LOCUS       sequence-406581-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ATF7IP_JAK2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ATF7IP_JAK2
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(6389..7099,7100..7102,7103..7105))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6431..6452)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6780..6799
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7039..7060
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7102..7111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7187..7390)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7216..7236)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7391..7770
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7801..7905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7906..7974,7975..8766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8124..8143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8937..9206,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatc
     1801 cagccatgtt atcccttatt tgatccactc gaagagctag atccctaaag gaggggcgtt
     1861 gatttacatt attgttccag cattctgtca tgatcatata gatctcatct gggcatccat
     1921 ctggtcttgg taatcttcca ttattcttca aaagttctat caaatggaac acgatcatct
     1981 gtccttgttt gtcattgcca atcatacgca taaattccgc tggtggactt ttactcttct
     2041 caatgtatgt gaaaagttca tacagaacca ctccaaagct ccaaacatct gaggccacag
     2101 aaaacttgct ctctgtcagt gattctggag cataccagaa tatgggactt tcaccaggtt
     2161 cttttacttt atagtattct ttgtcttgtg gcaagacttt ggttaaccca aaatctccaa
     2221 ttttaactct gttctcgttc tccaccaata tatttctcgt tgccagatcc ctatggatat
     2281 acctttttgt accaagatac tccataccct tgcatatctg agatgtgtac tgcagaagtt
     2341 ttatgtgatc tatccgttct ttatgttttt gaagatagtc tcgtaaactt ccatatggta
     2401 aatattccat aattaatttt agattacgcc gaccagcact gtagcacact cccttgtact
     2461 ttacgatgtt gtcatgctgt agggatttca ggatttcaat ttccctttca aagtctctta
     2521 ggtgctcttc agtactatgc tgaagctttt ttacagcgac cacctcccca gtgttgtcct
     2581 gtagagggtc ataccggcac atctccacac tcccaaaatt acccttgcca agttgctgta
     2641 gaaatttcaa atgtctctct tcaaactgtg taggatcccg gtcttcaaag gcaccagaaa
     2701 accccagggc acctatcctc atatttggta acatgtcatt ttctgttaat agttcataat
     2761 ctggagtaaa caaactgtta agatctcgta tgatggctct gaaagaaggc ctgaaatctg
     2821 gttcataatc catacaatta tttataaggt ttgctaattc tgcccacttt ggtgcaggaa
     2881 gctgatgcct atcttcataa aattgtagct ttctttgaga atccagagca cttagaggtt
     2941 tatctcctcc actgcagatt tcccacaaag tggtaccaaa actccatttg tctgttgcca
     3001 aatttaaatt tttaggattt tcaatgcatt caggtggtac ccatggtatt ctctcctgaa
     3061 gaatattttg gggattgacc tgccgaacag caggagcctg cataacagtt cctcgcaagg
     3121 gagcctgagc tggtggtgca ggaagagttg tataaaccac ctggtggttt gcaggagctc
     3181 ttggtacagg gagtcttgtg gtcacctgag ttactggacg atgtgttaca ttcacggtag
     3241 ttggagctgt aggtagtaag tgtatggtgg tctgagatgg tccacttgtt ggcaccccag
     3301 atggttgaag aggcggtgct ggttgtatgg gttgcaatgg tcgtgacaca ggctgagaag
     3361 aactcatggt tgatacagga gtgtgattta gttttttggg gtcttgtgaa gctccactct
     3421 cttcatcatc cattgtgaga tcaatgacac cacttgaatc actgccagtg gcttttccac
     3481 actgtgatgt gctatcagct gctttcttac tgacagaagc atctattgtt ttgtttgtct
     3541 ggttttcaat tcttggggtg gtattgctgt tctgttctgc agcagacgaa gtactaaatg
     3601 cagaaattgg aattttcatc tgtataggac ctcgattagt tgatggacta tagagtcctg
     3661 atgggcccac tgtgggtaaa gaagtccttg tggcttgtac aatagagtgt gctgttggaa
     3721 cagcctgcac agcaagtgtt gttcccaatg gtgcagcact ggcagtaggg ttcctttgaa
     3781 tactaggact gggcacagaa ggaacgttgt ttggtttagt gggatttggc aaggatggca
     3841 aggatactgg atttttggta agaccactca ctgtaggtgg gctttgcaca gaaatgaatt
     3901 caacattgcc agatggttga ttagtcacca aagtccctgg atggctctgt agaagctgag
     3961 gctgggagga tactgcaaca ggtacatgca aaatcactgg caaaggctgt aaagagattg
     4021 taggctgggg atttccacta ggcacctgag tagtagcaac tacagtagct gtattgggtg
     4081 caggaagaac tgacgttgct gtgagggaac ccgaagtcac tggagtctgc aatttaggtt
     4141 gactactgac aactgctgga ggagtgacaa gattggttga agatactgta ttcacaggag
     4201 tttgaaagga tggtggtgca ctctcgctta catttctttt ggactccagc atctgtctca
     4261 ctgtgcctgc atttctgtaa gacatgttat tattgctgtt gacatcattt acagtttttc
     4321 ctggtgatac tggtgggttg ggtggatgtt catgtctttt cttaagatct tctttggctg
     4381 cttcaaagcg tttggttaac ctggctatct tggcctgtag ttcagtgaga actgttttat
     4441 gcctcttgtt acattcaatc ttttccactc gtgttttcaa ttctgccaaa gtcttatcaa
     4501 atacagcaca ctgcagcgca cacaattttt cttccagcaa ccactgtata accttttgaa
     4561 gtttctggtt aatgtctcct ttggctgtaa tctttacttg aaattctgcc tcatattctt
     4621 cttccatata tcgacgacgt ttagactgta cattgtccat gtcttctgat ttagaacgtt
     4681 ttcgtctaga aaattcattt ttctcagaag gtctttcatc atcttcatgt attacttgct
     4741 cttcttcctc aggtctgttg tccttttcat tactttctac ttctgctgga gagcacgttt
