LOCUS       sequence-406588-        9894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, BCR_JAK2 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_BCR_JAK2
FEATURES             Location/Qualifiers
     source          1..9894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             3808..3822
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     CDS             complement(join(4655..5365,5366..5368,5369..5371))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4697..4718)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5046..5065
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5305..5326
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5368..5377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5453..5656)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5482..5502)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5657..5960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6217..6261)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6446..6679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7039..7156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7648..7773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7820..8077
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8079..8458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8489..8593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8594..8662,8663..9454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8812..8831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9625..9894,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaac
     1801 cagccatgtt gtcacggatc tgatccacgc gtaaggccaa atcgcggaat gagggacgtt
     1861 ggttgacgtt gttgttccaa cactcggtca tgatcatgta gatctcatct gggcacccat
     1921 cagggcgggg caaacgtccg ttgtttttaa gtaattcaat aagatggaat acgatcatct
     1981 gaccttgttt gtcattgccg atcatacgca tgaactccgc cggtggcgat ttggattttt
     2041 cgatgtaagt gaacaactcg tacaagacga cgccaaagga ccagacatct gacgcgacgg
     2101 agaacttcga ttccgtaaga gactctggtg cataccagaa gataggggac tctcccggtt
     2161 ccttgacctt atagtattcc ttgtcttgag gcagaacctt ggtcaacccg aagtcgccga
     2221 ttttaacgcg gttctcgttt tcaaccaaga tgttgcgcgt ggccagatca cggtggatgt
     2281 agcgcttcgt cccaaggtat tccatgccct tgcaaatctg tgatgtatac tgtaacagct
     2341 tgatgtggtc aatgcgctcc ttatgttttt gaagataatc gcgaaggctt ccatatggta
     2401 agtactccat aataagtttt aagttacgac ggcctgcgct gtaacacacc cccttatact
     2461 tcacaatgtt atcatgctgc agtgacttca agatttcaat ttcacgctca aaatcgcgca
     2521 agtgctcttc agtgctgtgc tgcagctttt tgaccgcgac tacctctcca gtgttgtctt
     2581 gtaagggatc atagcggcac atctctacac ttccgaaatt gccttttcca agttgttgaa
     2641 gaaactttaa gtgacgctct tcgaattgag tagggtcgcg gtcctcaaat gcacctgaga
     2701 atcccaaagc gccaatacgc atgttaggca gcatgtcgtt ctctgttaac agctcatagt
     2761 cgggggtaaa aagagagttc aggtcgcgga tgatcgcgcg gaatgagggg cgaaaatcag
     2821 gctcatagtc catgcagtta ttaataaggt tagccagttc agcccattta ggagctggca
     2881 attggtggcg atcctcgtag aattgcagct tacgctggga gtccaatgct gacaatggtt
     2941 tgtcgcctcc actacagatc tcccaaagag tagttccgaa cgaccatttg tccgtggcaa
     3001 ggtttaagtt ctttgggttc tcaatgcact ctggcggaac ccagggaata cgttcctgta
     3061 agatatcttt aggcagaacg gtgatggaga tgcccggatc gcttaactta ataaatggag
     3121 gattacccgt tttacggtcc tcctcacgga taagaagaat gtttttagca cagacattac
     3181 catggattaa agtattctcc tctaagaagt gcattgccca agctaattgt ttcgctactt
     3241 ccaatttcca cagaatattg atacagtttt tgtttttctt caaatacgtg tccaatgacc
     3301 cgaactttac aaattcctgg actaagatat ccgcatctcc gtggaatgcc ccttcgccat
     3361 catttggcca gatttggcct gtcttgcgca cgcccacaat agtagcctcg cttacaacaa
     3421 cgggacaatg acggtgacgt ttgtggcatt gaggtgttgg tggactagat gagtcgaaac
     3481 tttgctggct attttgagac ggggaacggc ttttatcgcg gaacatgcga tacgtcgtag
     3541 gagatgggga aacacggcta gactgtccac tagaaaaatc ctcttcacta gacgtaaggt
     3601 tttcgtttga gctacaatcg ggcgtatagc caccgccgca gtcttcgaag ctacgagggg
     3661 agtatgagcg gcgcggccac gtcaggcgct tttcttgctc agaggtgctc tgggagcgca
     3721 gaagtggccc cttcccctca ccttccatca taccgcccac gtagatactc tggtacggtt
     3781 gatactccag cggtggccag ggtgggcggg agccaccatt ggcgtcgatc aggttatctt
     3841 tcaggaagcg cggattaagc tctgcatctt cgtagtcacc atcgacaccg cagctagact
     3901 cggatgagcg cccgcgatag ggggggcggc tcgcatcacc aacggagcta ccggcgccat
     3961 gctgggactt cttacgttcc atttgcattg cttggctacc caaactgctg atacgatcgg
     4021 aaacctcttt atcattgacc ttaacaagcc cgcgttcatg atgaaattca acgttaacgt
     4081 aaaacggctt ttccgcgtca gcaccgggtt gcccatgtcc tttacggatg cgttcgaaat
     4141 tgctgcgcag agcagctacg ctcgcgggag gcccacggtc atcgcgctca ccagaggctg
     4201 ctgcgccagg gcgacgcgcg gtgccagggc gagctttgcc aggagaacct tccccatctg
     4261 gacgggcttc cggctcctct gctggagggg gatcggctcc atcggcgggg gccggttgcg
     4321 gacgacttgc tgaggcacgt ggctcactgg ctccatcggg cgcttgagcc gcacggcgaa
     4381 agccccaacg ttgacgatca taagactttt tctccttcgc aagaagcgtc tgtaagtaaa
     4441 tcatacgaaa gcgctcttgg tttacctcct gttccagacg acggattgac gccttacagc
     4501 gttccaattc ttgttcaata tcacccactg aacgtaattc catgcgcggc ggctcactgt
     4561 ctggaaactg agctttccac gcttccgcga acccaacggg gtcaaccatt ccccgggaag
     4621 cggccgcact tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag
     4681 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     4741 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     4801 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     4861 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     4921 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     4981 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     5041 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     5101 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     5161 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     5221 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     5281 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     5341 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg
     5401 gtaccgtcga ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc
     5461 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5521 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5581 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5641 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5701 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5761 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5821 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5881 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     5941 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     6001 ttgattacta ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg
     6061 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     6121 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     6181 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     6241 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     6301 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     6361 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     6421 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     6481 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     6541 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     6601 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     6661 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     6721 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     6781 acttctccaa ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt
     6841 ctaatcctca tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc
     6901 cctaatgatc tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac
     6961 tactgcccct tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct
     7021 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     7081 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     7141 cccttttctt ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc
     7201 agcctgatct cttacctgtc ctataatttt ctttaattct ttattcatag attctactac
     7261 tccttgactt tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc
     7321 ttttttctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     7381 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     7441 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     7501 tgatgtttct aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac
     7561 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     7621 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     7681 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     7741 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     7801 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     7861 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     7921 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     7981 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     8041 ggctcgccac tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat
     8101 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     8161 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     8221 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     8281 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     8341 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     8401 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
     8461 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8761 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9001 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9301 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9421 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9481 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9541 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgg
//
