LOCUS       sequence-406510-        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent cholecystokinin (CCK) 
            sensor GRAB_CCK1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_CCK1.0
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1221..1242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1348..1369)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1609..1628)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtgagc tgctaaagct gaaccggagc
      121 gtgcagggaa ccggacccgg gccgggggct tccctgtgcc gcccgggggc gcctctcctc
      181 aacagcagca gtgtgggcaa cctcagctgc gagccccctc gcattcgcgg agccgggaca
      241 cgagaattgg agctggccat tagaatcact ctttacgcag tgatcttcct gatgagcgtt
      301 ggaggaaata tgctcatcat cgtggtcctg ggactgagcc gccgcctgag gactgtcacc
      361 aatgccttcc tcctctcact ggcagtcagc gacctcctgc tggctgtggc ttgcatgccc
      421 ttcaccctcc tgcccaatgt catgggcaca tggatctttg gcaccgtcat ctgcaaggcg
      481 gtttcctacc tcatgggggt gtctgtgagt gtgtctacgc taagcctcgt ggccatcgca
      541 ctggagcggt acagcgccat ctgccgacca ggccaggcac gagtgtggca gacgcgctcc
      601 cacgcggctc gcgtgattgt agccacgtgg ctgctgtccg gactactcat ggtgccctac
      661 cccgtgtaca ctgtcgtgca accagtgggg cctcgtgtgc tgcagtgcgt gcatcgctgg
      721 cccagtgcgc gggtccgcca gacctggtcc gtactgctgc ttctgctctt gttcttcatc
      781 ccgggtgtgg ttatggccgt ggcctacggg cttatctctc gcgagctcta cttagggctt
      841 cgccttccgc cggggggcac cgagcgcagg cccaacggtc tgggccccga gcgcagcgcg
      901 ggcccggggg gcgcagaggc cgaaccgctg cccacccagc tcaacggcgc ccctggcgag
      961 cccgcgccgg ccgggccgcg cgacaccgac gcgctggacc tggaggaggg aggaaacgtc
     1021 tatatcaagg ccgacaagca gaagaacggc atcaaggcga ctttcgtgat ccgccacaac
     1081 atcgaggacg gcggcgtgca gctcgccagc cactaccagc agaacacccc catcggcgac
     1141 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac
     1201 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1261 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag aaagggcgag
     1321 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1381 aagttcagcg tgcacggcga gggtgagggc gatgccacct tgggcaagct gaccctgaag
     1441 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1501 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1561 tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac
     1621 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1681 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacacc
     1741 ggagcaggcg caaagctgct ggctaagaag cgcgtggtgc gaatgttgct ggtgatcgtt
     1801 gtgctttttt ttctgtgttg gttgccagtt tatagtgcca acacgtggcg cgcctttgat
     1861 ggcccgggtg cacaccgagc actctcgggt gctcctatct ccttcattca cttgctgagc
     1921 tacgcctcgg cctgtgtcaa ccccctggtc tactgcttca tgcaccgtcg ctttcgccag
     1981 gcctgcctgg aaacttgcgc tcgctgctgc ccccggcctc cacgagctcg ccccagggct
     2041 cttcccgatg aggaccctcc cactccctcc attgcttcgc tgtccaggct tagctacacc
     2101 accatcagca cactgggccc tggctga
//
