LOCUS       sequence-406454-        2485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid containing a terminator sequence from gene 54730 
            from Phaeodactylum tricornutum with EF overhangs used to build a 
            level 1 construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F
  TITLE     Universal loop assembly: open, efficient and cross-kingdom DNA 
            fabrication.
  JOURNAL   Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. 
            Epub 2020 Feb 5.
  PUBMED    32161816
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2020"; volume: "5(1):ysaa001. doi"; pages: " 
            10.1093/synbio/ysaa001. Epub 2020 Feb 5"
COMMENT     Sequence Label: pL0-EF-54730
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1139..1727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1219..1238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1818..2485,1..124))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
       61 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
      121 tcatgcgaaa cgatcctcat cctgtctctt gatcagatat tgatcccctg cgccatcaga
      181 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      241 gcgccccagc tggcaattcc ggttcgcttg ctaagcttgc atgcctgcaa gtcgactcta
      301 gaggagcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
      361 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
      421 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      481 atgggctgcc tgtatcgagt ggtgattttg tgccgagctc attactcgca tccattctca
      541 ggctgtctcg tctcgtctca ggtctcagct ttagaggatc actttcgact gtgaactgaa
      601 aaaaggctgg ttttgttatt tttttcatgc ttgccctggt gtcgttactg cgagaatggt
      661 gcgagaaact tggttcataa gccgtgattg ggctgttctg ccaagtgttt agttgtgctc
      721 gactcgtcac accactgtta gcctcaaagg cgagaaaccc agagagtgta gtatgtagga
      781 aatattcggg atcgatggaa ggaatgtgcc aatgtctctg actgtgagta gaaatttttc
      841 agcgtaacgt attgcaggga atattctctg cgtttgagag gcgacagaag aaataccttt
      901 tgcccaaaga gccgcaaaaa gagtgcgttg cgcgacgacc caacgtacca ttgatacgct
      961 ctgcagacag gaacagagtg tcatcattgc tattgacgct cgagaccacc aggatacata
     1021 aattaccaca actccgagcc cttccaccca cagaatcagg ggataacgca ggaaagaaca
     1081 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1141 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1201 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1261 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1321 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1381 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1441 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1501 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1561 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1621 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1681 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1741 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1801 tgcattctag gtgattatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     1861 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     1921 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     1981 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     2041 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     2101 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     2161 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     2221 tcaatgtcga tcgtggctgg ctcgaagata ccagcaagaa tgtcattgcg ctgccattct
     2281 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     2341 atggtgactt ctacagcgcg gagaatctca ctctctccag gggaagccga agtttccaaa
     2401 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtaac cgtaaccagc
     2461 aaatcaatat cactgtgtgg cttca
//
