LOCUS       sequence-406464-        8358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ADIPOR2_FOXJ2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ADIPOR2_FOXJ2
FEATURES             Location/Qualifiers
     source          1..8358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3119..3829,3830..3832,3833..3835))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3161..3182)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3510..3529
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3769..3790
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3832..3841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3917..4120)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3946..3966)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4121..4424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4681..4725)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4910..5143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5503..5620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6112..6237
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6284..6541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6543..6922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6953..7057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7058..7126,7127..7918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7276..7295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8089..8358,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 tgatcaggtc ccaatcaaag tcatcgggaa tttcctcatt ggctccagga ggcggaacgc
     1861 tggggcgagg gaccatgtgg tgtgcagggc ggttgtaacc ggctgaatcc tgggtgggaa
     1921 tgggatacat gggtgatggt cccggataca agtgaggtgc ttggggatgt ccgtatgaat
     1981 taacttgact catagcactc tcttgctttc ccgatgttaa agcacaagaa tccgcgatac
     2041 gtgcaggcgc gggagggcga tgggtgccgc cctgaggggg cacatgtccg gtttgtgtca
     2101 gaaaatgatt cagagagtcg cacaaattag caatgtgatg ctggtccaag gtccaattct
     2161 tttgctcagc ctgacgaagg gactccatta actcactgaa ctgggattgt tcaatactgc
     2221 tccaattcag acggttgacc atcttgaagg attccttcag agaatcaata ttactgcacc
     2281 aatccgatgg gaatgcaaaa ccagtgttat tgataggggc ttgaccctgg gcttggggtt
     2341 gcgggggcgg cggctgctga gcagggtggc tgtgtgggtg gtgttgatgc ggatgatagc
     2401 ctccatgttg aagcggaggg ggatgcatcg ccataacagg gggcgggcca tatccttccg
     2461 cacccgcttg cttccctcct ggcggtgtac aaccgtcggt gctgggggta tgtaatggag
     2521 gggcacctcc gacagctgaa gggccttgag ctggggcctg ttgctgctga ggttgtgatt
     2581 gttgaggtgg tggttgaggg ggttgctgct gctgctgttg ctgttgtggt ggcggtggct
     2641 gctgctgctg gtacatataa tagttattgg acggcggaat gtctcccagc aggctggaga
     2701 atccgtgctg tgatgaagaa gatgactttt ctaacatgga cttatataag ttacggaagg
     2761 accaactcaa gtcctggaaa ttgatctctg agtagctgaa cttgaagtcg tgattggtat
     2821 tatacagtgg tggaggtccc tcggccgatt cgcgtccgct tgcgcctgcg gccacggctc
     2881 ctccgtccac gcttcctgtg ccctttttat ggtgggacga aaggacgctc gcttccaaga
     2941 tcggctccaa atcaccctgg tgagaatcgt tatcaccacg gcgtgttcca tccaattgat
     3001 gacccttacg caaacggatg tcgggttcag gagtacgact gcaccccaaa cgattctcgg
     3061 tgggctcgtt cattccccgg gaagcggccg cacttccccc ggagcctccg cttccgccct
     3121 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     3181 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     3241 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     3301 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     3361 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     3421 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     3481 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     3541 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     3601 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     3661 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     3721 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     3781 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     3841 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcggttc actaaacgag
     3901 cccggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     3961 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     4021 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     4081 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     4141 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     4201 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     4261 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     4321 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     4381 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     4441 ggctatgaac taatgacccc gtaattgatt actattaata actagcacgc gttcgaagct
     4501 tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     4561 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     4621 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     4681 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     4741 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     4801 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     4861 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     4921 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     4981 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     5041 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     5101 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     5161 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     5221 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctccttatgg
     5281 tgttttacta aacttttcca tgttctaatc ctcatcctgt ctacttgcca cacaatcatc
     5341 acctgccatc tgttttccat aatccctaat gatctttgct tttcttcttg gcactacttt
     5401 tatgtcacta ttatcttgta ttactactgc cccttcacct ttccagagga gctttgctgg
     5461 tcctttccaa agtggatttc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     5521 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     5581 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     5641 ttgtactgct gtcttaagat gttcagcctg atctcttacc tgtcctataa ttttctttaa
     5701 ttctttattc atagattcta ctactccttg actttgggga ttgtagggaa tttccaggtc
     5761 tgaagatcct gctgcttgct gtgctttttt cttacttttg ttttgctctt cctctatctt
     5821 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     5881 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     5941 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccagtattaa ctgcgaatcg
     6001 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     6061 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     6121 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     6181 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     6241 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     6301 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     6361 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     6421 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     6481 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     6541 cccatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     6601 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     6661 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     6721 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     6781 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     6841 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     6901 taactagtca ataatcaatg tcgacccagg tggcactttt cggggaaatg tgcgcggaac
     6961 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7021 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7081 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7141 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7201 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7261 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7321 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7381 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7441 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7501 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7561 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7621 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7681 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7741 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7801 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7861 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7921 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7981 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8341 taagtcgtgt cttaccgg
//
