtgt ttctcctggg gtcactgttg tcatttctcc
     6121 cttgagatca cttatcgtag atcaagtcca aaagctgact tccttggata ttccagctac
     6181 atatctgaca ggtgataaga ctgactcaga agctacaaat atttacctcc agttatcaaa
     6241 aaaagaccca atcataaaac ttctatatgt cactccagaa aagatctgtg caagtaacag
     6301 actcatttct actctggaga atctctatga gaggaagctc ttggcacgtt ttgttattga
     6361 tgaagcacat tgtgtcagtc agtggggaca tgattttcgt caagattaca aaagaatgaa
     6421 tatgcttcgc cagaagtttc cttctgttcc ggtgatggct cttacggcca cagctaatcc
     6481 cagggtacag aaggacatcc tgactcagct gaagattctc agacctcagg tgtttagcat
     6541 gagctttaac agacataatc tgaaatacta tgtattaccg aaaaagccta aaaaggtggc
     6601 atttgattgc ctagaatgga tcagaaagca ccacccatat gattcaggga taatttactg
     6661 cctctccagg cgagaatgtg acaccatggc tgacacgtta cagagagatg ggctcgctgc
     6721 tcttgcttac catgctggcc tcagtgattc tgccagagat gaagtgcagc agaagtggat
     6781 taatcaggat ggctgtcagg ttatctgtgc tacaattgca tttggaatgg ggattgacaa
     6841 accggacgtg cgatttgtga ttcatgcatc tctccctaaa tctgtggagg gttactacca
     6901 agaatctggc agagctggaa gagatgggga aatatctcac tgcctgcttt tctataccta
     6961 tcatgatgtg accagactga aaagacttat aatgatggaa aaagatggaa accatcatac
     7021 aagagaaact cacttcaata atttgtatag catggtacat tactgtgaaa atataacgga
     7081 atgcaggaga atacagcttt tggcctactt tggtgaaaat ggatttaatc ctgatttttg
     7141 taagaaacac ccagatgttt cttgtgataa ttgctgtaaa acaaaggatt ataaaacaag
     7201 agatgtgact gacgatgtga aaagtattgt aagatttgtt caagaacata gttcatcaca
     7261 aggaatgaga aatataaaac atgtaggtcc ttctggaaga tttactatga atatgctggt
     7321 cgacattttc ttggggagta agagtgcaaa aatccagtca ggtatatttg gaaaaggatc
     7381 tgcttattca cgacacaatg ccgaaagact ttttaaaaag ctgatacttg acaagatttt
     7441 ggatgaagac ttatatatca atgccaatga ccaggcgatc gcttatgtga tgctcggaaa
     7501 taaagcccaa actgtactaa atggcaattt aaaggtagac tttatggaaa cagaaaattc
     7561 cagcagtgtg aaaaaacaaa aagcgttagt agcaaaagtg tctcagaggg aagagatggt
     7621 taaaaaatgt cttggagaac ttacagaagt ctgcaaatct ctggggaaag tttttggtgt
     7681 ccattacttc aatattttta ataccgtcac tctcaagaag cttgcagaat ctttatcttc
     7741 tgatcctgag gttttgcttc aaattgatgg tgttactgaa gacaaactgg aaaaatatgg
     7801 tgcggaagtg atttcagtat tacagaaata ctctgaatgg acatcgccag ctgaagacag
     7861 ttccccaggg ataagcctgt ccagcagcag aggccccgga agaagtgccg ctgaggagct
     7921 cgacgaggaa atacctgtat cttcccacta ctttgcaagt aaaaccagaa atgaaaggaa
     7981 gaggaaaaag atgccagcct cccaaaggtc taagaggaga aaaactgctt ccagtggttc
     8041 caaggcaaag ggggggtctg ccacatgtag aaagatatct tccaaaacga aatcctccag
     8101 catcattgga tccagttcag cctcacatac ttctcaagcg acatcaggag ccaatagcaa
     8161 attggggatt atggctccac cgaagcctat aaatagaccg tttcttaagc cttcatatgc
     8221 attctcataa gctagcgtgt gatcagaatt cgttccggag tcgtcgactc gacaatcaac
     8281 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8341 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8401 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8461 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8521 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8581 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     8641 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     8701 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     8761 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc
     8821 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattcgagct
     8881 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8941 aaaagggggg actggaaggg ctacgtaact cccaacgaag acaagatctg ctttttgctt
     9001 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9061 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9121 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9181 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9241 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9361 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9541 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9601 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9661 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9721 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     9781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9901 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9961 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10021 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10081 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10141 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10201 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10261 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10321 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10381 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10441 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10501 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10561 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10621 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10681 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10741 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10801 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10861 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10921 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10981 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11041 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11101 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11161 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11221 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11281 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11341 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11401 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11461 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11521 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11581 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11641 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11701 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11761 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11821 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11881 atctttatag tcctgtcggg tttcgccacc tctcacttga gcgtcgattt ttgtgatgct
    11941 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12001 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12061 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12121 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12241 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12301 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12361 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12421 agctgca
//
