LOCUS       sequence-406599-        9972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CBFA2T3_GLIS2
FEATURES             Location/Qualifiers
     source          1..9972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4733..5443,5444..5446,5447..5449))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4775..4796)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5124..5143
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5383..5404
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5446..5455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5531..5734)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5560..5580)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5735..6038
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6295..6339)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6524..6757
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7117..7234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7726..7851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7898..8155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8157..8536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8567..8671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8672..8740,8741..9532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8890..8909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9703..9972,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaat
     1801 ttacgacagc gggtttaagt aacacagatc ccggtggaat cacaacccat ccgggggcaa
     1861 gtgcctccgg ggaactcgcc gcagagttaa cgccggtcga taaatctaac accgtaccgg
     1921 gaagaagggg aaggccgtcg ggactgggac ggctgcatga tgactcggtg cgttccaggg
     1981 gtgttttgtg tccttccatg ccaagagcgc gggttggtac agaaccgcgc cctttctctc
     2041 cctcagcttt accccctgcg gctcctgcaa gcaacgacac aactggtaag cccaaccccc
     2101 cggctccgaa gggggaggga agaagcgggt tttttgctaa gttcagaggc aacaccggac
     2161 ccggcaatcc agggcccatt ccccctccac cgccggagcc acccccgtta ccacaggcca
     2221 gcgcactcag atctaaggga ccgggcgcta atggtaatgg caaaccaggc aagcctgggc
     2281 ccccgaataa cgcggcaggg ttggggataa tgatctgggc tccgctgacg taaggacccc
     2341 catctggtgc cggcaatgga ggtttcgggg gagggcgcag ttgcaacagt tcctgttgct
     2401 cgtgagaaac gaaatggccg tgagccttaa tatgtttgcg aagagaagag ggatcggtat
     2461 aacgtttatg gcagcccggc attttgcagt agtacggctt atcgacgtaa tgcgtgcgag
     2521 tgtgtttaaa acgatcactc gaattagaat aacgcttgtt gcacccttcg tatgggcata
     2581 cgtatggctt ttccccagtg tgactgcggt tgtggatttt taagttctcc aagcggctaa
     2641 aggatttaga gcatgtggga cagcggtgcg gtttttcgtt tgtatgtgta cggatgtgaa
     2701 taagcatctt atagcgagca ttgaaaccac gtccgtgacg ggcacagcct tcccaatgac
     2761 aacaatatcc ggcgtctttc tcaggtttaa cgtgataatc attgacgtga tccaccagat
     2821 cctgcaacaa ctcaaacagc tgattgcatt tggcccagcg acacactaat tgtttaggca
     2881 agggcagatc gggactcagg catttatcct taggtggagt cagaaagctg gaggcgggca
     2941 ggtgtaaggc cccacctgag cccagcggaa ggaagaattg gaagctactt gggacgccat
     3001 ctaaatagcg caacggttga aaatcctccg atgaatcttc ctgctgattg atcacagtaa
     3061 gggcatcttc tgatgcttgg cgcttggctt cggctaaagc gcgttccatt ttggcacgct
     3121 ccgtcgtgat cagttcatgt gctttacgtt cagcatcgga cacggccttc tgcaattccg
     3181 acattgcttg gcgtttgacc tcattcaccg cttcctcggc tttacgccaa atatcttcgg
     3241 gtacataacc tgttaatgta cgtggcagaa attcacgggg aacatccagc tgtggtccct
     3301 ccggacccgc cgacgacgaa cggggacggg ccgcggctgg cgcagggccc ttctttgtgt
     3361 cttcagcatc cgaataacga cgcgcccaat ggttcagctc ctcacgatcc gcttcttggc
     3421 aacggcgcaa gacagttaat gaacgacgag tcttttcgac catatccatg atgcagttaa
     3481 gcaaattgtt caggtgcttc cactcctcag cccattcacg ctcggttaat ttatgatcga
     3541 taacttcctc ctgacggctg ccagggacta ctaatgggcg atgacgctca cgcaactcgc
     3601 gggggtccgg atgacgatac gcgtcgcgga agttatgagc catcgcaatg tcctcaagac
     3661 gatagtgggg cggaggagta ggttgcggtg ggccgtttga aggcgagtaa cgttgagcag
     3721 gattcagtgt gcagggacgt ttagacagat gctcggggtg cagaggatca cggtcgctcc
     3781 cattctcctt cgtgcggtcg ggggtgcggc gtttaccgtt ctcattgacc tctaacagca
     3841 attcgctact gtcaataggg ctcgatgcag acgcatccag taaaagttgc tcgtgttgag
     3901 ctaagtattg agccggagtc tgcttggcca gacgtgcgca atgtaacaat tcgcgctgca
     3961 gaagcggaag gttcgctttc aggaacggaa tgacgaacgg gcgcaagggg aaattggtcg
     4021 cctcttgcaa tttggaatgg aattcttcaa tagtcagcgt gctgtttacc aatcccaaca
     4081 caagggtacg tacgcgctcc ccgatttctg gggagatatc cgaaccaaac tgctgaagtg
     4141 tagtaaggaa acgtttaagt ttggaaagtt ggcgtgcccc acaggcgggc ggaaggtgtt
     4201 gtgtcgaaag gctcgcagtg ctagatgtcg cgggcccgtt actgaagcca tttggagtgc
     4261 atggagcgcc gttgatagca gtaggggaat ggctgctacc gttcattaaa agacagacca
     4321 ttgaccactt cagacacccg tgaaaacgac catgcggaag cgtagcaggt ccgtcctcac
