LOCUS       sequence-406498-        2106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent urocortin (UCN) sensor
            GRAB_UCN1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_UCN1.0
FEATURES             Location/Qualifiers
     source          1..2106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1233..1254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1360..1381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1621..1640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtgacg cggcgctgct cctcagcctg
      121 ctggaggcca actgcagcct ggcgctggcc gaagagctgc tcctggacgg ctggggagtg
      181 cccccggacc ccgaaggtcc ctacacctac tgcaacacga ccttggacca gatcgggacc
      241 tgctggccac agagcgcacc cggagcccta gtagagagac cgtgccccga gtacttcaat
      301 ggcatcaagt acaacacgac ccggaatgcc tacagagagt gcctggagaa cgggacctgg
      361 gcctcaaggg tcaactactc acactgcgaa cccattttgg atgacaagca gagaaagtat
      421 gacctgcatt accgaatcgc cctcattgtc aactacctgg gtcactgtgt ttccgtggtg
      481 gccctggtgg ccgctttcct gcttttccta gtgctgcgga gtatccgctg cctgaggaat
      541 gtgatccact ggaacctcat caccaccttc attctgagaa acatcgcgtg gttcctgctg
      601 caactcatcg accacgaagt gcacgagggc aatgaggtct ggtgccgctg catcaccacc
      661 atcttcaact attttgtggt caccaacttc ttctggatgt ttgtggaggg ctgctacctg
      721 cacacggcca ttgtcatgac gtactccaca gagcacctgc gcaagtggct tttcctcttc
      781 attggatggt gcattccctg ccctatcatc atcgcctggg cagttggcaa actctactat
      841 gagaatgagc agtgctggtt tggcaaggaa gctggtgatt tggtggacta catctaccag
      901 ggccccgtca tgcttgtgct gttgatcaat tttgtatttc tgtttaacat cgtcaggatc
      961 ctgatgacga agttacgagc atacaccggc cccgacaccg acgcgctgga cctggaggag
     1021 ggaggaaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcacc
     1081 atccgccaca actgcgagga cggcggcgtg cagctcgcca cccactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa
     1201 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
     1321 agaaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1381 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac cgagggcaag
     1441 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1501 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1561 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     1621 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1681 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1741 gagtacaaca ccggagcagc agcacgctgg cgcgggaggc agatgaggaa ggcagtgaag
     1801 gccacgctgg tcctcctccc cctgttgggc atcacctaca tgctcttctt tgtcaatcct
     1861 ggcgaggacg acctgtccca gattgtgttc atctacttca actctttcct gcagtccttc
     1921 cagggtttct ttgtgtccgt tttctactgc ttcttcaatg gagaggtgcg cgcggccctg
     1981 agaaagcggt ggcaccgctg gcaggaccac cacgccctcc gggtgcctgt ggcccgggcc
     2041 atgtccatcc ctacgtcgcc caccaggatc agcttccaca gcatcaagca gacagctgct
     2101 gtgtga
//
