LOCUS       sequence-406406-       11337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DEST vector for MultiSite Gateway assembly and production of 
            recombinant baculovirus using MultiMate assembly. Encodes PE2 under 
            the control of CMV promoter. CRE-Acceptor in MultiBac system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Aulicino F, Pelosse M, Toelzer C, Capin J, Ilegems E, Meysami P, 
            Rollarson R, Berggren PO, Dillingham MS, Schaffitzel C, Saleem MA, 
            Welsh GI, Berger I
  TITLE     Highly efficient CRISPR-mediated large DNA docking and multiplexed 
            prime editing using a single baculovirus.
  JOURNAL   Nucleic Acids Res. 2022 Jul 22;50(13):7783-7799. doi: 
            10.1093/nar/gkac587.
  PUBMED    35801912
REFERENCE   2  (bases 1 to 11337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-07-22- 22"; volume: "50"; issue: 
            "13"; pages: "7783-7799"
COMMENT     Sequence Label: pMMACE DEST CMV PE2
FEATURES             Location/Qualifiers
     source          1..11337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    50..184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(87..106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     141..160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  305..470
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      660..1248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1149..1168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  1383..1606
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1673..2206)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2395..2400,2401..2417,2418..2423))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2494..2527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2569..2693
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2718..2723,2724..2741,2742..2748)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2802..3461
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2868..2887)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3803..4108
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3987..4006
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4149..4273)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        4322..4701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4702..4905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4856..4876
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4947..4966
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4947..4965
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5015..5035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5036..7549,7550..7552,7553..9136)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5036..9136
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11309..11329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catcatcacc atcaccattg agtttaaact gaatgcaatt gttgttgtta acttgtttat
       61 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      181 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
      241 agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa
      301 ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
      361 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
      421 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca accggttgac
      481 ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1321 ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg
     1381 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     1441 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     1501 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     1561 tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca
     1621 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     1681 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     1741 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     1801 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     1861 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     1921 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     1981 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     2041 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     2101 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     2161 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     2221 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2281 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     2341 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     2401 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     2461 cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg
     2521 aagttatctg taactataac ggtcctaagg tagcgagttt aaacatcaac aagtttgtac
     2581 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2641 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     2701 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     2761 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2821 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2881 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2941 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     3001 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     3061 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     3121 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     3181 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     3241 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     3301 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     3361 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     3421 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     3481 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     3541 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     3601 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     3661 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     3721 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3781 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3841 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3901 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     3961 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     4021 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     4081 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     4141 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     4201 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     4261 tgtacaaagt ggttgataag cttaaacact agtatcgatc gcgatgtacg ggccagatat
     4321 agacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4381 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4441 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4501 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4561 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4621 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4681 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4741 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4801 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4861 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     4921 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
