LOCUS       sequence-406582-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ATP5B_VWA3B from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ATP5B_VWA3B
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4400..5110,5111..5113,5114..5116))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4442..4463)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4791..4810
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5050..5071
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5113..5122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5198..5401)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5227..5247)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5402..5705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5962..6006)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6191..6424
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6784..6901
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7393..7518
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7565..7822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7824..8203
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8234..8338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8339..8407,8408..9199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8557..8576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9370..9639,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaca
     1801 gagtttcggg cacagagcgt aatcctttgg acgagtgtgt cgcttgtaat gtgcagggta
     1861 aggcagcgcg aggtgggggc gttgcaataa tggcgtgact ggataatccc agacgcccgg
     1921 cggcggggaa atgcaggtgt gcagtgcggg gttccggatg ggtcccttga cagctcccgt
     1981 gactactgat tccatgcgaa ctaccgtcgc tatcggacgg ggctgcctgt tgaagaggct
     2041 gcttagcagg acgtttttta cggcgaggtt tctcgcgacg cggctcgcgg ctatcctgcg
     2101 tatccgcttc cttcaacggc caaaataaaa acgctgagtt acgagcttcg acgtcttcca
     2161 cctctgggtc ttctgggatg ggtggagatt taatgtgtga acaggataac gcgtatttgt
     2221 tctgactgat tttaatcagc gcactacgag ggcagaattc acggcgatta ttacacttaa
     2281 gcactgtata aaatttttcc gttgccacat gcttattcgg caatgcgatg acaatagccg
     2341 gtacataaaa gtcaaatcct ttgggaatca cgatcttagc gaagacatag tcaccaacct
     2401 gcagcagggg gcacggcatg gctccaccga ccggagtgat aaaacttgtg gagacaacct
     2461 tcgtgtctcc atagctgaag ccgactaacg cttgtgtacg tgaaacgcat tttttaacga
     2521 caccgggaaa ataaaaacca ttctcatcac agcgggcaat aactttttga ccctttaatg
     2581 ggtctggacg cttagatttt gttttttttg tggggttccc ctgaagtttc tgctgctccc
     2641 caggagcttt attagcatag ttcttctttg ccgcctcctg taactccatt gcttgctgaa
     2701 tatacatctt acctgccagg atttcggatt ccagcatgga aagctcctta agcgagatgg
     2761 gccaatttaa gcgttccaac gcttgattaa gcaccgtctt attctccagg tattgaatag
     2821 gtttatttgc atccaatttc tccttttcct cctgagaaag agcgcgccaa acgattttat
     2881 tcaagcgttt ctcataagat tgaattgctt gttctacttt gcgcttgtag atattaagtt
     2941 tggccccttg gggattaatc agagtcattt tgttcgtgtc gttacacacg tgtgccaatg
     3001 ggaatacttc gtcgaagact ttgctaacaa catgtttatc cagtacaggc acataggtgg
     3061 acgagtgagg cacagccgca accgataacg catccatcag tgttaatttt ttcgccacaa
     3121 ggccatacgt cttcagccag ttttccgatg aaacatccga agaatcatga tcgtacactt
     3181 gtgaaccttc acgggtcact ttttcgctgc tcttgtcatc caaccactcc tctgccaaaa
     3241 ggatagacat ctctttatcg ctcaaggcag tgcgacgact cgatgcattg ctcaggccgt
     3301 ccttgcgctc agagcaggct gatgagcgca aactactcat ttgagaacgt aacaaagagg
     3361 ttttcgtact ctcagcatga aggacctttt ttttctgcac tttttggtct gaaagtcccc
     3421 atggaccctt aagcatgtta agtgaagagg tttgcgttga atcaacatca gactgtggct
     3481 tcgcacactt ttcaggggtg gagatcatac tacaaatctc tttttgatgc tttgaatctc
     3541 cgtccttctc tgcgttgtac caccagtcca taatcaggct ttcgctatac aagtcctgca
     3601 ttttctctaa atcagaatgg ccttgttcca tttccttaac aagtaaggtc agatcttcat
     3661 tttgtacggc ctcgggtgga gtggggtcct tacacccaaa attataaaaa tggaactcac
     3721 cacccgtcag cgccgccacc tcctttaaaa aacggtttgc gatttcatca ttgtaattga
     3781 aagaaatggt ataaatcggg atttcctgga agcgtttgac ttgatcaata acggtttcgg
     3841 gcggctgatc agggcgccca tcggtaagaa ggtaaattgc ctgggtctct ttatcggcaa
     3901 aagcggtttt caaggccgac agagtattgg tcgaactgcc gatgcctacc gggatcttga
     3961 ttggagcacc actatccaaa accttctggc cgcggacaag gccctccgta ccgtccatag
     4021 caatagtgcg aaccgtggat tcgcccaggt gttgtgccac ttccaaaact aagcgcgttt
     4081 cgcgaccctg tacttcaagt gcgttcaaaa ttggcggcag cccctcatca aattgtacgt
     4141 ctacaaccgc cccgatgacg gcaacaatgc gacctgtagc agcccccgcc ttagggctag
     4201 gacttgtctg cgcggcgtaa tcacgaacgg ggtgtacggc ggtcggagcg gcgcgcagta
     4261 acagctgagc cggcggaagg ctcgctgacg gtgtaagacg gcgcaaagca cctgaagccg
     4321 gagctgcggc cacacgtcct acgaagccca gcattccccg ggaagcggcc gcacttcccc
     4381 cggagcctcc gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     4441 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     4501 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     4561 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     4621 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     4681 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     4741 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     4801 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     4861 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     4921 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     4981 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     5041 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     5101 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
     5161 gaattcggtt cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc
     5221 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     5281 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     5341 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     5401 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5461 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5521 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5581 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5641 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5701 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5761 aactagcacg cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa
     5821 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     5881 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     5941 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     6001 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     6061 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     6121 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     6181 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     6241 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     6301 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     6361 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     6421 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     6481 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     6541 cctcatatct cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg
     6601 tctacttgcc acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc
     6661 ttttcttctt ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc
     6721 tttccagagg agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac
     6781 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     6841 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     6901 attgtggatg aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac
     6961 ctgtcctata attttcttta attctttatt catagattct actactcctt gactttgggg
     7021 attgtaggga atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt
     7081 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     7141 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     7201 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     7261 gccagtatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     7321 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     7381 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     7441 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     7501 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     7561 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     7621 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     7681 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     7741 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     7801 agtcccgccc aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc
     7861 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     7921 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     7981 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     8041 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     8101 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     8161 cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt
     8221 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8281 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8341 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8401 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8461 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8521 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8581 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8641 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8701 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8761 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8821 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8881 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8941 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9001 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9061 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9121 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9181 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9241 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9301 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9361 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9421 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9481 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9541 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9601 agtggctgct gccagtggcg ataagtcgtg tcttaccgg
//
