LOCUS       sequence-406575-        9372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ARID1B_ZNF384 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ARID1B_ZNF384
FEATURES             Location/Qualifiers
     source          1..9372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4133..4843,4844..4846,4847..4849))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4175..4196)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4524..4543
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4783..4804
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4846..4855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4931..5134)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4960..4980)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5135..5438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5695..5739)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5924..6157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6517..6634
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7126..7251
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7298..7555
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7557..7936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7967..8071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8072..8140,8141..8932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8290..8309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9103..9372,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 agctagccag gtgttcaacc tggatggtgc ttgtggtcac ggtcagacag atgtctttat
     1861 gatgttcggc agttttgtac ggagtcagat cgaagctaca ttgaggcggt gggttcggat
     1921 tgctgtcgcc gccaccaccg ccctgtggag cggcgcccgg agactggaag tgcggcggcg
     1981 gttgctgctg ctgctgctgt tgttgctgtt gctgctgctg ttgttgttgg ctcgcctggc
     2041 tcgcctgcgc ttgagcttga gcctgggcct gcgcctgcgc ctgcgcctgc gcttgcgcct
     2101 gcgcctgtgc tacagcagcc gccgccgccg cagcttggac ttgttgttgc aggtctggcg
     2161 ggttgtgctt acgcatgtgt ttcatcagat atgtctcgct ggtatatgcg cgtgagcaga
     2221 tagtgcacgt gtacacctta gcatgcttca ccgtatgggt gctaagatgg acctctaagc
     2281 tggctgcatc ggtatacgcg cggtgacaat tatggcattt aaacggtttg tccttgttgt
     2341 gctgacgacg gtggctctgc aggttggaca gttgggtgaa tgccttctcg cagcccggat
     2401 gtgcgcactt atacggacga tcaccggtat ggatacgcgt gtgttgctgc aggtgtgaca
     2461 gctggcggaa gcttttttcg caaaaattac agctgtaggg tttcgcgccg gagtggatac
     2521 gaatgtgctg cgcgagatat gaggagtttg cgaaggtttt cgagcagtgc gggcacttgt
     2581 gcggtttcgt ttccgtgtgg gacttggaat gaatctgcat ctcggacttt gaataaaaag
     2641 tcaagctgca catacggcag cggtacgttt tgccgtcttt ctgatggtcg tcgtcatctt
     2701 ccgggctcag gacgtacgga tcattcatct caggcaaacc gctttccagc atacgcttct
     2761 ttttgcgtcc acgcggtggt ttcggggcca cgctgccacc accaccgccg ccgccaccgc
     2821 cctcctcagt caaggtgctc gcaactttct tactcagatc ggggactacc tgcagcgcct
     2881 ggctgccagg cggcagcgca gaaacaatca ttggcgcgga gatcgggaag gtttgcgcag
     2941 acgatgcggt ggtcacgagg gagccgctcg ggctggtgat aaccaaaccc ggacctctgc
     3001 tctgactacc ttcgcgacgc caacgttggc tgcagctaac acccgccgtc atcaagccgg
     3061 tagacggcac gggaaccacg gtaatgttct gggtcacgga cgcctgactg tgcggggtca
     3121 gttgatcact cttgctctcg gtatccatag agatacctga cggcaagctc acggaagccg
     3181 gcaccgtaag taaggtcggg taatgtggcg gagccaaacc gcaacccttc tccggcagca
     3241 gctggtcttt catcttgtta ataaacatgg tattttcgat ttgaccacta acggtcggaa
     3301 tgctcggcca gaagtacggg ttcgaattaa agtggctctc ttccatccga ccggcggagc
     3361 tattaaccat tgccggcgcc atgctgtacg gcggtgcctg ggtgttcatc agagagctgg
     3421 agttgttcat cggctggctg tacgggctag atgatgccat caggccagac tggttcatgc
     3481 ccgggaagga gccttgacgg ctttgcgcgc tgttcgcagc agcctgcata accgctgccg
     3541 ccgcctcttg ggccttgcgg ttcacggtcg gcatcggcgg acccatgcca gggccacctt
     3601 gttggctcat tcccggggaa gacggcgtca tgctagacat attacccgga aacggacggt
     3661 tttgcatacc tgccgacggc atacgaccca gtggtaccgc accacacggc tggctcgggc
     3721 cttggccatg catctggtta ttcgcgctaa tccccatacc tggacccgga ccggagtagc
     3781 tcgcagacgg aacgccgcta tacgctggcg ggcgggaata gttgccttgc ggaccgtatt
     3841 ggctcatctg cgggccgtag gtaccggagc tgttgctctg ttggaacgaa ctgatgccag
     3901 cgtgcatctg gccacccgga gaagggtgcg ggctcatgct cgggccggtc tgttgcggac
     3961 catactgtgc catctgcgga ttacgctgcg tgccagccat aaaaccacgt tcctgaggca
     4021 tcggcgattg acttaacgcg ggatggctag aggactctga ttgacttccg gggggctgtg
     4081 gaggcattcc ccgggaagcg gccgcacttc ccccggagcc tccgcttccg cccttgtaca
     4141 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     4201 cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag tggttgtcgg
     4261 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4321 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     4381 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4441 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4501 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4561 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4621 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     4681 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     4741 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     4801 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     4861 gtggatcccg ggcccgcggt accgtcgact gcagaattcg gttcactaaa cgagcccggt
     4921 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     4981 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     5041 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     5101 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     5161 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     5221 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     5281 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     5341 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     5401 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     5461 gaactaatga ccccgtaatt gattactatt aataactagc acgcgttcga agcttgagct
     5521 cggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     5581 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     5641 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     5701 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     5761 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     5821 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     5881 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     5941 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     6001 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     6061 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     6121 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     6181 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     6241 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctt atggtgtttt
     6301 actaaacttt tccatgttct aatcctcatc ctgtctactt gccacacaat catcacctgc
     6361 catctgtttt ccataatccc taatgatctt tgcttttctt cttggcacta cttttatgtc
     6421 actattatct tgtattacta ctgccccttc acctttccag aggagctttg ctggtccttt
     6481 ccaaagtgga tttctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     6541 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     6601 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtac
     6661 tgctgtctta agatgttcag cctgatctct tacctgtcct ataattttct ttaattcttt
     6721 attcatagat tctactactc cttgactttg gggattgtag ggaatttcca ggtctgaaga
     6781 tcctgctgct tgctgtgctt ttttcttact tttgttttgc tcttcctcta tcttgtctaa
     6841 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     6901 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     6961 tatttgtcta cagccttctg atgtttctaa caggccagta ttaactgcga atcgttctag
     7021 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     7081 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     7141 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     7201 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     7261 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     7321 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     7381 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     7441 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc
     7501 ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctcccatg
     7561 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     7621 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     7681 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     7741 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     7801 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     7861 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     7921 gtcaataatc aatgtcgacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8041 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8101 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8161 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8221 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8281 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8341 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8401 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8461 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8521 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8581 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8641 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8701 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8761 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8821 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8881 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8941 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9001 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9361 gtgtcttacc gg
//
