LOCUS       sequence-406553-        1992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2h and a membrane-localized mcherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Feng J, Dong H, Lischinsky JE, Zhou J, Deng F, Zhuang C, Miao X, 
            Wang H, Li G, Cai R, Xie H, Cui G, Lin D, Li Y
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRAB(NE) sensors.
  JOURNAL   Neuron. 2024 Jun 19;112(12):1930-1942.e6. doi: 
            10.1016/j.neuron.2024.03.001. Epub 2024 Mar 27.
  PUBMED    38547869
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2024.03.001"; journalName: "Neuron"; date: 
            "2024-06-19- 19"; volume: "112"; issue: "12"; pages: "1930-1942.e6"
COMMENT     Sequence Label: pDisplay-NE2h-IRES-mCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1185..1206
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1312..1333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1573..1592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc ggctccctgc agccggacgc gggcaacgcg
      121 agctggaacg ggaccgaggc gccggggggc ggcgcccggg ccacccctta ctccctgcag
      181 gtgacgctga cgctggtgtg cctggccggc ctgctcatgc tgctcaccgt gttcggcaac
      241 gtgctcgtca tcatcgccgt gttcacgagc cgcgcgctca aggcgcccca aaacctcttc
      301 ctggtgtctc tggcctcggc cgacatcctg gtggccacgc tcgtcatccc tttctcgctg
      361 gccaacgagg tcatgggcta ctggtacttc ggcaaggctt ggtgcgagat ctacctggcg
      421 ctcgacgtgc tcttctgcac gtcgtccatc gtgcacctgt gcgccatcag cctggaccgc
      481 tactggtcca tcacacaggc caaagagtac aacctgaagc gcacgccgcg ccgcatcaag
      541 gccatcatca tcaccgtgtg ggtcatctcg gccgtcatct ccttcccgcc gctcatctcc
      601 atcgagaaga agggcggcgg cggcggcccg cagccggccg agccgcgctg cgagatcaac
      661 gaccagaagt ggtacgtcat ctcgtcgtgc atcggctcct tcttcgctcc ctgcctcatc
      721 atgatcctgg tctacgtgcg catctaccag atcgccaagc gtcgcacccg cgtgccaccc
      781 agccgccggg gtccggacgc cgtcgccgcg ccgccggggg gcaccgagcg caggcccaac
      841 ggtctgggcc ccgagcgcag cgcgggcccg gggggcgcag aggccgaacc gctgcccacc
      901 cagctcaacg gcgcccctgg cgagcccgcg ccggccgggc cgcgcgacac cgacgcgctg
      961 gacctggagg agggaggaaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag
     1021 gcgaacttcc acatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac
     1081 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1141 gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1201 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga
     1261 gggagcatgg tgagaaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1321 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
     1381 accgagggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1441 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1501 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
     1561 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1621 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1681 gggcacaagc tggagtacaa caccggagca gcagcacgct ggcgcgggcg gcagaaccgc
     1741 gagaagcgct tcattttcgt gctggccgtg gtcatcggag tgttcgtggt gtgctggttc
     1801 cccttcttct tcacctacac gctcacggcc gtcgggtgct ccgtgccacg cacgctcttc
     1861 aaattcttct tctggttcgg ctactgcaac agctcgttga acccggtcat ctacaccatc
     1921 ttcaaccacg atttccgccg cgccttcaag aagatcctct gtcgggggga caggaagcgg
     1981 atcgtgttgt aa
//
