LOCUS       sequence-406594-       11988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, BRD9_NUTM1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_BRD9_NUTM1
FEATURES             Location/Qualifiers
     source          1..11988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5397..5408)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(6749..7459,7460..7462,7463..7465))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6791..6812)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7140..7159
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7399..7420
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7462..7471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7547..7750)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7576..7596)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7751..8054
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(8311..8355)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8540..8773
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9133..9250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9742..9867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9914..10171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        10173..10552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10583..10687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10688..10756,10757..11548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10906..10925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11719..11988,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggtctact
     1801 ggctacgacg tcgtttcttc cttctgcccg tgacaaaact gtcacaccgc cttttacgag
     1861 gctgtgaggg tcgaactact cccagagcta ggggtttctc cctggggacc ccaagttgag
     1921 ctcctgagtg gggtgtcttt tcagtagggg ctggacctcc agctagagct cgcttcccag
     1981 acttggcaac aggatatgga agtttgctgg ggccttttgc tccagcaggg agcaggtggg
     2041 atgctctctg gctgccctga cctcctgagg catggtgagg actgagggga tgctcccttg
     2101 gtgagaggct aagtttagag gccaagaggt aagcaaagtt ggagagttcc tcatcctctt
     2161 cctctgcttc ctttttcttt ttctcctttc ctttggccct gtctgctgac ctgtgtgttt
     2221 tactaacaga agtttctctc ggaactatgg cattcctggt tccccttcta ggcaaagtgc
     2281 ttccaaggcc ctggtgagaa gatgttgctt tgggagtccc agttgtgtaa gactcctgac
     2341 tctcttgtaa aggagcaagg tttttaggct tggacagatc aggatccacc ctgccttctc
     2401 cttgggggtc ataagaatga acattcagtg ggcaggtgtc ctcgctgacc cttagttgga
     2461 ggccacagtc atctttaaca tctagtatgt tgacaggttc tatggtttcc aacagaggag
     2521 aaaaggaatt gcctctgctc cctgcctctt gactggcttc aggcaaggag gaggcatcct
     2581 cagtccccat gataaggaga ttgccttggc atgtgggtgg aagggtttct ttggaagacc
     2641 ccagtgtgga atcaccactt tctagcaatg ggaagcaacc ttctgcccac agatcactgg
     2701 gatgccctac ggtttcacag ctctgttctt gattttcttt ggacctcaag ggtgggctgt
     2761 tggccctcaa aggtcctggc aagctgcaat agtttctctt ttctagggca gctgccgcaa
     2821 ctgtgcctcc acagggaatc acactgctct ccgtatcccc aggtactagg gtttctccag
     2881 gacccaggga aatgttgccc ctggagccca gtccctggca gccttcctga tactcagtta
     2941 cacacttctc aacttggaag ctctctatga cctcctctat ttgtacaggc agctccaagc
     3001 ctacagcatc catctcactg ctcagccaaa ctcctgggct gagacagaca tcgtccctct
     3061 ccccagctgc tctaggggaa ggattctgat cataactctg agccatgggg gtacctctgt
     3121 catctcccca catggctcct gaagagcctt cccagggcac tgctaaaggc tccttccctt
     3181 gaggaagcac tccacgaccc cctgtttgtt gtcctttctg caatcccagg acttgctttt
     3241 ctaacccttg tccttgcaga ggagccagct ctgcaagtcc agcatccaaa ctgggagtgc
     3301 tctcaggctg gaagcctccc tgccaacaaa ggggcagagc ctctgaagtc ctatcagcca
     3361 ccaggcagtg cccaggaggc ccagcgcctc ctagatggcc atcctgatgt agagatacct
     3421 ggtttataac cttcccagac cctctcctct ccacacccaa gggtcccgga gttccctgtg
     3481 gagctgcaag ttctggctgc aggtcccagc tgctggggga tggcagagtg ttcccatccc
     3541 tgtgcatccc tctggggcta tctagggctt gctcctgccc accatgcact tctcttgccc
     3601 gttttcctga agaagaaacc tttccaaggc aagcggcgcc cccagccccc tgaagcccag
     3661 gtgagggccg aagccgccca tccccatctt catcctcaac agaaccagaa ggacttgagt
     3721 ccaactgagc gccactgaaa cttggaggcg cctctgcatc ttcctcctct tccaaggcca
     3781 tgagtcgctt ctggaccagc tgggcaagag tgagtccttc ttcttgctct agctcctcaa
     3841 ttaaggccaa gggatctctc tgtttttctg gggacagcag atctgccaga aattgagggt
     3901 gaatgacagc ctccaccttg gagacaaaga ccttctgaga acacagctca ttgatgtagc
     3961 tcaggagacc tggatctgga tacatccctt cctcctcctg ctgctgccct tcttcctctt
     4021 gttttccatc tgactcccca gtggccaagt gagtccccac cagccattcc atgatgtcaa
     4081 catactcctt cacagcttct ggtgggatct ccttgggtgc ctcaggagca ggaggtctct
     4141 gggctttacg ctgacgccgg cggggggccc gtgtcttgga ggctgccttc ttcggaatgt
     4201 acactggctg ctggcaaacc tcagaggcca ggggccctag aggatcaagt ttcaaagggg
     4261 ttgcaggaga caggccctga gacccattca tcagctgtgt gttctgaatc tgcatctcct
     4321 cagcctcaaa ctccatgaac ctttctgcca tctcataaaa gatcatccgg tcaaagttgc
     4381 tggtgtgctc ccactcctgc acagccaatg gcagtccctc ctccagggtc atagtgggct
     4441 tcagccgggc cagggaacga agcactggga taagaaaaca ggaaagagct tctgtgtcag
     4501 gactctggga taggtgcctc cgggccaagg ctttgtaacg ctgccactga cggaagttct
     4561 cataaacgtc cttggaaatt ttggagcggt cacctaggga aggcttggat agagtggcca
     4621 caggacctcc ttccccggtt gtcccatgtg gccctggcca agctttttcc aggggcacaa
     4681 tggggaccag ttgagcaact ggtggtggag gctgaggcgg aaggcctgga ggaccctcct
     4741 ggctgacacc aacagcctta gagggcagaa tggtcttcac attagatgct gtcacaaatg
     4801 gaggtgcagg accctcaagg cctccacagg gagtgcccgg ggcagtcgaa ttgagggcag
     4861 tctgagtaag gataaagttc tgagtttgag agggctcagc tgacccccct tctgtcttga
     4921 ctttgacaat gaccttgcca gccccagccc cactgaggca agggcccccg tcccctgtca
     4981 ccaacagtga gctggggaaa gcagagagca tcagagggtt gtctggagag aatactgaag
     5041 gcatgatggg ctgtggaggt ggctccctgg gtgggtggtc tggtgggtca gaagttggtg
     5101 ggagaaaggg aagtgcaggg gatggagaca gggcggcact aggtttcatg ctcatatccg
     5161 gtcccggcaa tgcagatgcc agagagctga gcatggagat atccacagaa acgtcgggat
     5221 aggacttcat cgacatgaac tccagaacag agctgctgct gtctcctagg gtgtccccaa
     5281 ccttggcttc atctggaggc ttcatgggaa catttcttct ctgcttcagc tggaagagcg
     5341 tcctagagtg gtctccgcct gtgatctggt ccaggaggtc gtccaccact ttcttgctgt
     5401 agctcccagc atccttcaca aactcctgca ggctcagcgc acactgcacg cctgtctcat
     5461 ctccgtaggc tgagtagagc agctccatct cgtccgactt caagtcgcca aatactgaat
     5521 tattctgcat cgaaagcgca gtagtggcac tggagagaaa ggtgactttg tttcttctct
     5581 cgtctttgaa gcccagcgtg gtgaagcctg ggagtagctt actggagagc gagctcaagt
     5641 ccaccgggtg ggtctcctcc tcatcagcgt ccggctcggc cgtgttgacc acgctgtaga
     5701 gcaggctccc gtccccgttc ctcttcagat agcccatctt gccgcctggg aggaaccggt
     5761 tgatcctgtc ccgagcttcg tcagctgcgt gctccaccag cgccagcacg tgctcctctg