     4801 cattttgcga tataacttca gactcatctt tttcacccga aatatcctct tcatcagaga
     4861 ggaccagaaa ggcttctttt ggcaaactct cactactttc ttgtttagat ggtgaaccaa
     4921 aagaactttc cattttctct tccaaatctg gatttgtctc atcgattgga aggagagatg
     4981 ttttagaaaa gcaagtaagt tcatcctcag aaggtgcaag cttttccaaa ataggaatga
     5041 tttcatctgt ctccatagag tctgtatttt ctaagatatt ctcacttttg ttatcttcaa
     5101 taacattttc attaatcgtt tctacaagat ctgcagaagt ttcatcttca ttcttttcac
     5161 cttggtcaat ttccatactg ctagatatag catcttctcc aagagcaagc tctgtgataa
     5221 tatcttcctc taccttcttt tcattttcag gaggtcgatc tgaacaaata gttgtatcat
     5281 ctgtttctag agtttcttca ttagcatcaa tattattatc agctttattt ttctcatcca
     5341 atgagtcttt actctgaatt tgctctaatt tttcatcagc tccatttttt tcaagaaaat
     5401 catcatcttt attgctactt ggttctatat tgtcaatttc aggaactggt tcacaaactg
     5461 gtacagactt tggttcaaag gtacgatcaa aggttgattc agaagtcagc tcatcagagg
     5521 ccagttcacc agtggccaga tcatcagagg ccagttcact agaggataga tcaccagagg
     5581 ccagatcatc agaggctgga tcagtggaag ctggtgctcc agaagccaga tcaacagaag
     5641 ttatttcact agaggctata tcatcagcgg cacaatcacc agaaatgggt tcaacaggga
     5701 ccggttcacc tgggatggga tcactagagg tgggatcacc agaggagaga tcatcagcag
     5761 tggcatcacc agaggtggca tcaccagggg acacatcacc agaaggggca tcaccagagg
     5821 ttgcatcacc agaggcagca tcactagagg agggctcgct agaggtggga tcaccagagg
     5881 tggaatcacc agaggccagt actccggagg ctggatctcc ggcatccaga tcaccagggg
     5941 ctggatcacc agaaactggt tcagcaggca gtttagagac tggttctgga gttatattat
     6001 ggggagacaa aggttcacaa tttaaatcat catcctgctt gttttttaca ttaacactta
     6061 ggatacaggg tgtttccttc cattctgctt tacttccttc aggatcaaaa caaatctctt
     6121 caatgccatt cacctcttcc ttgtctttaa tgtaatccat gttttccaaa ggtgaggttg
     6181 ggtccaaatc tccattttca tggttgccat ttaacagctt gacatcagtt ttcaacagtt
     6241 cttctttgac cttgtacact gcttcaagtt gctgacgatc actcactctc atcgtttttc
     6301 gagccttaaa gacttttttc tgaggttctt ctaaactgtc cattccccgg gaagcggccg
     6361 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     6421 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     6481 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     6541 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     6601 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     6661 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     6721 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     6781 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     6841 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     6901 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     6961 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     7021 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     7081 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     7141 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     7201 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     7261 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     7321 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     7381 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     7441 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     7501 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     7561 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     7621 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     7681 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     7741 actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg
     7801 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7861 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7921 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7981 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8041 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8101 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8161 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8221 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8281 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8341 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8401 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8461 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8521 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8581 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8641 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8701 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8761 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8821 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8881 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8941 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9001 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9061 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9121 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9181 agtggcgata agtcgtgtct taccgg
//
