LOCUS       sequence-406583-        9453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, BCL9L_CBL from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_BCL9L_CBL
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(115..219)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        250..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             631..888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    935..1060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1552..1669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2029..2262
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2447..2491
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2748..3051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3052..3255
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3206..3226
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3331..3340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3337..3339,3340..3342,3343..4053)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3382..3403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3643..3662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3990..4011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6720..6739)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6785..6840
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6839..6858)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7201..7217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7201..7217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7214..7236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7225..7241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7249..7255,7256..7273,7274..7279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7294..7315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7374..7569
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7420..7555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7575..7709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7612..7631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7666..7685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7777..7794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7948..8536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8028..8047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8707..9453,1..45,46..114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9330..9349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
       61 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      241 acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg gaggcgtggc ctgggcggga ctggggagtg
      661 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
      721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      901 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      961 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1021 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1081 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1141 acatatagta tgggcaagca gggagctaga acgattcgca gttaatactg gcctgttaga
     1201 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1261 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1321 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1381 gaaaaaagca cagcaagcag caggatcttc agacctggaa attccctaca atccccaaag
     1441 tcaaggagta gtagaatcta tgaataaaga attaaagaaa attataggac aggtaagaga
     1501 tcaggctgaa catcttaaga cagcagtaca aatggcagta ttcatccaca attttaaaag
     1561 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     1621 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     1681 cagggacagc agaaatccac tttggaaagg accagcaaag ctcctctgga aaggtgaagg
     1741 ggcagtagta atacaagata atagtgacat aaaagtagtg ccaagaagaa aagcaaagat
     1801 cattagggat tatggaaaac agatggcagg tgatgattgt gtggcaagta gacaggatga
     1861 ggattagaac atggaaaagt ttagtaaaac accataagga ggagatatga gggacaattg
     1921 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1981 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     2041 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     2101 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     2161 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     2221 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     2281 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     2341 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     2401 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     2461 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     2521 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     2581 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2641 ccacctccca accccgaggg gaccgagctc aagcttcgaa cgcgtgctag ttattaatag
     2701 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2761 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     2821 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2881 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     2941 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3001 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3061 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3121 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     3181 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     3241 tatataagca gagctcgttt agtgaaccgg gctcgtttag tgaaccgaat tctgcagtcg