     5821 cggtactgtc cgtcaagctg caggcattcc cttcaggctc aaacatgcag ctgataactt
     5881 ctctactcgg ctttttggat ttcttggcag tttctacttg tactggtaca acttcaggga
     5941 caggttcctc aacagctgta tcttcattgc ccaaaagagc tgcctgtttg ctcatcatct
     6001 taaagcctgc gtgaaggatc ttcttcgcca acttgtagta cacggtatct ggcctattgt
     6061 atgtcattgc attatcacac atcagcttga aatctgcctt aaattccgta actgacttgt
     6121 attcattagc tacaattttg tctttcatgg tgccaaaatc catgggatgt tttattatca
     6181 ttgaatatcc aggagcaatt gcatccgtga caggaaaagc aaaaaatcca tggggatctt
     6241 ttctctgaag ctggcggagg aagtgttcca ggagttgctg aataggtgtg ctctcatttt
     6301 cggctggctg tgtccggcac gctcggactg gccgatctgg gggcggctcc acctccacct
     6361 tcttcccagg atcaaagtcg tcagcctctc cctccgtgtc acagtgctcc ctctctcgct
     6421 tccgcttctt ctcttccttt cgcttccttc tttcctcatc gtccagatgc ttctccttct
     6481 cggacttctt cttcttcttc tttttctttt ctttgtgcct ctctcgctca tggtctgacc
     6541 tgtcatcata gtaactggag tcgtggccgg atcctgagag ttcagtcact tcacttcctc
     6601 cgaccttcag gactagcttt agaggcttct ccaggggctt gtcggcataa tcctcgtagg
     6661 acgagcgcca ctcggccttg tgcttcttgt gcttcttgcc cattccccgg gaagcggccg
     6721 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     6781 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     6841 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     6901 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     6961 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     7021 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     7081 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     7141 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     7201 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     7261 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     7321 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     7381 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     7441 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     7501 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     7561 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     7621 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     7681 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     7741 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     7801 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     7861 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     7921 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     7981 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     8041 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     8101 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     8161 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     8221 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     8281 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     8341 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     8401 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     8461 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     8521 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     8581 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     8641 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     8701 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     8761 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     8821 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     8881 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     8941 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     9001 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     9061 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     9121 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     9181 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     9241 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     9301 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     9361 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     9421 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     9481 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     9541 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     9601 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     9661 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     9721 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     9781 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     9841 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     9901 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     9961 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
    10021 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
    10081 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
    10141 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
    10201 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
    10261 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
    10321 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
    10381 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
    10441 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
    10501 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
    10561 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    10621 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    10681 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    10741 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    10801 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    10861 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    10921 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    10981 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    11041 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    11101 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    11161 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    11221 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    11281 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    11341 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    11401 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    11461 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    11521 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    11581 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    11641 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    11701 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    11761 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    11821 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11881 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11941 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
