LOCUS       sequence-406598-        9939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, C11orf95_RELA from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_C11orf95_RELA
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1799..2170)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             complement(join(4700..5410,5411..5413,5414..5416))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4742..4763)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5350..5371
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5413..5422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5498..5701)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5527..5547)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5702..6005
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6262..6306)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6491..6724
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7084..7201
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7693..7818
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7865..8122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8124..8503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8534..8638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8639..8707,8708..9499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8857..8876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9670..9939,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 tgctgatctg gctaagcaaa gcggagaagt ccatatcagc aatactacta aagtcttcgt
     1861 cgccgcttaa taagccgttg ggcaagcccg gtgcacccag cggcgctgga gccgggtccg
     1921 gcggacgctg cgcgcccgtc accagacggg tgatcgcttc cgggtattcc atcagcatcg
     1981 gttcggtggt gtgcggggcc accggaatgc cctgatttag cagctgttgg aactcactgt
     2041 tgtccacgct tgccaagtcg gtaaacaccg ccgggtccgt tgagttgccc aacagcgcgc
     2101 caagatcctc atcatcaaat tgcagctgca gcagcgcctc cgacagcgta ccctcacccg
     2161 cctgtgtcgg cttcggggcc ggaggcgcga ccgcttgagg cggacccggc gccaggactg
     2221 gaaccggtgc cggcgcttga gccagagcgc ttaccatggc cggagccggc gccggagccg
     2281 gggcttgggg cagaacctgc ggtggcgctg gcgccaaagc gctcgcttgt gagatttgac
     2341 cagacgggaa caccatcgtc gggaactcat catagttaat ggtggacaaa gatgaagtaa
     2401 acgggtaggg ctgaggcgcc ggcttcggca cggaagcaga gctccgagac ggcaccgcaa
     2461 tgcgacgcgg gggcggacgt gggtcggtcg ggccgctaaa cgggctcttt ttcatgatgc
     2521 ttttaaaggt ttcgtacgta cgtttgcgct tctcctcaat gcggtggcgg tcgtcggtgt
     2581 ccggcagata ttggaactcc atcggttcgg acagttcacg gtcgcttggg cgacgcagct
     2641 gcatgctcac gcgcaccggc gcttgcaggc tcggatctgc gtacggcggg gtgcggaaca
     2701 cgatcgcaac ctgacgatga acatccgctt ggctaaagga gccacgtgct tcccaacctg
     2761 gaccggtgaa ataaacctca atatcttctt tctgaacttt gtcgcacaac agaaagatct
     2821 cgtcaccacc cagacagcta ccactgttac gattaacacg gcagatcttc agttctgcgg
     2881 tgttgggagc acggttatcg aagatcggat gggaaaggac cggcggcaga cgcagagggc
     2941 gaccgctagg atcacgaacg gtgacttgaa agcataaacg cacagcgttg aggtcgtagt
     3001 ctccacgctg ttcctctatc ggaacctgga acgggttgtt attggtttgg atgcgctgac
     3061 taatcgcctg ctccaaatct ctttttttaa cacactgaat gcccaggttt tggaagctat
     3121 gaatgcagcg gtccgggcag agttccgctt cataaaaacc atcacgacag tccttaccta
     3181 ccagttcatg cgggtgtggg cggtgaggcg gatctttggt caccagacta atacgcaccg
     3241 tacccggacc ggtataacca ttaatcttga tggtcggatg ggttttcgtg gtgtcagtgc
     3301 tgcgctctcc cgggatggaa cccgcggatc tgccctcgca cttgtaacgg aaacgcatac
     3361 cgcgttgctt cggctgttcg atgatttcaa catatgggcc tgacgcctgc gcgggctcag
     3421 ccgggaaaat taacggaaac aattcggaca atggcggaag atccgggtcc gcgcaaaccc
     3481 aatgaccgtt ctcgcacggt ggaatctgct caccacgcag cggggacgcc aaggaaccgc
     3541 cgagcggaac gctgtccggg cccgcaccac cagcagtaga agcgctatct ctgcttttac
     3601 cggtcagatc ttgcggagcc aggtcgtcgc ctggcgggga ctgagtcagc tcggacagcg
     3661 cctccggctg accaccccac gcctgcagca aagcgctacg ttgcggaccg gataaaccca
     3721 gagaacctgg gtgcacttcg agcacgtggg cacggatgtc atccaggtga agggacggca
     3781 gcgccctacc acatgccatg cacaccaggc gattaccacg cggatcgtaa tccattagac
     3841 attccgcgcg gaaccagttc tgcaggcttt ctttcaggcg acgttcaaga cgacgggcac
     3901 ccaaaccccg agaaccgcca gcacgtctgc tggcgctcag acgcaggcgg cgggcacgcg
     3961 gggcaaccgg accaccacgg cgttgacgac ggcacccgcc acccgctggt gctttgcccg
     4021 gacccttcgg cgggcaggcc gggacgccgg cgccttcctc ttcctcttct tcctcttcct
     4081 cctcttcctc ctccgcacct tgcacgccca gaccttccgc ctcaccgcac gcgcccagac
     4141 ccaggtgtgc atcccagcta ttgctgatca cctctttttc tctcgggctc caatgcaggc
     4201 tgtaagggtg tttttgacga atatggcgct tgatggtgga cagcttcagg gttgccagtg
     4261 aagagccgca gaccatgcaa accataccat gacgcgccgg attgaaatcc atcagatact
     4321 ccagacgcca gtgatcatgg tagtagcgac ggtggtcacg gcccgggata cgagacttac
     4381 ccgggcggct tgcgcgtgcc tcacagtgat ccgaatactt acggcccgag ctcgccggac
     4441 cacgcgcacg gctagacggc agtggcgcgc tgccccatga gccgagagcg ccaccctcca
     4501 gctggagatc ctggcccccc tcgtcctcct ccggttctgc agagccgcta gaacccgccg
     4561 gtggaagtct acggccgcgg gccgatgcaa ccgccggtcc cgggccacct cggcctccgc
     4621 tcgatcttga cctgtgatct cccccgggtt ccattccccg ggaagcggcc gcacttcccc
     4681 cggagcctcc gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     4741 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     4801 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     4861 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     4921 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     4981 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     5041 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     5101 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     5161 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     5221 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     5281 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     5341 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     5401 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
     5461 gaattcggtt cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc
     5521 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     5581 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     5641 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     5701 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5761 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5821 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5881 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5941 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     6001 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     6061 aactagcacg cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa
     6121 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     6181 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     6241 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     6301 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     6361 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     6421 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     6481 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     6541 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     6601 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     6661 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     6721 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     6781 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     6841 cctcatatct cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg
     6901 tctacttgcc acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc
     6961 ttttcttctt ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc
     7021 tttccagagg agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac
     7081 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     7141 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     7201 attgtggatg aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac
     7261 ctgtcctata attttcttta attctttatt catagattct actactcctt gactttgggg
     7321 attgtaggga atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt
     7381 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     7441 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     7501 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     7561 gccagtatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     7621 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     7681 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     7741 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     7801 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     7861 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     7921 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     7981 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     8041 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     8101 agtcccgccc aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc
     8161 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     8221 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     8281 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     8341 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     8401 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     8461 cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt
     8521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9901 agtggctgct gccagtggcg ataagtcgtg tcttaccgg
//
