LOCUS       sequence-406468-        2124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent 
            corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_CRF0.5
FEATURES             Location/Qualifiers
     source          1..2124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1248..1269
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1375..1396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1636..1655)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtatgg gagggcaccc gcagctccgt
      121 ctcgtcaagg cccttctcct tctggggctg aaccccgtct ctgcctccct ccaggaccag
      181 cactgcgaga gcctgtccct ggccagcaac atctcaggac tgcagtgcaa cgcatccgtg
      241 gacctcattg gcacctgctg gccccgcagc cctgcggggc agctagtggt tcggccctgc
      301 cctgcctttt tctatggtgt ccgctacaat accacaaaca atggctaccg ggagtgcctg
      361 gccaatggca gctgggccgc ccgcgtgaat tactccgagt gccaggagat cctcaatgag
      421 gagaaaaaaa gcaaggtgca ctaccatgtc gcagtcatca tcaactacct gggccactgt
      481 atctccctgg tggccctcct ggtggccttt gtcctctttc tgcggctcag gagcatccgg
      541 tgcctgcgaa acatcatcca ctggaacctc atctccgcct tcatcctgcg caacgccacc
      601 tggttcgtgg tccagctaac catgagcccc gaggtccacc agagcaacgt gggctggtgc
      661 aggttggtga cagccgccta caactacttc catgtgacca acttcttctg gatgttcggc
      721 gagggctgct acctgcacac agccatcgtg ctcacctact ccactgaccg gctgcgcaaa
      781 tggatgttca tctgcattgg ctggggtgtg cccttcccca tcattgtggc ctgggccatt
      841 gggaagctgt actacgacaa tgagaagtgc tggtttggca aaaggcctgg ggtgtacacc
      901 gactacatct accagggccc catgatcctg gtcctgctga tcaatttcat cttccttttc
      961 aacatcgtcc gcatcctcat gaccaagctc cgggcatcca ccccgcgcga caccgacgcg
     1021 ctggacctgg aggagggagg aaacgtctat atcaaggccg acaagcagaa gaacggcatc
     1081 aaggcgaact tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac
     1141 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1201 agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1261 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1321 ggagggagca tggtgagaaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1381 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat
     1441 gccaccgagg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1501 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1561 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc
     1621 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1681 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1741 ctggggcaca agctggagta caacaccgga gcagcagcac gctggcgcgg gcggcagtac
     1801 aggaaggctg tgaaagccac tctggtgctg ctgcccctcc tgggcatcac ctacatgctg
     1861 ttcttcgtca atcccgggga ggatgaggtc tcccgggtcg tcttcatcta cttcaactcc
     1921 ttcctggaat ccttccaggg cttctttgtg tctgtgttct actgtttcct caatagtgag
     1981 gtccgttctg ccatccggaa gaggtggcac cggtggcagg acaagcactc gatccgtgcc
     2041 cgagtggccc gtgccatgtc catccccacc tccccaaccc gtgtcagctt tcacagcatc
     2101 aagcagtcca cagcagtctt gtaa
//