     4381 gatgcgtgtg tggggtgaag gatggcggac gcgtcgcccc ctgagaggcc gcgggcggtg
     4441 gtgggggcat agagggcggt gtgctacgag gctgcgtctt aacttccgca ggagagtcgg
     4501 gcatcgctga tgctttagct ttacgatcga ctggagcagg cccgccttta cgcggaccac
     4561 ggggggcgga gcaaccggcg ctagccagca gcccagactc taaaactgga tgcgtttggc
     4621 tcatagatcc acatgttgac ccgctggccg acgaggcggc gcgatcacga agacgcgaag
     4681 ccggcattcc ccgggaagcg gccgcacttc ccccggagcc tccgcttccg cccttgtaca
     4741 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     4801 cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag tggttgtcgg
     4861 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4921 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     4981 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     5041 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     5101 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     5161 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     5221 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     5281 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     5341 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     5401 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     5461 gtggatcccg ggcccgcggt accgtcgact gcagaattcg gttcactaaa cgagcccggt
     5521 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     5581 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     5641 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     5701 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     5761 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     5821 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     5881 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     5941 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     6001 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     6061 gaactaatga ccccgtaatt gattactatt aataactagc acgcgttcga agcttgagct
     6121 cggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     6181 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     6241 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     6301 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     6361 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     6421 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     6481 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     6541 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     6601 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     6661 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     6721 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     6781 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     6841 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctt atggtgtttt
     6901 actaaacttt tccatgttct aatcctcatc ctgtctactt gccacacaat catcacctgc
     6961 catctgtttt ccataatccc taatgatctt tgcttttctt cttggcacta cttttatgtc
     7021 actattatct tgtattacta ctgccccttc acctttccag aggagctttg ctggtccttt
     7081 ccaaagtgga tttctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     7141 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     7201 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtac
     7261 tgctgtctta agatgttcag cctgatctct tacctgtcct ataattttct ttaattcttt
     7321 attcatagat tctactactc cttgactttg gggattgtag ggaatttcca ggtctgaaga
     7381 tcctgctgct tgctgtgctt ttttcttact tttgttttgc tcttcctcta tcttgtctaa
     7441 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     7501 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     7561 tatttgtcta cagccttctg atgtttctaa caggccagta ttaactgcga atcgttctag
     7621 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     7681 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     7741 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     7801 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     7861 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     7921 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     7981 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     8041 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc
     8101 ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctcccatg
     8161 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     8221 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     8281 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     8341 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     8401 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     8461 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     8521 gtcaataatc aatgtcgacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9961 gtgtcttacc gg
//