     3301 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3361 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3421 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3541 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3601 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3661 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3721 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3781 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3841 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3901 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     3961 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4021 gggatcactc tcggcatgga cgagctgtac aagggcggaa gcggaggctc cgggggaagt
     4081 gcggccgctt cccggatgcg catccttgcc aacaagaccc gcttaccaca tccacgtcgt
     4141 cgcgaagccc ctggcagtcc accattgtcc ccgcgcgggc attgccctcc cgcccccgct
     4201 aaaccaatgc acccggaaaa taagttgact aatcatggta agaccggcaa tggcggtgct
     4261 cagtcacagc accagaatgt gaatcaaggc cccacatgta atgtcggttc caaaggcgta
     4321 ggagctggca accatggcgc gaaagccaac caaatcagcc cctctaattc gtcgttgaaa
     4381 aaccctcagg caggagtccc accattcagt agcttaaaag gtaaagttaa acgcgaccgc
     4441 agtgtatcag tcgattcagg ggagcaacgt gaagcaggaa caccctcatt ggattcagag
     4501 gctaaggaag tcgcgcctcg ttctaagcgc cgttgtgttt tagagcgcaa gcagccgtat
     4561 tccggcgacg aatggtgcag tggcccagac tctgaggagg atgataagcc aatcggagcg
     4621 acacataact gcaacgtcgc cgatccggca atggcggccc cgcaacttgg gccgggacaa
     4681 actacgcagc ttccactttc cgaatcgtcc gtccctgggg cgcctcacgg gcctccgcct
     4741 ggcttacgcc ccgatgcacc aggaggaggt ggcggtgggg ggggtgttcc cggtaagcct
     4801 cccagccaat ttgtgtatgt gtttacaaca cacttagcga acacggccgc ggaagcagtg
     4861 ctgcagggac gtgcagattc aatccttgct taccaccaac aaaatgtgcc acgtgcaaaa
     4921 ttagatcaag caccaaaggt gccacctaca ccggaaccct tgccgttgag tacaccttct
     4981 gctggcacgc cgcagtcgca acctccacct ttgcctcccc ctccaccccc tgctcctggg
     5041 agcgctccac ccgcattacc ccccgagggc cctccagagg actcaagcca ggacttggcc
     5101 cccaattccg taggcgctgc atcaacaggc ggcgggactg gaggaacaca ccctaacact
     5161 cctacagcaa ctaccgcgaa tgtagagcgt ccaccaagcc cgttttcaat ggcaccacaa
     5221 gcatcactgc cacccgtccc gccgcgctta gatcttttac cgcaacgcgt gtgcgtaccg
     5281 tcgtctgcat cagcattagg aacggcctcg aaagcggcat ctgggagcct tcataaggat
     5341 aaaccattgc cagtgccccc taccctgcgt gatttgccac cacctccgcc cccagaccgt
     5401 ccctacagtg taggtgccga aagtcgcccc cagcgccgcc ctctgccatg cacccctgga
     5461 gactgtccgt cacgcgataa actgcctccc gtgccgagca gccgcttagg cgacagctgg
     5521 cttccccgtc ctatcccgaa agtgcctgta agcgctcctt cgtcttcaga tccgtggacc
     5581 gggcgtgaat taactaaccg tcatagcctt cctttttcct tgcccagcca aatggagccg
     5641 cgccctgacg tcccccgctt ggggtcaacc ttcagccttg atacatccat gagtatgaat
     5701 agctccccgt tggttggacc ggagtgcgac cacccaaaaa tcaagccatc cagtagtgcc
     5761 aacgcgatct actccttagc cgcacgtccc ttaccggtgc ctaaactgcc gcctggcgaa
     5821 cagtgtgaag gggaagaaga caccgaatat atgaccccat cgagccgtcc cctgcgtccg
     5881 ttagatacga gtcagtcatc acgtgcatgt gattgtgacc agcagatcga ctcgtgcact
     5941 tatgaagcaa tgtacaacat tcaatcgcag gcgcccagta tcactgagag ttctactttc
     6001 ggggagggta acctggcagc tgcacatgcc aacactgggc cggaggaatc tgagaatgag
     6061 gatgatggat acgacgttcc gaagccaccg gtgccggcag tcttggcccg tcgcaccttg
     6121 agcgacatct ctaacgcatc gtcatccttc ggttggctgt ctttggatgg tgaccccact
     6181 accaatgtaa cagaagggag ccaggtccct gaacgcccac cgaagccatt cccccgccgc
     6241 attaattccg aacgtaaagc tgggtcctgc cagcaaggta gtggacctgc tgcttcagcg
     6301 gccacagctt caccgcaact tagcagcgaa atcgaaaatt tgatgtcaca aggatattcc
     6361 tatcaggata ttcagaaggc tctggttatt gcacaaaata acatcgaaat ggccaaaaac
     6421 atccttcgtg agtttgtctc gatcagcagc cccgcccacg ttgcgacctg aaccggttct
     6481 agataagatt acggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6541 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatct
     6601 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6661 ctaactaggg aacccactgc ttaagcctca ataaagcttc agctgctcga gctagcagat
     6721 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     6781 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     6841 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     6901 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     6961 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     7021 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     7081 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     7141 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     7201 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7261 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7321 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     7381 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7441 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7501 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7561 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7681 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     7741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8641 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8701 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8761 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8821 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8881 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8941 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9001 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9061 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9421 gagatccagt tcgatgtaac ccactcgtgc acc
//