     4981 gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacaa
     5041 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
     5101 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     5161 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     5221 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     5281 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     5341 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     5401 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     5461 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     5521 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     5581 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     5641 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     5701 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     5761 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     5821 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     5881 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     5941 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     6001 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     6061 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     6121 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     6181 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     6241 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     6301 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     6361 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     6421 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     6481 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     6541 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     6601 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     6661 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     6721 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     6781 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     6841 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     6901 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     6961 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     7021 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     7081 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     7141 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     7201 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     7261 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     7321 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     7381 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     7441 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     7501 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg ctatcgtgcc
     7561 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     7621 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     7681 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     7741 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     7801 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     7861 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     7921 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     7981 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     8041 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     8101 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     8161 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     8221 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     8281 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     8341 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     8401 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     8461 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     8521 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     8581 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     8641 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
     8701 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     8761 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     8821 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     8881 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     8941 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     9001 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     9061 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     9121 tcagctggga ggtgactctg gaggatctag cggaggatcc tctggcagcg agacaccagg
     9181 aacaagcgag tcagcaacac cagagagcag tggcggcagc agcggcggca gcagcaccct
     9241 aaatatagaa gatgagtatc ggctacatga gacctcaaaa gagccagatg tttctctagg
     9301 gtccacatgg ctgtctgatt ttcctcaggc ctgggcggaa accgggggca tgggactggc
     9361 agttcgccaa gctcctctga tcatacctct gaaagcaacc tctacccccg tgtccataaa
     9421 acaatacccc atgtcacaag aagccagact ggggatcaag ccccacatac agagactgtt
     9481 ggaccaggga atactggtac cctgccagtc cccctggaac acgcccctgc tacccgttaa
     9541 gaaaccaggg actaatgatt ataggcctgt ccaggatctg agagaagtca acaagcgggt
     9601 ggaagacatc caccccaccg tgcccaaccc ttacaacctc ttgagcgggc tcccaccgtc
     9661 ccaccagtgg tacactgtgc ttgatttaaa ggatgccttt ttctgcctga gactccaccc
     9721 caccagtcag cctctcttcg cctttgagtg gagagatcca gagatgggaa tctcaggaca
     9781 attgacctgg accagactcc cacagggttt caaaaacagt cccaccctgt ttaatgaggc
     9841 actgcacaga gacctagcag acttccggat ccagcaccca gacttgatcc tgctacagta
     9901 cgtggatgac ttactgctgg ccgccacttc tgagctagac tgccaacaag gtactcgggc
     9961 cctgttacaa accctaggga acctcgggta tcgggcctcg gccaagaaag cccaaatttg
    10021 ccagaaacag gtcaagtatc tggggtatct tctaaaagag ggtcagagat ggctgactga
    10081 ggccagaaaa gagactgtga tggggcagcc tactccgaag acccctcgac aactaaggga
    10141 gttcctaggg aaggcaggct tctgtcgcct cttcatccct gggtttgcag aaatggcagc
    10201 ccccctgtac cctctcacca aaccggggac tctgtttaat tggggcccag accaacaaaa
    10261 ggcctatcaa gaaatcaagc aagctcttct aactgcccca gccctggggt tgccagattt
    10321 gactaagccc tttgaactct ttgtcgacga gaagcagggc tacgccaaag gtgtcctaac
    10381 gcaaaaactg ggaccttggc gtcggccggt ggcctacctg tccaaaaagc tagacccagt
    10441 agcagctggg tggccccctt gcctacggat ggtagcagcc attgccgtac tgacaaagga
    10501 tgcaggcaag ctaaccatgg gacagccact agtcattctg gccccccatg cagtagaggc
    10561 actagtcaaa caaccccccg accgctggct ttccaacgcc cggatgactc actatcaggc
    10621 cttgcttttg gacacggacc gggtccagtt cggaccggtg gtagccctga acccggctac
    10681 gctgctccca ctgcctgagg aagggctgca acacaactgc cttgatatcc tggccgaagc
    10741 ccacggaacc cgacccgacc taacggacca gccgctccca gacgccgacc acacctggta
    10801 cacggatgga agcagtctct tacaagaggg acagcgtaag gcgggagctg cggtgaccac
    10861 cgagaccgag gtaatctggg ctaaagccct gccagccggg acatccgctc agcgggctga
    10921 actgatagca ctcacccagg ccctaaagat ggcagaaggt aagaagctaa atgtttatac
    10981 tgatagccgt tatgcttttg ctactgccca tatccatgga gaaatataca gaaggcgtgg
    11041 gtggctcaca tcagaaggca aagagatcaa aaataaagac gagatcttgg ccctactaaa
    11101 agccctcttt ctgcccaaaa gacttagcat aatccattgt ccaggacatc aaaagggaca
    11161 cagcgccgag gctagaggca accggatggc tgaccaagcg gcccgaaagg cagccatcac
    11221 agagactcca gacacctcta ccctcctcat agaaaattca tcaccctctg gcggctcaaa
    11281 aagaaccgcc gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggt
//
