LOCUS       sequence-406517-        2178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) 
            sensor GRAB_NPY1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_NPY1.0
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1239..1260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1366..1387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1627..1646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtaatt caacattatt ttcccaggtt
      121 gaaaatcatt cagtccactc taatttctca gagaagaatg cccagcttct ggcttttgaa
      181 aatgatgatt gtcatctgcc cttggccatg atatttacct tagctcttgc ttatggagct
      241 gtgatcattc ttggtgtctc tggaaacctg gccttgatca taatcatctt gaaacaaaag
      301 gagatgagaa atgttaccaa catcctgatt gtgaaccttt ccttctcaga cttgcttgtt
      361 gccatcacgt gtctcccctt tacatttgtc tacacattaa tggaccactg ggtcttcggt
      421 gaggcgatgt gtaagttgaa tccttttgtg caatgtgttt caatcactgt gtccattttc
      481 tctctggttc tcattgctgt ggaacgacat cagctgataa tcaaccctcg agggtggaga
      541 ccaaataata gacatgctta tgtaggtatt gctgtgattt gggtccttgc tgtggcttct
      601 tctttgcctt tcctgatcta ccaagtaatg actgatgagc cgttccaaaa tgtaacactt
      661 gatgcgtaca aagacaaata cgtgtgcttt gatcaatttc catcggactc tcataggttg
      721 tcttatacca ctctcctctt ggtgctgcag tattttggtc cactttgttt tatatttatt
      781 tgctacttca agatatatat acgcctaaaa aggagaaaca acatgatgcc acccagccgc
      841 cggggtccgg acgccgtcgc cgcgccgccg gggggcaccg agcgcaggcc caacggtctg
      901 ggccccgagc gcagcgcggg cccggggggc gcagaggccg aaccgctgcc cacccagctc
      961 aacggcgccc ctggcgagcc cgcgccggcc gggccgcgcg acaccgacgc gctggacctg
     1021 gaggagggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     1081 ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     1201 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     1321 atggtgagaa agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1381 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccaccgag
     1441 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1501 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1561 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     1621 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1681 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1741 aagctggagt acaacaccgg agcagcagca cgctggcgcg ggcggcagaa cgaaaccaaa
     1801 agaatcaata tcatgctgct ctccattgtg gtagcatttg cagtctgctg gctccctctt
     1861 accatcttta acactgtgtt tgattggaat catcagatca ttgctacctg caaccacaat
     1921 ctgttattcc tgctctgcca cctcacagca atgatgtcca cttgtgtcaa ccccatattt
     1981 tatgggttcc tgaacaaaaa cttccagaga gacttgcagt tcttcttcaa cttttgtgat
     2041 ttccggtctc gggatgatga ttatgaaaca atagccatgt ccacgatgca cacagatgtt
     2101 tccaaaactt ctttgaagca agcaagccca gtcgcattta aaaaaatcaa caacaatgat
     2161 gataatgaaa aaatctaa
//
