LOCUS       sequence-406463-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ABR_DUXA from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ABR_DUXA
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4067..4777,4778..4780,4781..4783))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4109..4130)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4458..4477
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4717..4738
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4780..4789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4865..5068)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4894..4914)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5069..5372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5629..5673)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5858..6091
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6451..6568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7060..7185
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7232..7489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7491..7870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7901..8005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8006..8074,8075..8866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8224..8243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9037..9306,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacc
     1801 aagtgcgcgc cccactgaag tgcgaatctg atgtaaagtt tgtcccattc tgcgtatcct
     1861 cggcgccctg caggccctcc ggaattttgc cctgttcttc ctgctccaag ctagccacgg
     1921 gctcacgctt acgttgaagt aaaaggcgcg aacggcggtt ctggaaccag atctggacac
     1981 gagactcggg gacgccaatc tcctttgcta actcctcgcg gctgtcaatt cctggatagg
     2041 ggtttttcat aaatgctttg atcagtgtgt gaagctgaga tgcggagtaa gtcgtgcgac
     2101 aacgacgagc ttcgcgtgac tgaaattcaa cgcctggctg atcctgcccc tgtgactgtg
     2161 agctttctaa agtctcggct tcgggacgtt tttggaagcc atgacgagca cggcgatttt
     2221 ggaaccagat ctgaatttga ccttttccca taattttgtc aacaatctca ttattgtctt
     2281 tgttaacctt tgtcttatca taacactttt cgtaacacaa aatacgtaac gactgtgagc
     2341 cctccaattc aatttcaaac tcttcgtccc acttaggctc cgctgtgtcg cggaaaacac
     2401 gtgtcttagc cttgctcaca aagtacccaa aactgtccac ttctaaggtg cagtataaat
     2461 tagcggactg tttaaatccc ttagcggagt gaacgattac gtgcaagaac ccatacagtc
     2521 ccggagattc atcatcatct ttattgcttg tgactggaat gttatgaaca gtacgtaatt
     2581 tgaagcaact gcccgtaagg acctgaagtt ccacagacga aagtacaaag gcctgcagat
     2641 cctttttctg taacttttgg attgcctcgc gccactcgct gcgttcatag tcagatgata
     2701 ataaaaacag atacgacttg ccgttacggt tgtggatacg aaaaggaatc gtaggagaat
     2761 ttaacagtaa aagaaattcg ttttcaaaca tttttttttt caaacgttcg atagcacgag
     2821 attggccctt gttcgccttt tctttttgga tctcagactt caacgccgaa atcttcattt
     2881 tcatgtcttc taattcatgg tcggggaacg ggtgcacctg tggtgaggct tcactctcct
     2941 caggactcgg aaataccaga tcagccaatg gaatgtacca cttgcaatca tattgttggt
     3001 gcttccccgc agaggtcttc ttcagctttg cacacaataa cacgtcagtg aacaaaaata
     3061 cgtgacgcaa cttacggcta ctttcagaca cttccaccag gaacccatct tttaccagct
     3121 ggcgggtctc acccttcgga gtagttaccg cggtacggcg ggggtcgata tcttcattaa
     3181 tactgctcag aaaattctga ctgatacgaa gtgcgtcctg taacaatgga tagtccgggt
     3241 gatccaccgg tgtatgtttc aaaaggtcgt gtaatacaag cgttgaacga gttacgcggt
     3301 cgatgggctt gtaaagcagt gcctccatcg tcacactggt gtgagaatcc ttactatctt
     3361 taggaccctt aacctttaat tcctcgctaa tcttttgaaa ctggttgttc gattgtgagc
     3421 atttctccgc agtctccagg gctactttgt agttatcaac gaacgcttta tacaccccta
     3481 actgggatgc aagtttctgg aataagtggc ccattgtcac ttgggaatcc cattgttgga
     3541 cctttggaca caagttgtcg taaaactcct tatggatctc gtagatatcc tggattttat
     3601 agaaaatcgt ttcaatctgc tgaattgtaa gtaccggctg acttgtggtc gctgtggctt
     3661 tcaacggctt catcggaagc agtaaagcct ccagctggtt gatatagatc tcttcactgg
     3721 ccagaaagcc cgacagaaca agtttgcgca tttctaagcc tttgcctgcc tccacgccgg
     3781 gtgctaaccc ttccggcggc gtgggtgata ccccatcacc acctccttga gagcgggccg
     3841 aaagttgggg actcatcgta ggtgactcgt caatgtaagg cattgtctcc gagccttcag
     3901 gaggcccttt ttgttcctca tttccctctc catcatactc atctgtaccg tagctaaaat
     3961 ttgagtacaa tgtgtcaatc cagctcaggc gaggcagacc gcggtgagac agtggctcca
     4021 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     4081 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     4141 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     4201 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     4261 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     4321 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     4381 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     4441 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     4501 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     4561 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     4621 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     4681 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     4741 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     4801 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     4861 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     4921 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     4981 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     5041 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     5101 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     5161 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5221 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5281 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5341 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5401 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     5461 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     5521 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     5581 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     5641 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     5701 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     5761 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     5821 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     5881 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     5941 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     6001 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     6061 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     6121 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     6181 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     6241 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     6301 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     6361 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     6421 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     6481 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     6541 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
     6601 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
     6661 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
     6721 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     6781 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     6841 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     6901 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
     6961 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     7021 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     7081 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     7141 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     7201 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     7261 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     7321 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     7381 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     7441 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
     7501 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     7561 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     7621 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     7681 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     7741 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     7801 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     7861 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9301 taccgg
//
